{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T10:49:58Z","timestamp":1770720598119,"version":"3.49.0"},"reference-count":24,"publisher":"Rockefeller University Press","issue":"11","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1997,6,2]]},"abstract":"<jats:p>The N-propionylated group B meningococcal polysaccharide (NPrGBMP) mimics a unique protective epitope on the surface of group B meningococci (GBM) and Escherichia coli K1. Using a series of monoclonal antibodies (mAbs) induced by the NPrGBMP\u2013monomeric tetanus toxoid (TT) conjugate vaccine it was demonstrated that mAbs having specificities for both extended and conventional short segments of the NPrGBMP were formed, but only the former were bactericidal, and\/or gave passive protection against live challenge by GBM. The failure of mAbs specific for short epitopes to protect was further established when (NeuPr)4\u2013TT was used as the vaccine. Of all the mAbs produced that were specific for short internal segments of the NPrGBMP, none were protective, despite the fact that most of them cross-react with the GBM capsular polysaccharide. In contrast, most of the protective mAbs produced by NPrGBMP\u2013 TT did not recognize the group B meningococcal polysaccharide (GBMP) unless it was present in its aggregated high molecular weight form. The bactericidal epitope mimicked by the NPrGBMP was shown to be ubiquitous in the capsule of both GBM and E. coli K1 using immunogold labeling techniques and, because of its unique properties, its identification could be significant in the development of a comprehensive conjugate vaccine against group B meningococcal meningitis. This is because most known human \u03b1(2\u20138)-polysialic acid self-antigens can be accommodated in 30\u201350 \u03b1(2\u20138)-linked sialic acid residues, which is roughly equivalent to an 11-kD length of the GBMP. It has been hypothesized that the formation of the protective epitope on the surface of GBM is due to the interaction of helical segments of the GBMP with another molecule and that the protective epitope is mimicked by the NPrGBMP. Support for the above hypothesis is provided by the fact that the protective NPrGBMP epitope has a similar unusual length dependency to that of the GBMP epitope.<\/jats:p>","DOI":"10.1084\/jem.185.11.1929","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:49:30Z","timestamp":1027702170000},"page":"1929-1938","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":79,"title":["<i>N<\/i>-Propionylated Group B Meningococcal Polysaccharide Mimics a Unique Bactericidal Capsular Epitope in Group B <i>Neisseria meningitidis<\/i>"],"prefix":"10.1084","volume":"185","author":[{"given":"Robert A.","family":"Pon","sequence":"first","affiliation":[{"name":"From the Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario, Canada K1A 0R6"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michele","family":"Lussier","sequence":"additional","affiliation":[{"name":"From the Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario, Canada K1A 0R6"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing-Ling","family":"Yang","sequence":"additional","affiliation":[{"name":"From the Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario, Canada K1A 0R6"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harold J.","family":"Jennings","sequence":"additional","affiliation":[{"name":"From the Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario, Canada K1A 0R6"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1997,6,2]]},"reference":[{"key":"2023072507000445900_B1","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1093\/infdis\/126.5.514","article-title":"Immunological response of man to group B meningococcal polysaccharide antigens","volume":"126","author":"Wyle","year":"1972","journal-title":"J Infect Dis"},{"key":"2023072507000445900_B2","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.4049\/jimmunol.127.3.1011","article-title":"Immunochemistry of groups A, B, and C meningococcal polysaccharide\u2013tetanus toxoid conjugates","volume":"127","author":"Jennings","year":"1981","journal-title":"J Immunol"},{"key":"2023072507000445900_B3","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1016\/0264-410X(94)00007-A","article-title":"Immunogenicity of meningococcal B polysaccharide conjugated to tetanus toxoid or CRM197 via adipic acid dihydrazide","volume":"13","author":"Bartolini","year":"1995","journal-title":"Vaccine"},{"key":"2023072507000445900_B4","doi-asserted-by":"crossref","first-page":"1926","DOI":"10.1016\/S0021-9258(19)41784-5","article-title":"Structural determination of the sialic acid polysaccharide of Neisseria meningitisserogroups B and C with carbon 13 nuclear magnetic resonance","volume":"250","author":"Bhattacharjee","year":"1975","journal-title":"J Biol Chem"},{"key":"2023072507000445900_B5","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1016\/0006-291X(83)91490-0","article-title":"Occurrence of \u03b1-2\u20138 linked polysialosyl units in a neural cell adhesion molecule","volume":"112","author":"Finne","year":"1983","journal-title":"Biochem Biophys Res Commun"},{"key":"2023072507000445900_B6","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1093\/glycob\/2.1.5","article-title":"Polysialylation: from bacteria to brains","volume":"2","author":"Troy","year":"1992","journal-title":"Glycobiology"},{"key":"2023072507000445900_B7","doi-asserted-by":"crossref","first-page":"12224","DOI":"10.1016\/S0021-9258(19)86453-0","article-title":"Isolation and characterization of two isomers of brain tetrasialogangliosides","volume":"254","author":"Ando","year":"1979","journal-title":"J Biol Chem"},{"key":"2023072507000445900_B8","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/S1201-9712(97)90080-1","article-title":"N-Propionylated group B meningococcal polysaccharide glycoconjugate vaccine against group B meningococcal meningitis","volume":"1","author":"Jennings","year":"1996","journal-title":"Int J Infect Dis"},{"key":"2023072507000445900_B9","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.1073\/pnas.82.4.1194","article-title":"NZB mouse system for production of monoclonal antibodies to weak bacterial antigens: isolation of an IgG antibody to the polysaccharide capsules of Escherichia coliK1 and group B meningococci","volume":"82","author":"Frosch","year":"1985","journal-title":"Proc Natl Acad Sci USA"},{"key":"2023072507000445900_B10","doi-asserted-by":"crossref","first-page":"2651","DOI":"10.4049\/jimmunol.134.4.2651","article-title":"Determinant specificities of the groups B and C polysaccharides of Neisseria meningitidis.","volume":"134","author":"Jennings","year":"1985","journal-title":"J Immunol"},{"key":"2023072507000445900_B11","doi-asserted-by":"crossref","first-page":"1481","DOI":"10.1093\/infdis\/171.6.1481","article-title":"Antibodies to polysialic acid and its N-propyl derivative: binding properties and interaction with human embryonal brain glycopeptides","volume":"171","author":"H\u00e4yrinen","year":"1995","journal-title":"J Infect Dis"},{"key":"2023072507000445900_B12","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1084\/jem.168.2.699","article-title":"The epitope association with the binding of the capsular polysaccharide of the group B meningococcus and of Escherichia coliK1 to a human monoclonal macroglobulin, IgM NOV","volume":"168","author":"Kabat","year":"1988","journal-title":"J Exp Med"},{"key":"2023072507000445900_B13","doi-asserted-by":"crossref","first-page":"4996","DOI":"10.1021\/bi00136a012","article-title":"Helical epitope of the group B meningococcal \u03b1(2\u20138)-linked sialic acid polysaccharide","volume":"31","author":"Brisson","year":"1992","journal-title":"Biochemistry"},{"key":"2023072507000445900_B14","doi-asserted-by":"crossref","first-page":"4007","DOI":"10.1021\/bi00066a022","article-title":"Comparison of the conformation of the epitope of \u03b1(2\u20138)-polysialic acid with its reduced and N-acetyl derivatives","volume":"32","author":"Baumann","year":"1993","journal-title":"Biochemistry"},{"key":"2023072507000445900_B15","doi-asserted-by":"crossref","first-page":"6737","DOI":"10.1021\/bi00020a019","article-title":"Evidence for the extended helical nature of polysaccharide epitopes. The 2.8 \u212b resolution structure and thermodynamics of ligand binding of an antigen binding fragment specific of \u03b1(2\u20138)-polysialic acid","volume":"34","author":"Evans","year":"1995","journal-title":"Biochemistry"},{"key":"2023072507000445900_B16","doi-asserted-by":"crossref","first-page":"1708","DOI":"10.4049\/jimmunol.137.5.1708","article-title":"Induction of meningococcal group B polysaccharide-specific IgG antibodies in mice by using an N-propionylated B polysaccharide\u2013 tetanus toxoid conjugate vaccine","volume":"137","author":"Jennings","year":"1986","journal-title":"J Immunol"},{"key":"2023072507000445900_B17","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1016\/0882-4010(89)90087-9","article-title":"Protective efficacy of mouse serum to the N-propionyl derivative of meningococcal group B polysaccharide","volume":"6","author":"Ashton","year":"1989","journal-title":"Microbiol Pathog"},{"key":"2023072507000445900_B18","doi-asserted-by":"crossref","first-page":"1207","DOI":"10.1084\/jem.165.4.1207","article-title":"N-propionylated group B meningococcal polysaccharide mimics a unique epitope on group B Neisseria meningitidis.","volume":"165","author":"Jennings","year":"1987","journal-title":"J Exp Med"},{"key":"2023072507000445900_B19","doi-asserted-by":"crossref","first-page":"3585","DOI":"10.4049\/jimmunol.142.10.3585","article-title":"Unique intermolecular bactericidal epitope involving the homosialopolysaccharide capsule on the cell surface of group B Neisseria meningitidis and Escherichia coliK1","volume":"142","author":"Jennings","year":"1989","journal-title":"J Immunol"},{"key":"2023072507000445900_B20","doi-asserted-by":"crossref","first-page":"4797","DOI":"10.1016\/S0021-9258(19)42390-9","article-title":"Studies on the group-specific polysaccharide of Neisseria meningitidisserogroup X and an improved procedure for its isolation","volume":"249","author":"Bundle","year":"1974","journal-title":"J Biol Chem"},{"key":"2023072507000445900_B21","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1016\/0006-3002(57)90254-8","article-title":"Quantitative estimation of sialic acids. II. A colorimetric resorcinol\u2013hydrochloric acid method","volume":"24","author":"Svennerholm","year":"1957","journal-title":"Biochim Biophys Acta"},{"key":"2023072507000445900_B22"},{"key":"2023072507000445900_B23","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1093\/infdis\/175.2.364","article-title":"Preclinical evaluation of a novel group B meningococcal conjugate vaccine that elicits bactericidal activity in both mice and non-human primates","volume":"175","author":"Tai","year":"1997","journal-title":"J Infect Dis"},{"key":"2023072507000445900_B24","doi-asserted-by":"crossref","first-page":"8915","DOI":"10.1016\/S0021-9258(19)52486-3","article-title":"Lipid on capsular polysaccharides of gram negative bacteria","volume":"256","author":"Gotschlich","year":"1981","journal-title":"J Biol Chem"}],"container-title":["The Journal of Experimental Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/185\/11\/1929\/1679558\/96-2133.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/185\/11\/1929\/1679558\/96-2133.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T07:29:00Z","timestamp":1690270140000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/185\/11\/1929\/6992\/N-Propionylated-Group-B-Meningococcal"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,6,2]]},"references-count":24,"journal-issue":{"issue":"11","published-print":{"date-parts":[[1997,6,2]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.185.11.1929","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,6,2]]}}}