{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T05:57:34Z","timestamp":1779343054970,"version":"3.51.4"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"2","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1993,2,1]]},"abstract":"<jats:p>Using an in vivo test system, the role of the beta 1 integrin very late activation antigen-4 (VLA-4) in eosinophil accumulation in allergic and nonallergic inflammatory reactions was investigated. Eosinophil infiltration and edema formation were measured as the local accumulation of intravenously injected 111In-labeled eosinophils and 125I-human serum albumin. The inflammatory reactions investigated were a passive cutaneous anaphylaxis (PCA) reaction and responses elicited by intradermal soluble inflammatory mediators (platelet-activating factor, leukotriene B4, C5a des Arg), arachidonic acid, and zymosan particles. The in vitro pretreatment of 111In-eosinophils with the anti-VLA-4 monoclonal antibody (mAb) HP1\/2, which crossreacts with guinea pig eosinophils, suppressed eosinophil accumulation in all the inflammatory reactions investigated. Eosinophil accumulation was inhibited to the same extent when mAb HP1\/2 was administered intravenously. It is interesting that HP1\/2 had no effect on stimulated edema formation. These results suggest a role for VLA-4 in eosinophil accumulation in vivo and indicate a dissociation between the inflammatory events of eosinophil accumulation and edema formation.<\/jats:p>","DOI":"10.1084\/jem.177.2.561","type":"journal-article","created":{"date-parts":[[2004,6,24]],"date-time":"2004-06-24T07:56:10Z","timestamp":1088063770000},"page":"561-566","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":125,"title":["A monoclonal antibody recognizing very late activation antigen-4 inhibits eosinophil accumulation in vivo."],"prefix":"10.1084","volume":"177","author":[{"given":"V B","family":"Weg","sequence":"first","affiliation":[{"name":"Department of Applied Pharmacology, National Heart and Lung Institute, London, UK."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T J","family":"Williams","sequence":"additional","affiliation":[{"name":"Department of Applied Pharmacology, National Heart and Lung Institute, London, UK."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R R","family":"Lobb","sequence":"additional","affiliation":[{"name":"Department of Applied Pharmacology, National Heart and Lung Institute, London, UK."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Nourshargh","sequence":"additional","affiliation":[{"name":"Department of Applied Pharmacology, National Heart and Lung Institute, London, UK."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1993,2,1]]},"container-title":["The Journal of experimental medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/177\/2\/561\/1674203\/561.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/177\/2\/561\/1674203\/561.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T04:51:59Z","timestamp":1690260719000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/177\/2\/561\/50621\/A-monoclonal-antibody-recognizing-very-late"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1993,2,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.177.2.561","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,2,1]]}}}