{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T18:41:59Z","timestamp":1791052919848,"version":"4.1.0"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"5","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1996,5,1]]},"abstract":"<jats:p>CD40-CD40 ligand (CD40L) interaction is required for the generation of antibody responses to T-dependent antigens as well as for the development of germinal centers and memory B cells. The role of the CD40-CD40L interaction in the induction of antigen-specific. Th cells and in mediating Th cell effector functions other than cognate help for B cells is less well understood. Using CD40- and CD40L-deficient mice together with lymphocytic choriomeningitis virus and vesicular stomatitis virus as viral model antigens, this study corroborates earlier findings that no lg isotype switching of virus-specific antibodies was measurable upon infection of CD40- or CD40L-deficient mice. In contrast, in vivo induction of virus-specific CD4+ T cells measured by proliferation and cytokine secretion of primed virus-specific Th cells in vitro was not crucially dependent on the CD40-CD40L interaction. In addition, virus-specific Th cells primed in a CD40-deficient environment, adoptively transferred into CD40-competent recipients, were able to mediate lg isotype switch. Th-mediated effector functions distinct from and in addition to T-B collaboration were analyzed in CD40- and CD40L-deficient and normal mice: (a) local inflammatory reactions upon LCMV infection mediated by LCMV-specific Th cells were not dependent on a functional CD40-CD40L interaction, (b) cytokine-mediated protection by CD4+ T cells primed by vesicular stomatitis virus against a challenge infection with recombinant vaccinia virus expressing the glycoprotein of vesicular stomatitis virus was found to be equivalent in CD40L-deficient and normal mice. Thus, CD40-CD40L interaction plays a crucial role in T-B interactions for Th-dependent activation of B cells but not, or to a much lesser extent, in T cell activation, antigen-specific Th cell responses in vitro, and for interleukin-mediated Th cell effector functions in vivo.<\/jats:p>","DOI":"10.1084\/jem.183.5.2209","type":"journal-article","created":{"date-parts":[[2004,6,24]],"date-time":"2004-06-24T03:56:10Z","timestamp":1088049370000},"page":"2209-2218","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":104,"title":["CD40-CD40 ligand interactions are critical in T-B cooperation but not for other anti-viral CD4+ T cell functions."],"prefix":"10.1084","volume":"183","author":[{"given":"A","family":"Oxenius","sequence":"first","affiliation":[{"name":"Department of Pathology, University of Z\u00fcrich, Switzerland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K A","family":"Campbell","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Z\u00fcrich, Switzerland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C R","family":"Maliszewski","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Z\u00fcrich, Switzerland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Kishimoto","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Z\u00fcrich, Switzerland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Kikutani","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Z\u00fcrich, Switzerland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Hengartner","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Z\u00fcrich, Switzerland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R M","family":"Zinkernagel","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Z\u00fcrich, Switzerland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M F","family":"Bachmann","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Z\u00fcrich, Switzerland."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1996,5,1]]},"container-title":["The Journal of experimental medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/183\/5\/2209\/1678762\/2209.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/183\/5\/2209\/1678762\/2209.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T03:16:21Z","timestamp":1690254981000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/183\/5\/2209\/57667\/CD40-CD40-ligand-interactions-are-critical-in-T-B"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1996,5,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.183.5.2209","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,5,1]]}}}