{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T15:40:58Z","timestamp":1791042058021,"version":"4.1.0"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"2","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1993,8,1]]},"abstract":"<jats:p>The bcl-2 gene product has been shown to prevent apoptotic cell death. We have now investigated the bcl-2 protein expression by resting and activated mature T cell populations. Freshly isolated CD45RO+ T cells within CD4+ and CD8+ subsets expressed significantly less bcl-2 than CD45RO- (CD45RA+) T cells (p &amp;lt; 0.001). When CD45RA+ T cells within both CD4+ and CD8+ subsets were activated in vitro, the transition to CD45RO phenotype was associated with a decrease in bcl-2 expression. Patients with acute viral infections such as infectious mononucleosis caused by Epstein-Barr virus infections or chickenpox, resulting from varicella zoster virus infection, had circulating populations of activated CD45RO+ T cells which also showed low bcl-2 expression. In these patients, a significant correlation was seen between low bcl-2 expression by activated T cells and their apoptosis in culture (r = 0.94, p &amp;lt; 0.001). These results suggest that the primary activation of T cells leads to the expansion of a population that is destined to perish unless rescued by some extrinsic event. Thus the suicide of CD45RO+ T cells could be prevented by the addition of interleukin 2 to the culture medium which resulted in a concomitant increase in the bcl-2 expression of these cells. Alternatively, apoptosis was also prevented by coculturing the activated T lymphocytes with fibroblasts, which maintained the viability of lymphoid cells in a restinglike state but with low bcl-2 expression. The paradox that the CD45RO+ population contains the primed\/memory T cell pool yet expresses low bcl-2 and is susceptible to apoptosis can be reconciled by the observations that maintenance of T cell memory may be dependent on the continuous restimulation of T cells, which increases their bcl-2 expression. Furthermore, the propensity of CD45RO+ T cells to extravasate may facilitate encounter with fibroblast-like cells in tissue stroma and thus be an important additional factor which promotes the survival of selected primed\/memory T cells in vivo.<\/jats:p>","DOI":"10.1084\/jem.178.2.427","type":"journal-article","created":{"date-parts":[[2004,6,24]],"date-time":"2004-06-24T07:56:10Z","timestamp":1088063770000},"page":"427-438","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":238,"title":["The significance of low bcl-2 expression by CD45RO T cells in normal individuals and patients with acute viral infections. The role of apoptosis in T cell memory."],"prefix":"10.1084","volume":"178","author":[{"given":"A N","family":"Akbar","sequence":"first","affiliation":[{"name":"Department of Clinical Immunology, Royal Free Hospital School of Medicine, London, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N","family":"Borthwick","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology, Royal Free Hospital School of Medicine, London, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Salmon","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology, Royal Free Hospital School of Medicine, London, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W","family":"Gombert","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology, Royal Free Hospital School of Medicine, London, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Bofill","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology, Royal Free Hospital School of Medicine, London, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N","family":"Shamsadeen","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology, Royal Free Hospital School of Medicine, London, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Pilling","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology, Royal Free Hospital School of Medicine, London, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Pett","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology, Royal Free Hospital School of Medicine, London, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J E","family":"Grundy","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology, Royal Free Hospital School of Medicine, London, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G","family":"Janossy","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology, Royal Free Hospital School of Medicine, London, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1993,8,1]]},"container-title":["The Journal of experimental medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/178\/2\/427\/1674987\/427.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/178\/2\/427\/1674987\/427.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T05:04:37Z","timestamp":1690261477000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/178\/2\/427\/50272\/The-significance-of-low-bcl-2-expression-by-CD45RO"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,8,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1993,8,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.178.2.427","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,8,1]]}}}