{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T21:33:02Z","timestamp":1791063182212,"version":"4.1.0"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"2","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1994,2,1]]},"abstract":"<jats:p>To determine whether T cells, like B cells, can become clonally expanded in normal individuals as a function of age, we compared the T cell V beta repertoire of cord blood to that of peripheral blood from normal donors over 65 yr of age. T cells from elderly subjects contained expanded subsets (greater than the mean+three standard deviations) of T cell receptor (TCR) V beta populations. These expanded subsets were observed primarily among CD8, but not CD4 cells, represented up to 37.5% of all CD8 cells, and were present in most elderly subjects. An expanded V beta 5.2\/3 CD8 subset and a V beta 6.7a CD8 subset from separate donors were analyzed by reverse transcriptase-polymerase chain reaction, cloning and sequencing of the TCR beta chain VDJ junction. In both cases the expanded subsets were mono- or oligoclonal while control CD4 populations were polyclonal. Using two-color flow cytometry it was possible to identify the expanded V beta 6.7a subset as CD8+ CD28-CD11b+ cells. In three of five random old subjects similar expansions of V beta subsets were found specifically in the CD8+ CD28- subpopulation, an interesting subset of cytotoxic T lymphocytes, known to lack proliferative responses to TCR stimuli. It is common practice to use the demonstration of clonality as a diagnostic indicator for T cell lymphoma\/leukemia. In view of the high frequency of expanded T clones of T cells in normal elderly subjects the diagnostic usefulness of this test should be reexamined.<\/jats:p>","DOI":"10.1084\/jem.179.2.609","type":"journal-article","created":{"date-parts":[[2004,6,24]],"date-time":"2004-06-24T07:56:10Z","timestamp":1088063770000},"page":"609-618","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":582,"title":["Clonal populations of T cells in normal elderly humans: the T cell equivalent to \"benign monoclonal gammapathy\"."],"prefix":"10.1084","volume":"179","author":[{"given":"D N","family":"Posnett","sequence":"first","affiliation":[{"name":"Department of Medicine, Cornell University Medical College, New York 10021."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Sinha","sequence":"additional","affiliation":[{"name":"Department of Medicine, Cornell University Medical College, New York 10021."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Kabak","sequence":"additional","affiliation":[{"name":"Department of Medicine, Cornell University Medical College, New York 10021."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Russo","sequence":"additional","affiliation":[{"name":"Department of Medicine, Cornell University Medical College, New York 10021."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1994,2,1]]},"container-title":["The Journal of experimental medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/179\/2\/609\/1675560\/609.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/179\/2\/609\/1675560\/609.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T05:55:20Z","timestamp":1690264520000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/179\/2\/609\/57553\/Clonal-populations-of-T-cells-in-normal-elderly"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1994,2,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.179.2.609","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,2,1]]}}}