{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T17:24:43Z","timestamp":1770830683646,"version":"3.50.1"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"1","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1990,1,1]]},"abstract":"<jats:p>U937 cells attach to the RGDS-containing 80-kD fragment of fibronectin (Fn). The present report examined whether these cells recognize other domains of Fn. U937 cells attach to a 38-kD fragment derived from the A chain of Fn, which includes the Hep II domain and most of the alternatively spliced IIICS region. U937 did not bind to a 58-kD fragment derived from the B chain (which lacks IIICS) and has the Hep II site. They also did not bind to a 31-kD COOH-terminal fibrin-binding fragment or to a 29-kD fragment containing the Hep I domain. Cell adhesion to the 38-kD fragment was not inhibited by the 80-kD fragment, by GRGDSPC synthetic peptides, or by a mAb directed to the RGDS-containing domain of Fn. Attachment was completely inhibited by the 38-kD fragment and by the synthetic peptide CS-1, comprising the first 25 amino acid residues of IIICS. These results indicate that U937 cells interact with two sites of Fn, the RGDS-containing region, and the IIICS region.<\/jats:p>","DOI":"10.1084\/jem.171.1.351","type":"journal-article","created":{"date-parts":[[2004,6,24]],"date-time":"2004-06-24T07:56:10Z","timestamp":1088063770000},"page":"351-356","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":20,"title":["Specific binding of the human monocytic cell line U937 to the alternatively spliced connecting segment (IIICS) of fibronectin."],"prefix":"10.1084","volume":"171","author":[{"suffix":"Jr","given":"O C","family":"Ferreira","sequence":"first","affiliation":[{"name":"Lindsley F. Kimball Research Institute, New York Blood Center, New York 10021."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Garcia-Pardo","sequence":"additional","affiliation":[{"name":"Lindsley F. Kimball Research Institute, New York Blood Center, New York 10021."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Bianco","sequence":"additional","affiliation":[{"name":"Lindsley F. Kimball Research Institute, New York Blood Center, New York 10021."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1990,1,1]]},"container-title":["The Journal of experimental medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/171\/1\/351\/1670441\/351.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/171\/1\/351\/1670441\/351.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T01:38:02Z","timestamp":1690249082000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/171\/1\/351\/24224\/Specific-binding-of-the-human-monocytic-cell-line"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,1,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1990,1,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.171.1.351","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,1,1]]}}}