{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T11:43:50Z","timestamp":1786794230841,"version":"build-2736575974"},"reference-count":26,"publisher":"Rockefeller University Press","issue":"10","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1997,5,19]]},"abstract":"<jats:p>The emergence of multidrug-resistant organisms and the failure to eradicate infection by a number of important pathogens has led to increased efforts to develop vaccines to prevent infectious diseases. However, the nature of the immune response to vaccination with a given antigen can be complex and unpredictable. An example is the galactose\u2013 and N-acetylgalactosamine\u2013inhibitable lectin, a surface antigen of Entamoeba histolytica that has been identified as a major candidate in a vaccine to prevent amebiasis. Vaccination with the lectin can induce protective immunity to amebic liver abscess in some animals, but others of the same species exhibit exacerbations of disease after vaccination. To better understand this phenomenon, we used recombinant proteins corresponding to four distinct domains of the molecule, and synthetic peptides to localize both protective and exacerbative epitopes of the heavy chain subunit of the lectin. We show that protective immunity after vaccination can be correlated with the development of an antibody response to a region of 25 amino acid residues of the lectin, and have confirmed the importance of the antibody response to this region by passive immunization studies. In addition, we show that exacerbation of disease can be linked to the development of antibodies that bind to an NH2-terminal domain of the lectin. These findings are clinically relevant, as individuals who are colonized with E. histolytica but are resistant to invasive disease have a high prevalence of antibodies to the protective epitope(s), compared to individuals with a history of invasive amebiasis. These studies should enable us to develop an improved vaccine for amebiasis, and provide a model for the identification of protective and exacerbative epitopes of complex antigens.<\/jats:p>","DOI":"10.1084\/jem.185.10.1793","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:49:30Z","timestamp":1027702170000},"page":"1793-1801","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":68,"title":["Identification of an Epitope on the <i>Entamoeba histolytica<\/i> 170-kD Lectin Conferring Antibody-mediated Protection against Invasive Amebiasis"],"prefix":"10.1084","volume":"185","author":[{"given":"Hannelore","family":"Lotter","sequence":"first","affiliation":[{"name":"From the *Department of Molecular Biology, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany; and the \u2021Department of Medicine and Molecular Microbiology, Washington University, School of Medicine, St. Louis, Missouri 63110"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tonghai","family":"Zhang","sequence":"additional","affiliation":[{"name":"From the *Department of Molecular Biology, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany; and the \u2021Department of Medicine and Molecular Microbiology, Washington University, School of Medicine, St. Louis, Missouri 63110"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karl B.","family":"Seydel","sequence":"additional","affiliation":[{"name":"From the *Department of Molecular Biology, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany; and the \u2021Department of Medicine and Molecular Microbiology, Washington University, School of Medicine, St. Louis, Missouri 63110"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Samuel L.","family":"Stanley","sequence":"additional","affiliation":[{"name":"From the *Department of Molecular Biology, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany; and the \u2021Department of Medicine and Molecular Microbiology, Washington University, School of Medicine, St. Louis, Missouri 63110"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Egbert","family":"Tannich","sequence":"additional","affiliation":[{"name":"From the *Department of Molecular Biology, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany; and the \u2021Department of Medicine and Molecular Microbiology, Washington University, School of Medicine, St. Louis, Missouri 63110"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1997,5,19]]},"reference":[{"key":"2023072507000790500_B1","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1093\/clinids\/8.2.228","article-title":"Problems in recognition and diagnosis of amebiasis: estimation of the global magnitude of morbidity and 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I. Mitogenicity of Entamoeba histolyticaextracts for human peripheral T lymphocytes","volume":"126","author":"Diamantstein","year":"1981","journal-title":"J Immunol"},{"key":"2023072507000790500_B22","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1093\/infdis\/151.5.816","article-title":"N-acetyl-d-galactosamine\u2013 inhibitable adherence lectin of Entamoeba histolytica.II. Mitogenic activity for human lymphocytes","volume":"151","author":"Salata","year":"1985","journal-title":"J Infect Dis"},{"key":"2023072507000790500_B23","doi-asserted-by":"crossref","first-page":"1772","DOI":"10.1128\/iai.61.5.1772-1778.1993","article-title":"Neutralizing monoclonal antibody epitopes of the Entamoeba histolyticagalactose adhesin map to the cysteine-rich extracellular domain on the 170-kilodalton subunit","volume":"61","author":"Mann","year":"1993","journal-title":"Infect Immun"},{"key":"2023072507000790500_B24","doi-asserted-by":"crossref","first-page":"2605","DOI":"10.1128\/iai.62.6.2605-2608.1994","article-title":"Protection of gerbils from amebic liver abscess by immunization with a recombinant protein derived from the 170 kDa surface adhesin of Entamoeba histolytica.","volume":"62","author":"Zhang","year":"1994","journal-title":"Infect Immun"},{"key":"2023072507000790500_B25","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1093\/infdis\/171.3.645","article-title":"A recombinant cysteine-rich section of the Entamoeba histolyticagalactose-inhibitable lectin is efficacious as a subunit vaccine in the gerbil model of amebic liver abscess","volume":"171","author":"Soong","year":"1995","journal-title":"J Infect Dis"},{"key":"2023072507000790500_B26"}],"container-title":["The Journal of Experimental Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/185\/10\/1793\/1679572\/5535.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/185\/10\/1793\/1679572\/5535.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T07:29:02Z","timestamp":1690270142000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/185\/10\/1793\/25148\/Identification-of-an-Epitope-on-the-Entamoeba"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,5,19]]},"references-count":26,"journal-issue":{"issue":"10","published-print":{"date-parts":[[1997,5,19]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.185.10.1793","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,5,19]]}}}