{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T14:27:09Z","timestamp":1776263229801,"version":"3.50.1"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"2","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1992,2,1]]},"abstract":"<jats:p>Engagement of the CD3\/T cell antigen receptor complex on small, resting T cells is insufficient to trigger cell-mediated cytotoxicity or to induce a proliferative response. In the present study, we have used genetic transfection to demonstrate that interaction of the B7-BB1 B cell activation antigen with the CD28 T cell differentiation antigen costimulates cell-mediated cytotoxicity and proliferation initiated by either anti-CD2 or anti-CD3 monoclonal antibody (mAb). Moreover, a B7-negative Burkitt's lymphoma cell line that fails to stimulate an allogeneic mixed lymphocyte response is rendered a potent stimulator after transfection with B7. The mixed leukocyte reaction proliferative response against the B7 transfectant is inhibited by either anti-CD28 or B7 mAb. We also demonstrate that freshly isolated small, resting human T cells can mediate anti-CD3 or anti-CD2 mAb-redirected cytotoxicity against a murine Fc receptor-bearing mastocytoma transfected with human B7. These preexisting cytotoxic T lymphocytes in peripheral blood are present in both the CD4 and CD8 subsets, but are preferentially within the CD45RO+ \"memory\" population. While small, resting T cells apparently require costimulation by CD28\/B7 interactions, this requirement is lost after T cell activation. Anti-CD3 initiates a cytotoxic response mediated by in vitro cultured T cell clones in the absence of B7 ligand. The existence of functional cytolytic T cells in the small, resting T cell population may be advantageous in facilitating rapid responses to immune challenge.<\/jats:p>","DOI":"10.1084\/jem.175.2.353","type":"journal-article","created":{"date-parts":[[2004,6,24]],"date-time":"2004-06-24T07:56:10Z","timestamp":1088063770000},"page":"353-360","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":226,"title":["CD28 interaction with B7 costimulates primary allogeneic proliferative responses and cytotoxicity mediated by small, resting T lymphocytes."],"prefix":"10.1084","volume":"175","author":[{"given":"M","family":"Azuma","sequence":"first","affiliation":[{"name":"Department of Immunology, DNAX Research Institute for Molecular and Cellular Biology, Palo Alto, California 94304."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Cayabyab","sequence":"additional","affiliation":[{"name":"Department of Immunology, DNAX Research Institute for Molecular and Cellular Biology, Palo Alto, California 94304."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D","family":"Buck","sequence":"additional","affiliation":[{"name":"Department of Immunology, DNAX Research Institute for Molecular and Cellular Biology, Palo Alto, California 94304."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J H","family":"Phillips","sequence":"additional","affiliation":[{"name":"Department of Immunology, DNAX Research Institute for Molecular and Cellular Biology, Palo Alto, California 94304."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L L","family":"Lanier","sequence":"additional","affiliation":[{"name":"Department of Immunology, DNAX Research Institute for Molecular and Cellular Biology, Palo Alto, California 94304."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1992,2,1]]},"container-title":["The Journal of experimental medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/175\/2\/353\/1672644\/353.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/175\/2\/353\/1672644\/353.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T02:01:17Z","timestamp":1690250477000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/175\/2\/353\/50242\/CD28-interaction-with-B7-costimulates-primary"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1992,2,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.175.2.353","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,2,1]]}}}