{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T12:31:49Z","timestamp":1758630709652},"reference-count":0,"publisher":"Rockefeller University Press","issue":"2","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1991,2,1]]},"abstract":"<jats:p>The CD2 antigen is the target for an \"alternative\" T cell activation pathway. Numerous studies have demonstrated that pairs of monoclonal antibodies (mAbs) directed toward two different epitopes are required for activation of T cell receptor (TCR)-alpha\/beta + T cells via CD2. We have now explored the activation of human TCR-gamma\/delta + T cell clones by a panel of anti-CD2 mAbs directed against the sheep erythrocyte-binding (T11.1) epitope of CD2. Seven of seven gamma\/delta + clones expressing different molecular forms of the TCR-gamma\/delta responded to stimulation by a single anti-CD2 mAb (OKT11, 9E8, BW0110, M-T910) with IL-2 secretion and\/or proliferation. Immobilization of anti-CD2 mAbs in microculture plates was essential for activation of gamma\/delta + clones, which occurred in the absence of feeder cells. In addition to interleukin 2 (IL-2) production and proliferation, anti-CD2 mAbs also triggered cytotoxic effector activity in gamma\/delta + clones as measured against FcR+ P815 target cells. In contrast to gamma\/delta + clones (but in line with established data), none of five CD4+ or CD8+ TCR-alpha\/beta + clones were activated by any of the tested individual anti-CD2 mAbs. Taken together, our results reveal a striking difference between cloned gamma\/delta + and alpha\/beta + T cells in that gamma\/delta + T cells are selectively activated by a single anti-CD2 (T11.1) mAb, without need for the simultaneous signal of a second anti-CD2 mAb directed against another (T11.2 or T11.3) CD2 epitope.<\/jats:p>","DOI":"10.1084\/jem.173.2.297","type":"journal-article","created":{"date-parts":[[2004,6,24]],"date-time":"2004-06-24T07:56:10Z","timestamp":1088063770000},"page":"297-304","update-policy":"http:\/\/dx.doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":30,"title":["Selective activation of gamma\/delta + T cell clones by single anti-CD2 antibodies."],"prefix":"10.1084","volume":"173","author":[{"given":"S","family":"Wesselborg","sequence":"first","affiliation":[{"name":"Institut f\u00fcr Immunologie, Universit\u00e4t Heidelberg, Federal Republic of Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O","family":"Janssen","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Immunologie, Universit\u00e4t Heidelberg, Federal Republic of Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Pechhold","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Immunologie, Universit\u00e4t Heidelberg, Federal Republic of Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D","family":"Kabelitz","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Immunologie, Universit\u00e4t Heidelberg, Federal Republic of Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1991,2,1]]},"container-title":["The Journal of experimental medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/173\/2\/297\/1671811\/297.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/173\/2\/297\/1671811\/297.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T01:52:55Z","timestamp":1690249975000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/173\/2\/297\/24476\/Selective-activation-of-gamma-delta-T-cell-clones"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1991,2,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.173.2.297","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,2,1]]}}}