{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T10:16:09Z","timestamp":1789985769638,"version":"4.0.1"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"6","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1993,6,1]]},"abstract":"<jats:p>The frequencies of human T cell lymphotropic virus type 1 (HTLV-1)-specific CD8+ precursor cytotoxic T lymphocytes (pCTL) were quantitated from lymphocytes obtained from the peripheral blood and cerebrospinal fluid (CSF) of infected individuals with and without HTLV-1-associated neurological disease. An estimate of the pCTL was obtained by separating CD8+ cells, plating these cells in limiting dilution, and testing wells for HTLV-1 specific lysis. Targets consisted of autologous lymphoblastoid cell lines (LCL) infected with vaccinia constructs expressing HTLV-1 gene products or LCL pulsed with HTLV-1 synthetic peptides. In patients with HTLV-1-associated myelopathy\/tropical spastic paraparesis (HAM\/TSP), the frequency of HTLV-1 p40X-specific pCTL was at least 40-280-fold higher than in asymptomatic HTLV-1-infected individuals. All HAM\/TSP patients (five of five) predominantly recognized HTLV-1 products encoded within the pX region. Lower pCTL to env were demonstrated in three patients, and only one of five HAM\/TSP patients had pCTL to gag. A synthetic peptide corresponding to the tax region of HTLV-1 (peptide 11-19, amino acid sequence LLFGYPVYV) was recognized in association with human histocompatibility leukocyte antigen (HLA)-A2 in two HLA-A2 HAM\/TSP patients with a high CD8+ pCTL frequency of 1\/325 and 1\/265, respectively. A second immunodominant region of HTLV-1 tax (peptide 90-55, amino acid sequence VPYKRIEEL) was identified to be restricted by HLA-B14 in two HLA-B14 HAM\/TSP patients with a CD8+ pCTL frequency of 1\/640 and 1\/1,125, respectively. Lymphocytes from the CSF of a patient with HAM\/TSP also showed a pCTL frequency against p40X of similar magnitude to that demonstrated from peripheral blood lymphocytes (PBL). The HLA-A2-mediated CSF pCTL activity to the immunodominant tax-specific peptide 11-19 was also comparable to pCTL from PBL. These results indicate that an extremely high pCTL frequency to HTLV-1 tax-encoded peptides may be related to pathogenesis of myeloneuropathy associated with HTLV-1.<\/jats:p>","DOI":"10.1084\/jem.177.6.1567","type":"journal-article","created":{"date-parts":[[2004,6,24]],"date-time":"2004-06-24T07:56:10Z","timestamp":1088063770000},"page":"1567-1573","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":148,"title":["High human T cell lymphotropic virus type 1 (HTLV-1)-specific precursor cytotoxic T lymphocyte frequencies in patients with HTLV-1-associated neurological disease."],"prefix":"10.1084","volume":"177","author":[{"given":"I","family":"Elovaara","sequence":"first","affiliation":[{"name":"Neuroimmunology Branch, National Institutes of Health, Bethesda, Maryland 20892."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Koenig","sequence":"additional","affiliation":[{"name":"Neuroimmunology Branch, National Institutes of Health, Bethesda, Maryland 20892."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A Y","family":"Brewah","sequence":"additional","affiliation":[{"name":"Neuroimmunology Branch, National Institutes of Health, Bethesda, Maryland 20892."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R M","family":"Woods","sequence":"additional","affiliation":[{"name":"Neuroimmunology Branch, National Institutes of Health, Bethesda, Maryland 20892."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Lehky","sequence":"additional","affiliation":[{"name":"Neuroimmunology Branch, National Institutes of Health, Bethesda, Maryland 20892."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Jacobson","sequence":"additional","affiliation":[{"name":"Neuroimmunology Branch, National Institutes of Health, Bethesda, Maryland 20892."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1993,6,1]]},"container-title":["The Journal of experimental medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/177\/6\/1567\/1674496\/1567.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/177\/6\/1567\/1674496\/1567.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T04:57:52Z","timestamp":1690261072000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/177\/6\/1567\/50424\/High-human-T-cell-lymphotropic-virus-type-1-HTLV-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,6,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1993,6,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.177.6.1567","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,6,1]]}}}