{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:09:46Z","timestamp":1790834986076,"version":"4.1.0"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"6","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1992,6,1]]},"abstract":"<jats:p>The immunologic mechanism of protective immunity to the intracellular parasite Listeria monocytogenes (Lm) is not well understood, however, antilisterial immunity can be adoptively transferred with T lymphocytes from Lm-immune donors. The Lm-immune cells are believed to produce macrophage-activating lymphokines, which leads to the eventual macrophage-dependent eradication of the bacterium. Increasing evidence suggests that immunity to Lm resides exclusively within the CD8+ T cell subset. It is possible that the Lm-immune CD8+ T cells function to release sequestered Lm from nonprofessional phagocytes to awaiting activated macrophage populations. This study was conducted to determine if listeriolysin O (LLO), which is an essential determinant of Lm pathogenicity, is also a target of the antilisterial immune response. We have found that target cells infected with a LLO+ Lm strain are lysed by Lm-immune cytotoxic cells, whereas target cells infected with a LLO- Lm mutant, or pulsed with a heat-killed Lm preparation, are not lysed by the Lm-immune effector cells. We have used a Bacillus subtilis (Bs) construct that expresses the LLO gene product and found that target cells infected with the LLO+ Bs construct are lysed by antilisterial cytotoxic cells. The antilisterial cytotoxic response is targeted against LLO, in that we have also used a Bs construct that expresses the perfringolysin (PLO) gene product and found that target cells infected with the PLO+ Bs are not lysed by antilisterial cytotoxic effector cells. These data strongly suggest that LLO is a target antigen of antilisterial immunity and may represent the dominant target during the expression of the immune response to Lm.<\/jats:p>","DOI":"10.1084\/jem.175.6.1467","type":"journal-article","created":{"date-parts":[[2004,6,24]],"date-time":"2004-06-24T07:56:10Z","timestamp":1088063770000},"page":"1467-1471","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":63,"title":["Listeriolysin O is a target of the immune response to Listeria monocytogenes."],"prefix":"10.1084","volume":"175","author":[{"given":"H G","family":"Bouwer","sequence":"first","affiliation":[{"name":"Earle A. Chiles Research Institute, Providence Medical Center, Portland, Oregon 97213."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C S","family":"Nelson","sequence":"additional","affiliation":[{"name":"Earle A. Chiles Research Institute, Providence Medical Center, Portland, Oregon 97213."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B L","family":"Gibbins","sequence":"additional","affiliation":[{"name":"Earle A. Chiles Research Institute, Providence Medical Center, Portland, Oregon 97213."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D A","family":"Portnoy","sequence":"additional","affiliation":[{"name":"Earle A. Chiles Research Institute, Providence Medical Center, Portland, Oregon 97213."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D J","family":"Hinrichs","sequence":"additional","affiliation":[{"name":"Earle A. Chiles Research Institute, Providence Medical Center, Portland, Oregon 97213."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1992,6,1]]},"container-title":["The Journal of experimental medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/175\/6\/1467\/1673211\/1467.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/175\/6\/1467\/1673211\/1467.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T04:43:09Z","timestamp":1690260189000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/175\/6\/1467\/57441\/Listeriolysin-O-is-a-target-of-the-immune-response"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,6,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1992,6,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.175.6.1467","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,6,1]]}}}