{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:29:27Z","timestamp":1791332967273,"version":"4.3.1"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"4","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1990,10,1]]},"abstract":"<jats:p>Coimplantation of small fragments of human fetal thymus and fetal liver into immunodeficient SCID mice resulted in the formation of a unique structure (Thy\/Liv). Thereafter, the SCID-hu mice showed reproducible and long-term reconstitution of human hematopoietic activity. For periods lasting 5-11 mo after transplantation, active T lymphopoiesis was observed inside the grafts and cells that were negative for T cell markers were found to have colony-forming units for granulocyte\/macrophage (CFU-GM) and erythroid burst-forming unit (BFU-E) activity in the methylcellulose colony assay. In addition, structures similar to normal human bone marrow were observed inside the Thy\/Liv grafts, consisting of blast cells, mature and immature forms of myelomonocytic cells, and megakaryocytes. These data indicate long-term maintenance, in vivo, of human progenitor cells for the T lymphoid, myelomonocytic, erythroid, and megakaryocytic lineages. The role of the implanted fetal liver fragments was analyzed using HLA-mismatched Thy\/Liv implants. The HLA type of the liver donor was found on T cells and macrophages in the graft. In addition, cells grown in the methylcellulose colony assay and cells in a bone marrow-like structure, the \"thymic isle,\" expressed the HLA type of the liver donor. Thus, the Thy\/Liv implants provided a microenvironment in which to follow human hematopoietic progenitor cells for multiple lineages. The formation of the Thy\/Liv structures also results in a continuous source of human T cells in the peripheral circulation of the SCID-hu mouse. Though present for 5-11 mo, these cells did not engage in a xenograft (graft-versus-host) reaction. This animal model, the first in which multilineage human hematopoietic activity is maintained for long periods of time, should be useful for the analysis of human hematopoiesis in vivo.<\/jats:p>","DOI":"10.1084\/jem.172.4.1055","type":"journal-article","created":{"date-parts":[[2004,6,24]],"date-time":"2004-06-24T07:56:10Z","timestamp":1088063770000},"page":"1055-1063","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":265,"title":["Long-term human hematopoiesis in the SCID-hu mouse."],"prefix":"10.1084","volume":"172","author":[{"given":"R","family":"Namikawa","sequence":"first","affiliation":[{"name":"SyStemix, Inc., Palo Alto, California 94303."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K N","family":"Weilbaecher","sequence":"additional","affiliation":[{"name":"SyStemix, Inc., Palo Alto, California 94303."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Kaneshima","sequence":"additional","affiliation":[{"name":"SyStemix, Inc., Palo Alto, California 94303."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E J","family":"Yee","sequence":"additional","affiliation":[{"name":"SyStemix, Inc., Palo Alto, California 94303."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J M","family":"McCune","sequence":"additional","affiliation":[{"name":"SyStemix, Inc., Palo Alto, California 94303."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1990,10,1]]},"container-title":["The Journal of experimental medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/172\/4\/1055\/1671528\/1055.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/172\/4\/1055\/1671528\/1055.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T01:50:34Z","timestamp":1690249834000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/172\/4\/1055\/53803\/Long-term-human-hematopoiesis-in-the-SCID-hu-mouse"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,10,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1990,10,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.172.4.1055","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,10,1]]}}}