{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T11:46:35Z","timestamp":1792669595617,"version":"4.5.0"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"6","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1992,12,1]]},"abstract":"<jats:p>Signaling through the cell surface molecule, CD40, is known to play an important role in the proliferation and differentiation of B lymphocytes. Using the thymoma cell line EL4, we recently identified and cloned a cDNA encoding a murine ligand for the CD40 molecule (mCD40-L) and showed that it has biological activity in vitro. A cDNA encoding a human homologue of the mCD40-L was isolated using crosshybridization techniques from an activated peripheral blood T cell library. The predicted amino acid sequence indicates that this human ligand for CD40 (hCD40-L) is a 261 amino acid type II membrane protein that exhibits 78% amino acid identity with its murine counterpart. Northern blot and FACS analyses suggest that the hCD40-L is restricted in its expression to T lymphocytes, and that it is most abundant on the CD4+ T cell subpopulation. Cells transfected with hCD40-L caused the proliferation of human tonsil B cells in the absence of costimuli and, in the presence of interleukin 4, induced immunoglobulin E secretion from purified human B cells. A comparison of the efficacy of the hCD40-L and mCD40-L in these assays is presented.<\/jats:p>","DOI":"10.1084\/jem.176.6.1543","type":"journal-article","created":{"date-parts":[[2004,6,24]],"date-time":"2004-06-24T07:56:10Z","timestamp":1088063770000},"page":"1543-1550","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":280,"title":["Recombinant human CD40 ligand stimulates B cell proliferation and immunoglobulin E secretion."],"prefix":"10.1084","volume":"176","author":[{"given":"M K","family":"Spriggs","sequence":"first","affiliation":[{"name":"Department of Molecular Biology, Immunex Research and Development Corporation, Seattle, Washington 98101."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R J","family":"Armitage","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Immunex Research and Development Corporation, Seattle, Washington 98101."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Strockbine","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Immunex Research and Development Corporation, Seattle, Washington 98101."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K N","family":"Clifford","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Immunex Research and Development Corporation, Seattle, Washington 98101."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B M","family":"Macduff","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Immunex Research and Development Corporation, Seattle, Washington 98101."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T A","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Immunex Research and Development Corporation, Seattle, Washington 98101."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C R","family":"Maliszewski","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Immunex Research and Development Corporation, Seattle, Washington 98101."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W C","family":"Fanslow","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Immunex Research and Development Corporation, Seattle, Washington 98101."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1992,12,1]]},"container-title":["The Journal of experimental medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/176\/6\/1543\/1673797\/1543.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/176\/6\/1543\/1673797\/1543.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T04:48:13Z","timestamp":1690260493000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/176\/6\/1543\/24748\/Recombinant-human-CD40-ligand-stimulates-B-cell"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,12,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1992,12,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.176.6.1543","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,12,1]]}}}