{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T17:31:06Z","timestamp":1787160666987,"version":"build-2736575974"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"4","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1996,4,1]]},"abstract":"<jats:p>The cellular basis of T cell memory is a controversial issue and progress has been hampered by the inability to induce and to trace long-term memory T cells specific for a defined antigen in vivo. By using the murine model of lymphocytic choriomeningitis virus (LCMV) infection and an adoptive transfer system with CD8+ T cells from transgenic mice expressing an LCMV-specific T cell receptor, a population of authentic memory T cells specific for LCMV was generated and analyzed in vivo. The transgenic T cells that have expanded (1,000-fold) and then decreased (10-fold) in LCMV-infected C57BL\/6 recipient mice exhibited the following characteristics: they were (a) of larger average cell size than their naive counterparts but smaller than day 8 effector cells; (b) heterogeneous with respect to expression of cell surface \"memory\" markers; and (c) directly cytolytic when isolated from recipient spleens. The time-dependent proliferative activity of these LCMV-specific memory T cells was analyzed in the recipients by bromodeoxyuridine labeling experiments in vivo. The experiments revealed that LCMV-specific CD8+ memory T cells can persist in LCMV-immune mice for extended periods of time (&amp;gt;2 mo) in the absence of cell division; the memory population as a whole survived beyond 11 mo.<\/jats:p>","DOI":"10.1084\/jem.183.4.1367","type":"journal-article","created":{"date-parts":[[2004,6,24]],"date-time":"2004-06-24T03:56:10Z","timestamp":1088049370000},"page":"1367-1375","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":165,"title":["Visualization, characterization, and turnover of CD8+ memory T cells in virus-infected hosts."],"prefix":"10.1084","volume":"183","author":[{"given":"C","family":"Zimmerman","sequence":"first","affiliation":[{"name":"Institute for Medical Microbiology and Hygiene, Department of Immunology, University of Freiburg, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Brduscha-Riem","sequence":"additional","affiliation":[{"name":"Institute for Medical Microbiology and Hygiene, Department of Immunology, University of Freiburg, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Blaser","sequence":"additional","affiliation":[{"name":"Institute for Medical Microbiology and Hygiene, Department of Immunology, University of Freiburg, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R M","family":"Zinkernagel","sequence":"additional","affiliation":[{"name":"Institute for Medical Microbiology and Hygiene, Department of Immunology, University of Freiburg, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Pircher","sequence":"additional","affiliation":[{"name":"Institute for Medical Microbiology and Hygiene, Department of Immunology, University of Freiburg, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1996,4,1]]},"container-title":["The Journal of experimental medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/183\/4\/1367\/1678430\/1367.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/183\/4\/1367\/1678430\/1367.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T03:12:35Z","timestamp":1690254755000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/183\/4\/1367\/58400\/Visualization-characterization-and-turnover-of-CD8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,4,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1996,4,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.183.4.1367","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,4,1]]}}}