{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:28:26Z","timestamp":1789950506410,"version":"4.0.1"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"1","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1996,1,1]]},"abstract":"<jats:p>CD45 expression is essential for immunoglobulin (Ig)-mediated B cell activation. Treatments with either anti-Ig or anti-CD45 suggest that CD45 may facilitate early signaling events such as calcium mobilization, and phosphoinositide hydrolyis as well as later events leading to transcription of genes such as c-myc. To examine the role of CD45 more extensively, CD45-deficient mice were generated by disruption of exon 6. Although normal numbers of B cells were found in peripheral lymphoid tissues, CD45-deficient cells failed to proliferate upon IgM crosslinking. In the present study, we demonstrate that the fraction of high buoyant density B cells is reduced while low buoyant density cells are increased. Moreover, there is a significant decline in the number of splenic B cells of the mature IgDhi, IgMlo phenotype. Although both the basal and anti-Ig-induced levels of phosphorylation of Ig-alpha and phospholipase C gamma 2 are indistinguishable from that observed in CD45+ control B cells, a major distinction was found in Ca2+ mobilization. While anti-Ig-induced mobilization of intracellular Ca2+ stores was normal, influx from extracellular sources was abrogated. This finding reveals a novel pathway of regulating B cell responses mediated by CD45.<\/jats:p>","DOI":"10.1084\/jem.183.1.329","type":"journal-article","created":{"date-parts":[[2004,6,24]],"date-time":"2004-06-24T07:56:10Z","timestamp":1088063770000},"page":"329-334","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":72,"title":["Immunoglobulin-mediated signal transduction in B cells from CD45-deficient mice."],"prefix":"10.1084","volume":"183","author":[{"given":"T","family":"Benatar","sequence":"first","affiliation":[{"name":"Wellesley Hospital Research Institute, University of Toronto, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Carsetti","sequence":"additional","affiliation":[{"name":"Wellesley Hospital Research Institute, University of Toronto, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Furlonger","sequence":"additional","affiliation":[{"name":"Wellesley Hospital Research Institute, University of Toronto, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N","family":"Kamalia","sequence":"additional","affiliation":[{"name":"Wellesley Hospital Research Institute, University of Toronto, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Mak","sequence":"additional","affiliation":[{"name":"Wellesley Hospital Research Institute, University of Toronto, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C J","family":"Paige","sequence":"additional","affiliation":[{"name":"Wellesley Hospital Research Institute, University of Toronto, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1996,1,1]]},"container-title":["The Journal of experimental medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/183\/1\/329\/1678075\/329.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/183\/1\/329\/1678075\/329.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T07:03:57Z","timestamp":1690268637000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/183\/1\/329\/58334\/Immunoglobulin-mediated-signal-transduction-in-B"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,1,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1996,1,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.183.1.329","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,1,1]]}}}