{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T17:33:10Z","timestamp":1790875990095,"version":"4.1.0"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"5","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1995,11,1]]},"abstract":"<jats:p>The Apo-1\/Fas antigen (CD95) mediates programmed cell death of lymphocytes when bound by Fas ligand or anti-Apo-1\/Fas antibody. In contrast, the CD40 antigen provides a potent activation and survival signal to B lymphocytes when it is engaged by its T cell ligand (CD40L, gp39) or cross-linked by anti-CD40 antibody. In this study, we use human tonsillar B cells and the Ramos Burkitt's lymphoma B cell line, which serves as a model for human germinal center B lymphocytes, to study the effectors of Apo-1\/Fas expression and apoptosis of human B cells. We found that Apo-1\/Fas expression was upregulated on both malignant and normal human B lymphocytes after CD40 ligation induced by (a) cognate T helper-B cell interaction mediated by microbial superantigen (SAg); (b) contact-dependent interaction with CD40L+, but not CD40L- Jurkat mutant T cell clones; and (c) monoclonal anti-CD40, but not any of a panel of control antibodies. Enhanced B cell Fas\/Apo-1 expression is functionally significant. Coculture of Ramos Burkitt's lymphoma line cells with irradiated SAg-reactive CD4+ T cells with SAg or CD40L+ Jurkat T cells results in B cell apoptosis, evidenced by reduced cell viability and DNA laddering. This process is augmented by the addition of anti-Apo-1\/Fas monoclonal antibody, consistent with an acquired susceptibility to Apo-1\/Fas-mediated apoptosis. These data support an immunoregulatory pathway in which seemingly contradictory signals involving the B cell proliferation\/survival antigen CD40, as well as the Apo-1\/Fas molecule, which mediates programmed cell death of lymphocytes, are linked in the process of human B cell activation.<\/jats:p>","DOI":"10.1084\/jem.182.5.1557","type":"journal-article","created":{"date-parts":[[2004,6,24]],"date-time":"2004-06-24T07:56:10Z","timestamp":1088063770000},"page":"1557-1565","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":187,"title":["CD40 ligation induces Apo-1\/Fas expression on human B lymphocytes and facilitates apoptosis through the Apo-1\/Fas pathway."],"prefix":"10.1084","volume":"182","author":[{"given":"E J","family":"Schattner","sequence":"first","affiliation":[{"name":"Department of Medicine, Hospital for Special Surgery, New York 10021, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K B","family":"Elkon","sequence":"additional","affiliation":[{"name":"Department of Medicine, Hospital for Special Surgery, New York 10021, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D H","family":"Yoo","sequence":"additional","affiliation":[{"name":"Department of Medicine, Hospital for Special Surgery, New York 10021, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Tumang","sequence":"additional","affiliation":[{"name":"Department of Medicine, Hospital for Special Surgery, New York 10021, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P H","family":"Krammer","sequence":"additional","affiliation":[{"name":"Department of Medicine, Hospital for Special Surgery, New York 10021, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M K","family":"Crow","sequence":"additional","affiliation":[{"name":"Department of Medicine, Hospital for Special Surgery, New York 10021, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S M","family":"Friedman","sequence":"additional","affiliation":[{"name":"Department of Medicine, Hospital for Special Surgery, New York 10021, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1995,11,1]]},"container-title":["The Journal of experimental medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/182\/5\/1557\/1678077\/1557.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/182\/5\/1557\/1678077\/1557.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T07:04:08Z","timestamp":1690268648000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/182\/5\/1557\/25734\/CD40-ligation-induces-Apo-1-Fas-expression-on"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,11,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1995,11,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.182.5.1557","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,11,1]]}}}