{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T08:48:19Z","timestamp":1791103699042,"version":"4.1.0"},"reference-count":0,"publisher":"Rockefeller University Press","issue":"5","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1996,5,1]]},"abstract":"<jats:p>We studied the effects of various chemokines including neutrophil-activating peptide 2 (NAP-2), beta-thromboglobulin (beta-TG), platelet factor 4 (PF-4), melanoma growth stimulating activity (GRO), gamma interferon-induced protein (IP-10), regulated on activation, normal T expressed and secreted (RANTES), macrophage inflammatory protein 1 alpha (MIP-1 alpha), MIP-1 beta, and monocyte chemotactic protein 1 (MCP-1) on Immunoglobulin (IgE) and IgG4 production by human B cells. None of these chemokines with or without interleukin (IL-4), anti-CD40 or -CD58 monoclonal antibody (mAb), induced IgE and IgG4 production by B cells from nonatopic donors. However, RANTES and MIP-1 alpha selectively enhanced IgE and IgG4 production induced by IL-4 plus anti-CD40 or -CD58 mAb without affecting production of IgM, IgG1, IgG2, IgG3, IgA1, or IgA2, whereas other chemokines failed to do so. Enhancement of IgE and IgG4 production by RANTES and MIP-1 alpha was specifically blocked by anti-RANTES mAb and anti-MIP-1 alpha antibody (Ab), respectively, whereas anti-IL-5 mAb, anti-IL-6 mAb, anti-IL-10 Ab, anti-IL-13 Ab, and anti-tumor necrosis factor-alpha mAb failed to do so. Purified surface IgE positive (slgE4) and slgG4+ B cells generated either in vitro or in vivo spontaneously produced IgE and IgG4, respectively, whereas sIgE- and sIgG4- B cells failed to do so. RANTES and MIP-1 alpha enhanced spontaneous IgE and IgG4 production in slgE+ and slgG4- B cells, respectively, whereas neither RANTES nor MIP-1 alpha did so in sIgE- or sIgG4- B cells. Purified sIgE4+ and sIgG4+, but not sIgE- or sIgG4- B cells, generated in vitro and in vivo expressed receptors for RANTES and MIP-1 alpha, whereas they failed to express receptors for other chemokines. These findings indicate that RANTES and MIP-1 alpha enhance IgE and IgG4 production by directly stimulating sIgE+ and sIgG4+ B cells.<\/jats:p>","DOI":"10.1084\/jem.183.5.2397","type":"journal-article","created":{"date-parts":[[2004,6,24]],"date-time":"2004-06-24T07:56:10Z","timestamp":1088063770000},"page":"2397-2402","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":73,"title":["RANTES and macrophage inflammatory protein 1 alpha selectively enhance immunoglobulin (IgE) and IgG4 production by human B cells."],"prefix":"10.1084","volume":"183","author":[{"given":"H","family":"Kimata","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Yunichika Central Hospital, Kyoto, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Yunichika Central Hospital, Kyoto, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Ishioka","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Yunichika Central Hospital, Kyoto, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Fujimoto","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Yunichika Central Hospital, Kyoto, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I","family":"Lindley","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Yunichika Central Hospital, Kyoto, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Furusho","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Yunichika Central Hospital, Kyoto, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1996,5,1]]},"container-title":["The Journal of experimental medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/183\/5\/2397\/1678724\/2397.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/183\/5\/2397\/1678724\/2397.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T07:15:36Z","timestamp":1690269336000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/183\/5\/2397\/57687\/RANTES-and-macrophage-inflammatory-protein-1-alpha"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1996,5,1]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.183.5.2397","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,5,1]]}}}