{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T05:33:29Z","timestamp":1790400809191,"version":"4.1.0"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"5","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1994,5,1]]},"abstract":"<jats:p>The gram negative, microaerophilic bacterium Helicobacter pylori colonizes the human gastric mucosa and establishes a chronic infection that is tightly associated with atrophic gastritis, peptic ulcer, and gastric carcinoma. Cloning of the H. pylori cytotoxin gene shows that the protein is synthesized as a 140-kD precursor that is processed to a 94-kD fully active toxin. Oral administration to mice of the purified 94-kD protein caused ulceration and gastric lesions that bear some similarities to the pathology observed in humans. The cloning of the cytotoxin gene and the development of a mouse model of human gastric disease will provide the basis for the understanding of H. pylori pathogenesis and the development of therapeutics and vaccines.<\/jats:p>","DOI":"10.1084\/jem.179.5.1653","type":"journal-article","created":{"date-parts":[[2004,6,24]],"date-time":"2004-06-24T07:56:10Z","timestamp":1088063770000},"page":"1653-1658","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":476,"title":["Gene structure of the Helicobacter pylori cytotoxin and evidence of its key role in gastric disease."],"prefix":"10.1084","volume":"179","author":[{"given":"J L","family":"Telford","sequence":"first","affiliation":[{"name":"Department of Molecular Biology, Immunobiological Research Institute Siena, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"Ghiara","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Immunobiological Research Institute Siena, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Dell'Orco","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Immunobiological Research Institute Siena, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Comanducci","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Immunobiological Research Institute Siena, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Burroni","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Immunobiological Research Institute Siena, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Bugnoli","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Immunobiological Research Institute Siena, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M F","family":"Tecce","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Immunobiological Research Institute Siena, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Censini","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Immunobiological Research Institute Siena, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Covacci","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Immunobiological Research Institute Siena, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z","family":"Xiang","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Immunobiological Research Institute Siena, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1994,5,1]]},"container-title":["The Journal of experimental medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/179\/5\/1653\/1675530\/1653.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/179\/5\/1653\/1675530\/1653.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T05:54:55Z","timestamp":1690264495000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/179\/5\/1653\/25075\/Gene-structure-of-the-Helicobacter-pylori"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1994,5,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.179.5.1653","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,5,1]]}}}