{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T21:19:32Z","timestamp":1791148772605,"version":"4.1.0"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"5","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1992,11,1]]},"abstract":"<jats:p>Pre-B cell lines proliferating for several months on stromal cells in the presence of interleukin 7 (IL-7) were established from fetal liver of (NZB x NZW)F1 mice. They express the B lineage-specific markers PB76, B220, and VpreB, but do not express surface immunoglobulin (sIg). Upon removal of IL-7 from the culture, they differentiate to sIg+ B cells that can then be stimulated by lipopolysaccharide to become IgM-secreting cells. Transfer of these pre-B cell lines into SCID mice leads to hypergammaglobulinemia of IgM (600-900 micrograms\/ml), IgG2a (1-3 mg\/ml), and IgG3 (300-500 micrograms\/ml) for the next 3-5 mo. The spleen appears populated with (NZB x NZW)F1-derived pre-B cells, few B cells, and many IgM and\/or IgG-producing plasma cells. In contrast, SCID mice populated with pre-B cell lines of normal (C57BL\/6 x DBA\/2)F1 mouse fetal liver develop normal levels of serum IgM (approximately 100-300 micrograms\/ml), almost no detectable levels of IgG, and no plasma cell hyperplasia. The (NZB x NZW)F1 pre-B cell-populated SCID mice contain elevated serum titers of IgG antinuclear autoantibodies, but no retroviral gp70-specific nor erythrocyte-specific autoantibodies. Up to 20% of the SCID mice develop proteinuria as a consequence of IgG deposits in the kidney glomeruli during a 7-mo period of observation. All signs of autoimmune disease seen in these mice are independent of the sex of the SCID host. This experimental system provides a distinction between the disease-determining (NZB x NZW)F1 genes, which are expressed in the B lymphocyte lineage and cause the development of the disease, from those expressed in other cell lineages which only modulate its progression.<\/jats:p>","DOI":"10.1084\/jem.176.5.1343","type":"journal-article","created":{"date-parts":[[2004,6,24]],"date-time":"2004-06-24T07:56:10Z","timestamp":1088063770000},"page":"1343-1353","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":96,"title":["Development of autoimmune disease in SCID mice populated with long-term \"in vitro\" proliferating (NZB x NZW)F1 pre-B cells."],"prefix":"10.1084","volume":"176","author":[{"given":"L","family":"Reininger","sequence":"first","affiliation":[{"name":"Basel Institute for Immunology, Switzerland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Radaszkiewicz","sequence":"additional","affiliation":[{"name":"Basel Institute for Immunology, Switzerland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Kosco","sequence":"additional","affiliation":[{"name":"Basel Institute for Immunology, Switzerland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F","family":"Melchers","sequence":"additional","affiliation":[{"name":"Basel Institute for Immunology, Switzerland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A G","family":"Rolink","sequence":"additional","affiliation":[{"name":"Basel Institute for Immunology, Switzerland."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1992,11,1]]},"container-title":["The Journal of experimental medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/176\/5\/1343\/1673489\/1343.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/176\/5\/1343\/1673489\/1343.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T04:45:44Z","timestamp":1690260344000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/176\/5\/1343\/58246\/Development-of-autoimmune-disease-in-SCID-mice"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,11,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1992,11,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.176.5.1343","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,11,1]]}}}