{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T15:05:54Z","timestamp":1791903954314,"version":"4.3.4"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"6","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1995,12,1]]},"abstract":"<jats:p>Human urinary tract infection is an infectious disease that depends on a series of host-microbial interactions. The bacteria first colonize the colon and then the periurethral\/vaginal areas; they ascend to and infect first the bladder and then the kidneys. Expression of Escherichia coli P-fimbriae constitutes the strongest correlation to renal pathogenicity, but is also related to first-time cystitis in children. The role of P-fimbriae in the preceding steps in the infectious process is unknown. To examine this, we constructed, from a P-fimbriated E. coli strain with a class II G-adhesin preferentially binding to globoside, one isogenic mutant lacking the G-adhesin and another isogenic mutant in which we replaced the papG class II allele with a class III adhesin preferentially binding to the Forssman antigen. We report here the comparison of the adhesin knockout mutant (DS17-8) and the class-switch mutant (DS17-1) with the wild-type (DS17) for in vivo colonization of the gut, vagina, and bladder of cynomolgus monkeys. It was recently shown that the class II tip G-adhesin is a prerequisite for acute pyelonephritis to occur in the monkey model in the absence of other kidney-specific adhesins or obstruction of the urinary flow. Here we show that it is not required for bladder infection but gives a competitive advantage in mixed infections. In the vagina and colon, the G-adhesin gives no competitive advantage.<\/jats:p>","DOI":"10.1084\/jem.182.6.1695","type":"journal-article","created":{"date-parts":[[2004,6,24]],"date-time":"2004-06-24T07:56:10Z","timestamp":1088063770000},"page":"1695-1702","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":31,"title":["The PapG-adhesin at the tip of P-fimbriae provides Escherichia coli with a competitive edge in experimental bladder infections of cynomolgus monkeys."],"prefix":"10.1084","volume":"182","author":[{"given":"J","family":"Winberg","sequence":"first","affiliation":[{"name":"Department of Women and Child Health, Karolinska Hospital, Stockholm, Sweden."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"M\u00f6llby","sequence":"additional","affiliation":[{"name":"Department of Women and Child Health, Karolinska Hospital, Stockholm, Sweden."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Bergstr\u00f6m","sequence":"additional","affiliation":[{"name":"Department of Women and Child Health, Karolinska Hospital, Stockholm, Sweden."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K A","family":"Karlsson","sequence":"additional","affiliation":[{"name":"Department of Women and Child Health, Karolinska Hospital, Stockholm, Sweden."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I","family":"Leonardsson","sequence":"additional","affiliation":[{"name":"Department of Women and Child Health, Karolinska Hospital, Stockholm, Sweden."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M A","family":"Milh","sequence":"additional","affiliation":[{"name":"Department of Women and Child Health, Karolinska Hospital, Stockholm, Sweden."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Teneberg","sequence":"additional","affiliation":[{"name":"Department of Women and Child Health, Karolinska Hospital, Stockholm, Sweden."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Haslam","sequence":"additional","affiliation":[{"name":"Department of Women and Child Health, Karolinska Hospital, Stockholm, Sweden."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B I","family":"Marklund","sequence":"additional","affiliation":[{"name":"Department of Women and Child Health, Karolinska Hospital, Stockholm, Sweden."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Normark","sequence":"additional","affiliation":[{"name":"Department of Women and Child Health, Karolinska Hospital, Stockholm, Sweden."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1995,12,1]]},"container-title":["The Journal of experimental medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/182\/6\/1695\/1678063\/1695.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/182\/6\/1695\/1678063\/1695.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T07:03:54Z","timestamp":1690268634000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/182\/6\/1695\/25475\/The-PapG-adhesin-at-the-tip-of-P-fimbriae-provides"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,12,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1995,12,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.182.6.1695","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,12,1]]}}}