{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T17:11:26Z","timestamp":1791047486205,"version":"4.1.0"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"6","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1991,12,1]]},"abstract":"<jats:p>The immune response against lymphocytic choriomeningitis virus (LCMV) was studied in a mutant mouse strain that does not possess CD8+ T lymphocytes. Virus-specific cytotoxic T cell activity was generated in spleens of wild-type mice in an acute LCMV infection but was not measurable in mutant mice. Injection of replicating LCMV into footpads of wild-type mice induced a CD8+ T cell-mediated swelling that peaked on day 8, followed by a CD4+ T cell-mediated swelling that peaked on day 11, whereas mutant mice exhibited only the CD4+ T cell-mediated swelling. After intracerebral inoculation with LCMV-Armstrong, all wild-type mice died of classical CD8+ T cell-dependent choriomeningitis in 8-10 days. Mutant mice showed symptoms of general malaise but most of them survived. Mutant mice depleted of CD4+ T cells by monoclonal antibody treatment showed no clinical signs of sickness. On day 9 after intravenous infection with LCMV-WE, virus was detected at high titers in spleens and livers of mutant mice but not in those of wild-type mice. On day 70 after injection of LCMV-WE into footpads, virus was not detected in wild-type mice and in one of the three mutant mice tested, but was still measurable in kidneys of the other two mutant mice. These results confirm in a new animal model that CD8+ T cell-mediated immunity is crucial in LCMV clearance and in the immunopathological disease during LCMV infection. In addition, our results demonstrated a less severe form of choriomeningitis mediated by CD4+ T cells and slow clearance of LCMV by alternative pathways independent of CD8+ T cells.<\/jats:p>","DOI":"10.1084\/jem.174.6.1425","type":"journal-article","created":{"date-parts":[[2004,6,24]],"date-time":"2004-06-24T07:56:10Z","timestamp":1088063770000},"page":"1425-1429","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":150,"title":["Immune response against lymphocytic choriomeningitis virus infection in mice without CD8 expression."],"prefix":"10.1084","volume":"174","author":[{"given":"W P","family":"Fung-Leung","sequence":"first","affiliation":[{"name":"Ontario Cancer Institute, Toronto, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T M","family":"K\u00fcndig","sequence":"additional","affiliation":[{"name":"Ontario Cancer Institute, Toronto, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R M","family":"Zinkernagel","sequence":"additional","affiliation":[{"name":"Ontario Cancer Institute, Toronto, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T W","family":"Mak","sequence":"additional","affiliation":[{"name":"Ontario Cancer Institute, Toronto, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1991,12,1]]},"container-title":["The Journal of experimental medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/174\/6\/1425\/1672565\/1425.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/174\/6\/1425\/1672565\/1425.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T02:00:35Z","timestamp":1690250435000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/174\/6\/1425\/58164\/Immune-response-against-lymphocytic"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,12,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1991,12,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.174.6.1425","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,12,1]]}}}