{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T04:58:31Z","timestamp":1791089911163,"version":"4.1.0"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"2","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1993,8,1]]},"abstract":"<jats:p>The partial sequence of a gene encoding the COOH terminus of a protein of apparent molecular weight of 160 kD associated with the flagellum of trypomastigotes of Trypanosoma cruzi (FL-160 now renamed to FL-160-1) has been previously reported. The COOH terminus of FL-160-1 has an epitope, defined by 12 amino acids, which molecularly miMics a nervous tissue antigen of 48 kD found in myenteric plexus, sciatic nerve, and a subset of cells in the central nervous system. We now report that FL-160 is a family of highly related genes. The sequence has been determined for the entire open reading frame (ORF) of one of the members of the FL-160 gene family (FL-160-2) and three other partial ORFs. Sequence analysis reveals the various members of the FL-160 gene family to be approximately 80% homologous in the predicted amino acid sequence, but all retain the 12-amino acid molecular mimicry epitope on the COOH terminus. Comparison of the sequence of FL-160-2 to other sequences demonstrates amino acid homology to bacterial sialidase (27%), members of the SA85 gene family (25-30%) and the shed acute-phase antigen\/neuraminidase\/trans-sialidase gene family (25-30%). Quantitative hybridization at high stringency suggests 750 copies of FL-160 are present in the DNA of each parasite. Reverse transcription and sequence analysis demonstrates that at least five of the members of the FL-160 gene family are transcribed. The NH2 terminus of one of the FL-160 gene products was expressed and antibodies prepared. Antibodies directed to either the COOH or the NH2 terminus of FL-160 bind a 160-kD T. cruzi protein. Both antibodies bind the surface membrane in the flagellar pocket of the trypomastigote. Antibodies to the NH2 terminus bind epineurium and scattered linear densities in sciatic nerve in a pattern distinct from the pattern with antibodies to the COOH terminus. Thus, there are at least two distinct molecular mimicry epitopes on the FL-160 molecule and both mimic epitopes found in nervous tissues. FL-160 may be involved in the generation of autoimmunity to nervous tissues by molecular mimicry, observed in chronic Chagas' disease.<\/jats:p>","DOI":"10.1084\/jem.178.2.681","type":"journal-article","created":{"date-parts":[[2004,6,24]],"date-time":"2004-06-24T07:56:10Z","timestamp":1088063770000},"page":"681-694","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":47,"title":["FL-160 proteins of Trypanosoma cruzi are expressed from a multigene family and contain two distinct epitopes that mimic nervous tissues."],"prefix":"10.1084","volume":"178","author":[{"given":"W C","family":"Van Voorhis","sequence":"first","affiliation":[{"name":"Department of Medicine, School of Medicine, University of Washington, Seattle 98195."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Barrett","sequence":"additional","affiliation":[{"name":"Department of Medicine, School of Medicine, University of Washington, Seattle 98195."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Koelling","sequence":"additional","affiliation":[{"name":"Department of Medicine, School of Medicine, University of Washington, Seattle 98195."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A G","family":"Farr","sequence":"additional","affiliation":[{"name":"Department of Medicine, School of Medicine, University of Washington, Seattle 98195."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1993,8,1]]},"container-title":["The Journal of experimental medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/178\/2\/681\/1674597\/681.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/178\/2\/681\/1674597\/681.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T04:59:20Z","timestamp":1690261160000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/178\/2\/681\/50253\/FL-160-proteins-of-Trypanosoma-cruzi-are-expressed"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,8,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1993,8,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.178.2.681","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,8,1]]}}}