{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T10:16:12Z","timestamp":1770718572342,"version":"3.49.0"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"3","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1992,9,1]]},"abstract":"<jats:p>Disease activity in systemic lupus erythematosus is closely associated with the appearance of immunoglobulin (Ig)G antibody to native DNA in both humans and mice. Like normal antibody responses, the anti-DNA autoantibody first appears as IgM and then switches to IgG. Structural studies of IgG anti-DNA suggest that these antibodies are the products of clonally selected, specifically stimulated B cells. The origins of the IgM anti-DNA have been less clear. To determine whether the earlier appearing IgM anti-DNA antibody in autoimmune mice also derives from clonally selected, specifically stimulated B cells or B cells activated by nonselective, polyclonal stimuli, we have analyzed the molecular and serological characteristics of a large number of monoclonal IgM anti-DNA antibodies from autoimmune (NZB x NZW)F1 mice. We have also analyzed IgM and IgG anti-DNA hybridomas obtained from the same individual mice to determine how the later-appearing IgG autoantibody may be related to the earlier-appearing IgM autoantibody within an individual mouse. The results demonstrate that: (a) IgM anti-DNA, like IgG, has the characteristics of a specifically stimulated antibody; (b) IgM and IgG anti-DNA antibodies have similar variable region structures and within individual mice may be produced by B cells derived from the same clonal precursors; (c) recurrent germline and somatically derived VH and VL structures may influence the specificity of anti-DNA monoclonal antibody for denatured vs. native DNA; and (d) the results provide a structural explanation for the selective development of IgG antibody to native DNA as autoimmunity to DNA progresses in (NZB x NZW)F1 mice.<\/jats:p>","DOI":"10.1084\/jem.176.3.761","type":"journal-article","created":{"date-parts":[[2004,6,24]],"date-time":"2004-06-24T07:56:10Z","timestamp":1088063770000},"page":"761-779","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":175,"title":["Both IgM and IgG anti-DNA antibodies are the products of clonally selective B cell stimulation in (NZB x NZW)F1 mice."],"prefix":"10.1084","volume":"176","author":[{"given":"D M","family":"Tillman","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of Tennessee, Memphis 38163."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N T","family":"Jou","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Tennessee, Memphis 38163."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R J","family":"Hill","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Tennessee, Memphis 38163."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T N","family":"Marion","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Tennessee, Memphis 38163."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1992,9,1]]},"container-title":["The Journal of experimental medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/176\/3\/761\/1838975\/761.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/176\/3\/761\/1838975\/761.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,28]],"date-time":"2023-07-28T19:47:03Z","timestamp":1690573623000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/176\/3\/761\/24615\/Both-IgM-and-IgG-anti-DNA-antibodies-are-the"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,9,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1992,9,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.176.3.761","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,9,1]]}}}