{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T16:00:08Z","timestamp":1792598408026,"version":"4.5.0"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"5","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1992,5,1]]},"abstract":"<jats:p>Ly-1 B cells have the distinctive property of continuous self-replenishment and, as we have shown previously, can be further distinguished from conventional B cells on the basis of greatly elevated constitutive and inducible production of the recently described cytokine interleukin 10 (IL-10). To test the possibility that IL-10 acts as either an autocrine or paracrine growth factor for Ly-1 B cells, we treated mice continuously from birth to 8 wk of age with a monoclonal rat IgM antibody that specifically neutralizes mouse IL-10. Mice treated in this way lacked peritoneal-resident Ly-1 B cells, contained greatly reduced serum immunoglobulin M levels, and were unable to generate significant in vivo antibody responses to intraperitoneal injections of alpha 1,3-dextran or phosphorylcholine, antigens for which specific B cells reside in the Ly-1 B cell subset. In contrast, conventional splenic B cells of anti-IL-10-treated mice were normal with respect to total numbers, phenotype, and in vitro responsiveness to B cell mitogens and the thymus-dependent antigen trinitrophenyl-keyhole limpet hemocyanin (TNP-KLH). The mechanism of Ly-1 B cell depletion appeared to be related to elevation of endogenous interferon gamma (IFN-gamma) levels in anti-IL-10-treated mice, since coadministration of neutralizing anti-IFN-gamma antibodies substantially restored the number of peritoneal-resident Ly-1 B cells in these mice. These results implicate IL-10 as a regulator of Ly-1 B cell development, and identify a procedure to specifically deplete Ly-1 B cells, thereby allowing further evaluation of the role of these cells in the immune system.<\/jats:p>","DOI":"10.1084\/jem.175.5.1213","type":"journal-article","created":{"date-parts":[[2004,6,24]],"date-time":"2004-06-24T07:56:10Z","timestamp":1088063770000},"page":"1213-1220","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":139,"title":["Continuous anti-interleukin 10 antibody administration depletes mice of Ly-1 B cells but not conventional B cells."],"prefix":"10.1084","volume":"175","author":[{"given":"H","family":"Ishida","sequence":"first","affiliation":[{"name":"DNAX Research Institute, Palo Alto, California 94304."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Hastings","sequence":"additional","affiliation":[{"name":"DNAX Research Institute, Palo Alto, California 94304."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Kearney","sequence":"additional","affiliation":[{"name":"DNAX Research Institute, Palo Alto, California 94304."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Howard","sequence":"additional","affiliation":[{"name":"DNAX Research Institute, Palo Alto, California 94304."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1992,5,1]]},"container-title":["The Journal of experimental medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/175\/5\/1213\/1673347\/1213.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/175\/5\/1213\/1673347\/1213.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T04:44:30Z","timestamp":1690260270000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/175\/5\/1213\/58208\/Continuous-anti-interleukin-10-antibody"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1992,5,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.175.5.1213","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,5,1]]}}}