{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T09:14:40Z","timestamp":1790241280752,"version":"4.1.0"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"5","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1991,5,1]]},"abstract":"<jats:p>Severe deficiency of osteoclasts, monocytes, and peritoneal macrophages in osteopetrotic (op\/op) mutant mice is caused by the absence of functional macrophage colony-stimulating factor (M-CSF). To clarify the role of M-CSF in the osteoclast differentiation, we established a clonal stromal cell line OP6L7 capable of supporting hemopoiesis from newborn op\/op mouse calvaria. Although very few macrophages appeared in the cocultures of bone marrow cells and OP6L7 cells, a 50-fold larger number of macrophages was detected in the day 7 cocultures when purified recombinant human M-CSF (rhM-CSF) was exogenously supplied. Tartrate-resistant acid phosphatase (TRACP; a marker enzyme of osteoclasts)-positive cells appeared only when bone marrow cells were cultured in contact with OP6L7 cells and both rhM-CSF and 1 alpha, 25 (OH)2D3 were added. The TRACP-positive cells became multinucleated with increasing time in culture and expressed the c-fms\/M-CSF receptor. These results indicate that both contact with stromal cells and M-CSF are requisite for osteoclast differentiation under physiological conditions.<\/jats:p>","DOI":"10.1084\/jem.173.5.1291","type":"journal-article","created":{"date-parts":[[2004,6,24]],"date-time":"2004-06-24T07:56:10Z","timestamp":1088063770000},"page":"1291-1294","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":229,"title":["Essential role of macrophage colony-stimulating factor in the osteoclast differentiation supported by stromal cells."],"prefix":"10.1084","volume":"173","author":[{"given":"H","family":"Kodama","sequence":"first","affiliation":[{"name":"Department of Anatomy, Ohu University School of Dentistry, Koriyama, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Nose","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Ohu University School of Dentistry, Koriyama, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Niida","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Ohu University School of Dentistry, Koriyama, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Yamasaki","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Ohu University School of Dentistry, Koriyama, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1991,5,1]]},"container-title":["The Journal of experimental medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/173\/5\/1291\/1672121\/1291.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/173\/5\/1291\/1672121\/1291.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T01:56:16Z","timestamp":1690250176000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/173\/5\/1291\/24387\/Essential-role-of-macrophage-colony-stimulating"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1991,5,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.173.5.1291","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,5,1]]}}}