{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T15:54:50Z","timestamp":1790006090781,"version":"4.0.1"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"3","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1989,3,1]]},"abstract":"<jats:p>Although follicle center cell (FCC) lymphomas represent mature B cells, a considerable percentage do not have detectable Ig production. We have used Southern blotting and the polymerase chain reaction (PCR) to study the involvement of translocations t(14;18) and t(8;14) in causing defective Ig production in 16 Ig- FCC-derived lymphomas and three Ig- B cell acute lymphoblastic leukemias. In 6 of 19 cases, a t(14;18) was present with the other allele either deleted or in germline. In two cases a t(14;18) and a t(8;14) affected both Ig alleles, as confirmed by karyotyping. In two other cases, rearrangement of both bcl-2 on chromosome 18 and c-myc on chromosome 8 were found as well. Although cytogenetic proof was not available, the latter was probably involved in t(8;14). Restriction map analysis of one more case showed rearrangement on the pseudo-JH3 gene on one allele and t(14;18) on the other. Thus, in 11 of 19 cases, defective Ig H chain production could be explained by the inactivation of both Ig H chain genes due to translocation of one allele, in combination with deletions or defective rearrangements of the other allele. In contrast, in 28 of 30 Ig+ lymphomas, one functional Ig H chain allele was found, either in, or not in, combination with t(14;18). In two cases a single rearranged Ig H chain allele was found in combination with rearrangement of bcl-2. No comigration of the single Ig rearrangement with bcl-2, however, was found both by Southern blotting and PCR, suggesting a variant bcl-2 translocation, which leaves the Ig H chain allele functionally intact.<\/jats:p>","DOI":"10.1084\/jem.169.3.613","type":"journal-article","created":{"date-parts":[[2004,6,24]],"date-time":"2004-06-24T07:56:10Z","timestamp":1088063770000},"page":"613-624","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":37,"title":["Translocation t(14;18) in B cell lymphomas as a cause for defective immunoglobulin production."],"prefix":"10.1084","volume":"169","author":[{"given":"D","family":"de Jong","sequence":"first","affiliation":[{"name":"Department of Pathology, University Medical Centre, Leiden, The Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B M","family":"Voetdijk","sequence":"additional","affiliation":[{"name":"Department of Pathology, University Medical Centre, Leiden, The Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G J","family":"Van Ommen","sequence":"additional","affiliation":[{"name":"Department of Pathology, University Medical Centre, Leiden, The Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J C","family":"Kluin-Nelemans","sequence":"additional","affiliation":[{"name":"Department of Pathology, University Medical Centre, Leiden, The Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G C","family":"Beverstock","sequence":"additional","affiliation":[{"name":"Department of Pathology, University Medical Centre, Leiden, The Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P M","family":"Kluin","sequence":"additional","affiliation":[{"name":"Department of Pathology, University Medical Centre, Leiden, The Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1989,3,1]]},"container-title":["The Journal of experimental medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/169\/3\/613\/1669681\/613.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/169\/3\/613\/1669681\/613.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T01:30:30Z","timestamp":1690248630000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/169\/3\/613\/58031\/Translocation-t-14-18-in-B-cell-lymphomas-as-a"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,3,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1989,3,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.169.3.613","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,3,1]]}}}