{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:02:49Z","timestamp":1792789369559,"version":"4.5.0"},"reference-count":27,"publisher":"Rockefeller University Press","issue":"6","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1996,12,1]]},"abstract":"<jats:p>To study the role of CD8\u03b2 in T cell function, we derived a CD8\u03b1\/\u03b2\u2212 (CD8\u2212\/\u2212) T cell hybridoma of the H-2Kd\u2013restricted N9 cytotoxic T lymphocyte clone specific for a photoreactive derivative of the Plasmodium berghei circumsporozoite peptide PbCS 252-260. This hybridoma was transfected either with CD8\u03b1 alone or together with CD8\u03b2. All three hybridomas released interleukin 2 upon incubation with L cells expressing Kd\u2013peptide derivative complexes, though CD8\u03b1\/\u03b2 cells did so more efficiently than CD8\u03b1\/\u03b1 and especially CD8\u2212\/\u2212 cells. More strikingly, only CD8\u03b1\/\u03b2 cells were able to recognize a weak agonist peptide derivative variant. This recognition was abolished by Fab\u2032 fragments of the anti-Kd \u03b13 monoclonal antibody SF11.1.1 or substitution of Kd D-227 with K, both conditions known to impair CD8 coreceptor function. T cell receptor (TCR) photoaffinity labeling indicated that TCR\u2013ligand binding on CD8\u03b1\/\u03b2 cells was \u223c5- and 20-fold more avid than on CD8\u03b1\/a and CD8\u2212\/\u2212 cells, respectively. SF1-1.1.1 Fab\u2032 or Kd mutation D227K reduced the TCR photoaffinity labeling on CD8\u03b1\/\u03b2 cells to approximately the same low levels observed on CD8\u2212\/\u2212 cells. These results indicate that CD8\u03b1\/\u03b2 is a more efficient coreceptor than CD8\u03b1\/\u03b1, because it more avidly strengthens TCR\u2013ligand binding.<\/jats:p>","DOI":"10.1084\/jem.184.6.2439","type":"journal-article","created":{"date-parts":[[2004,6,24]],"date-time":"2004-06-24T07:56:10Z","timestamp":1088063770000},"page":"2439-2444","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":65,"title":["CD8\u03b2 Increases CD8 Coreceptor Function and Participation in TCR\u2013Ligand Binding"],"prefix":"10.1084","volume":"184","author":[{"given":"Valery","family":"Renard","sequence":"first","affiliation":[{"name":"From the *Centre d'Immunologie Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Centre National de la Recherche Scientifique de Marseille-Luminy, Case 906, 13288 Marseille, Cedex 09, France; and \u2021Ludwig Institute for Cancer Research, Lausanne Branch, University of Lausanne, 1066 Epalinges, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro","family":"Romero","sequence":"additional","affiliation":[{"name":"From the *Centre d'Immunologie Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Centre National de la Recherche Scientifique de Marseille-Luminy, Case 906, 13288 Marseille, Cedex 09, France; and \u2021Ludwig Institute for Cancer Research, Lausanne Branch, University of Lausanne, 1066 Epalinges, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Vivier","sequence":"additional","affiliation":[{"name":"From the *Centre d'Immunologie Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Centre National de la Recherche Scientifique de Marseille-Luminy, Case 906, 13288 Marseille, Cedex 09, France; and \u2021Ludwig Institute for Cancer Research, Lausanne Branch, University of Lausanne, 1066 Epalinges, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernard","family":"Malissen","sequence":"additional","affiliation":[{"name":"From the *Centre d'Immunologie Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Centre National de la Recherche Scientifique de Marseille-Luminy, Case 906, 13288 Marseille, Cedex 09, France; and \u2021Ludwig Institute for Cancer Research, Lausanne Branch, University of Lausanne, 1066 Epalinges, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Immanuel F.","family":"Luescher","sequence":"additional","affiliation":[{"name":"From the *Centre d'Immunologie Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Centre National de la Recherche Scientifique de Marseille-Luminy, Case 906, 13288 Marseille, Cedex 09, France; and \u2021Ludwig Institute for Cancer Research, Lausanne Branch, University of Lausanne, 1066 Epalinges, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1996,12,1]]},"reference":[{"key":"2023072819492070500_B1","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1146\/annurev.iy.10.040192.003241","article-title":"The T cell receptor as a multicomponent signaling machine: CD4\/CD8 coreceptors and CD45 in T cell activation","volume":"10","author":"Janeway","year":"1992","journal-title":"Annu Rev Immunol"},{"key":"2023072819492070500_B2","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1096\/fasebj.9.1.7821755","article-title":"A structural view of CD4 and CD8","volume":"9","author":"Leahy","year":"1995","journal-title":"FASEB J"},{"key":"2023072819492070500_B3","doi-asserted-by":"crossref","first-page":"3646","DOI":"10.4049\/jimmunol.140.10.3646","article-title":"Molecular linkage of the Ly-3 and Ly-2 genes. 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