{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T18:08:45Z","timestamp":1783015725152,"version":"3.54.6"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"4","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1990,10,1]]},"abstract":"<jats:p>This study has identified a single amino acid change in the viral glycoprotein that profoundly affects the ability of lymphocytic choriomeningitis virus (LCMV) to persist in its natural host. Adult immunocompetent mice infected with a variant of the Armstrong strain, spleen isolate clone 13 (svA\/svA), harbor virus for several months and exhibit suppressed T cell responses. In contrast, adult mice infected with a reassortant virus (svA\/wtA) that contains the L segment of the spleen variant and the S segment of the parental wt Armstrong, make potent LCMV-specific CTL responses and clear the infection within 2-4 wk. These two viruses, spleen variant clone 13 and the reassortant svA\/wtA, are identical in their noncoding regions and show no amino acid changes in any of their viral genes except for one substitution in the glycoprotein. The reassortant virus svA\/wtA has a phenylalanine at amino acid residue 260 of the glycoprotein, whereas the spleen variant clone 13 has a leucine at this position. This study constitutes one of the first reports defining the genetic basis of viral persistence at the whole animal level, and identifying a single mutation that markedly increases the ability of a virus to persist in its natural host.<\/jats:p>","DOI":"10.1084\/jem.172.4.1043","type":"journal-article","created":{"date-parts":[[2004,6,24]],"date-time":"2004-06-24T07:56:10Z","timestamp":1088063770000},"page":"1043-1048","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":94,"title":["Genetic basis of viral persistence: single amino acid change in the viral glycoprotein affects ability of lymphocytic choriomeningitis virus to persist in adult mice."],"prefix":"10.1084","volume":"172","author":[{"given":"M","family":"Matloubian","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, UCLA School of Medicine 90024."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Somasundaram","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, UCLA School of Medicine 90024."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S R","family":"Kolhekar","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, UCLA School of Medicine 90024."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Selvakumar","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, UCLA School of Medicine 90024."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, UCLA School of Medicine 90024."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1990,10,1]]},"container-title":["The Journal of experimental medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/172\/4\/1043\/1671479\/1043.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/172\/4\/1043\/1671479\/1043.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T01:50:05Z","timestamp":1690249805000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/172\/4\/1043\/53788\/Genetic-basis-of-viral-persistence-single-amino"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,10,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1990,10,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.172.4.1043","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,10,1]]}}}