{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T19:18:51Z","timestamp":1783624731536,"version":"3.55.0"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"5","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1994,11,1]]},"abstract":"<jats:p>CD43 (leukosialin, gpL115, sialophorin) is a major sialoglycoprotein widely expressed on hematopoietic cells that is defective in the congenital immunodeficiency Wiskott-Aldrich syndrome. It is thought to play an important role in cell-cell interactions and to be a costimulatory molecule for T lymphocyte activation. Using a metabolic 35SO4(2-) radiolabeling assay or biotinylation of cell surface proteins, we describe here that CD43 are sulfated molecules the glycosylation of which is altered in human immunodeficiency virus type 1 (HIV-1)-infected leukemic T cells of the CEM line. Hyposialylation of O-glycans and changed substitution on N-acetylgalactosamine residues are observed. The glycosylation defect is associated with an impairment of CD43-mediated homotypic aggregation which can be restored by resialylation. The hyposialylation of CD43 on HIV-1+ cells may explain the high prevalence of autoantibodies directed against nonsialylated CD43 that have been detected in HIV-1-infected individuals. A defect in glycosylation of important molecules such as CD43 or, as we recently described, CD45 may explain alterations of T cell functions and viability in HIV-1-infected individuals. In addition, a possible implication of hyposialylation in the HIV-1-infected cells entrapment in lymph nodes could be envisioned.<\/jats:p>","DOI":"10.1084\/jem.180.5.1609","type":"journal-article","created":{"date-parts":[[2004,6,24]],"date-time":"2004-06-24T07:56:10Z","timestamp":1088063770000},"page":"1609-1617","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":46,"title":["Altered glycosylation of leukosialin, CD43, in HIV-1-infected cells of the CEM line."],"prefix":"10.1084","volume":"180","author":[{"given":"J C","family":"Lefebvre","sequence":"first","affiliation":[{"name":"Laboratoire de Virologie, Facult\u00e9 de M\u00e9decine, H\u00f4pital de Cimiez, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V","family":"Giordanengo","sequence":"additional","affiliation":[{"name":"Laboratoire de Virologie, Facult\u00e9 de M\u00e9decine, H\u00f4pital de Cimiez, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Limouse","sequence":"additional","affiliation":[{"name":"Laboratoire de Virologie, Facult\u00e9 de M\u00e9decine, H\u00f4pital de Cimiez, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Doglio","sequence":"additional","affiliation":[{"name":"Laboratoire de Virologie, Facult\u00e9 de M\u00e9decine, H\u00f4pital de Cimiez, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Cucchiarini","sequence":"additional","affiliation":[{"name":"Laboratoire de Virologie, Facult\u00e9 de M\u00e9decine, H\u00f4pital de Cimiez, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F","family":"Monpoux","sequence":"additional","affiliation":[{"name":"Laboratoire de Virologie, Facult\u00e9 de M\u00e9decine, H\u00f4pital de Cimiez, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Mariani","sequence":"additional","affiliation":[{"name":"Laboratoire de Virologie, Facult\u00e9 de M\u00e9decine, H\u00f4pital de Cimiez, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J F","family":"Peyron","sequence":"additional","affiliation":[{"name":"Laboratoire de Virologie, Facult\u00e9 de M\u00e9decine, H\u00f4pital de Cimiez, France."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1994,11,1]]},"container-title":["The Journal of experimental medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/180\/5\/1609\/1676993\/1609.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/180\/5\/1609\/1676993\/1609.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T06:43:08Z","timestamp":1690267388000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/180\/5\/1609\/25513\/Altered-glycosylation-of-leukosialin-CD43-in-HIV-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,11,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1994,11,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.180.5.1609","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,11,1]]}}}