{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T18:10:04Z","timestamp":1770747004603,"version":"3.49.0"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"3","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1989,3,1]]},"abstract":"<jats:p>Specific receptors for a recently purified and cloned monocyte-derived neutrophil chemotactic factor (MDNCF) have been identified on the surface of normal human peripheral blood neutrophils using 125I-labeled recombinant human MDNCF (125I-MDNCF). Competitive binding of 125I-MDNCF to human neutrophils reached a maximal level at 1-3 h at 4 degrees C. The Scatchard analysis showed that there are approximately 20,000 receptors per cell with a single type of high affinity binding (Kd, 8 x 10(-10) M). The receptors for MDNCF are clearly distinct from the receptors for other cytokines and chemotactic agents, e.g., IL-1 alpha, TNF-alpha, and FMLP, C5a, leukotriene B4, and platelet activating factor. Based on the SDS-PAGE analysis of chemically crosslinked 125I-MDNCF receptor complex, there are two polypeptides that bind MDNCF; the molecular weight of these two MDNCF receptors were estimated to be 67,000 and 59,000. Treatment of a promyelocytic cell line, HL60, with 1.25% DMSO for 5 d in vitro increased the number of receptors up to 7,000 receptors\/cell with a Kd of 1.2 x 10(-9) M.<\/jats:p>","DOI":"10.1084\/jem.169.3.1185","type":"journal-article","created":{"date-parts":[[2004,6,24]],"date-time":"2004-06-24T07:56:10Z","timestamp":1088063770000},"page":"1185-1189","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":102,"title":["Identification and characterization of specific receptors for monocyte-derived neutrophil chemotactic factor (MDNCF) on human neutrophils."],"prefix":"10.1084","volume":"169","author":[{"given":"A K","family":"Samanta","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Immunoregulation, National Cancer Institute, Frederick, Maryland 21701."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J J","family":"Oppenheim","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Immunoregulation, National Cancer Institute, Frederick, Maryland 21701."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Matsushima","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Immunoregulation, National Cancer Institute, Frederick, Maryland 21701."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1989,3,1]]},"container-title":["The Journal of experimental medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/169\/3\/1185\/1669623\/1185.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/169\/3\/1185\/1669623\/1185.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T01:30:06Z","timestamp":1690248606000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/169\/3\/1185\/58013\/Identification-and-characterization-of-specific"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,3,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1989,3,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.169.3.1185","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,3,1]]}}}