{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T10:18:49Z","timestamp":1792664329124,"version":"4.5.0"},"reference-count":45,"publisher":"Rockefeller University Press","issue":"4","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1997,2,17]]},"abstract":"<jats:p>The binding of immunoglobulin E (IgE) to high affinity IgE receptors (Fc\u03b5RI) expressed on the surface of mast cells primes these cells to secrete, upon subsequent exposure to specific antigen, a panel of proinflammatory mediators, which includes cytokines that can also have immunoregulatory activities. This IgE- and antigen-specific mast cell activation and mediator production is thought to be critical to the pathogenesis of allergic disorders, such as anaphylaxis and asthma, and also contributes to host defense against parasites. We now report that exposure to IgE results in a striking (up to 32-fold) upregulation of surface expression of Fc\u03b5RI on mouse mast cells in vitro or in vivo. Moreover, baseline levels of Fc\u03b5RI expression on peritoneal mast cells from genetically IgE-deficient (IgE \u2212\/\u2212) mice are dramatically reduced (by \u223c83%) compared with those on cells from the corresponding normal mice. In vitro studies indicate that the IgE-dependent upregulation of mouse mast cell Fc\u03b5RI expression has two components: an early cycloheximide-insensitive phase, followed by a later and more sustained component that is highly sensitive to inhibition by cycloheximide. In turn, IgE-dependent upregulation of Fc\u03b5RI expression significantly enhances the ability of mouse mast cells to release serotonin, interleukin-6 (IL-6), and IL-4 in response to challenge with IgE and specific antigen. The demonstration that IgE-dependent enhancement of mast cell Fc\u03b5RI expression permits mast cells to respond to antigen challenge with increased production of proinflammatory and immunoregulatory mediators provides new insights into both the pathogenesis of allergic diseases and the regulation of protective host responses to parasites.<\/jats:p>","DOI":"10.1084\/jem.185.4.663","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:49:30Z","timestamp":1027702170000},"page":"663-672","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":333,"title":["IgE Enhances Mouse Mast Cell Fc\u03b5RI Expression In Vitro and In Vivo: Evidence for a Novel Amplification Mechanism in IgE-dependent Reactions"],"prefix":"10.1084","volume":"185","author":[{"given":"Masao","family":"Yamaguchi","sequence":"first","affiliation":[{"name":"From the *Departments of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215; \u2021Division of Immunology, Children's Hospital and Harvard Medical School, Boston, Massachusetts 02215; and the \u00a7Departments of Pediatrics and Medicine, Uniformed Services University of the Health Sciences, F. Edward H\u00e9bert School of Medicine, Bethesda, Maryland 20814"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris S.","family":"Lantz","sequence":"additional","affiliation":[{"name":"From the *Departments of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215; \u2021Division of Immunology, Children's Hospital and Harvard Medical School, Boston, Massachusetts 02215; and the \u00a7Departments of Pediatrics and Medicine, Uniformed Services University of the Health Sciences, F. Edward H\u00e9bert School of Medicine, Bethesda, Maryland 20814"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans C.","family":"Oettgen","sequence":"additional","affiliation":[{"name":"From the *Departments of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215; \u2021Division of Immunology, Children's Hospital and Harvard Medical School, Boston, Massachusetts 02215; and the \u00a7Departments of Pediatrics and Medicine, Uniformed Services University of the Health Sciences, F. Edward H\u00e9bert School of Medicine, Bethesda, Maryland 20814"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ildy M.","family":"Katona","sequence":"additional","affiliation":[{"name":"From the *Departments of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215; \u2021Division of Immunology, Children's Hospital and Harvard Medical School, Boston, Massachusetts 02215; and the \u00a7Departments of Pediatrics and Medicine, Uniformed Services University of the Health Sciences, F. Edward H\u00e9bert School of Medicine, Bethesda, Maryland 20814"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tony","family":"Fleming","sequence":"additional","affiliation":[{"name":"From the *Departments of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215; \u2021Division of Immunology, Children's Hospital and Harvard Medical School, Boston, Massachusetts 02215; and the \u00a7Departments of Pediatrics and Medicine, Uniformed Services University of the Health Sciences, F. Edward H\u00e9bert School of Medicine, Bethesda, Maryland 20814"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ichiro","family":"Miyajima","sequence":"additional","affiliation":[{"name":"From the *Departments of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215; \u2021Division of Immunology, Children's Hospital and Harvard Medical School, Boston, Massachusetts 02215; and the \u00a7Departments of Pediatrics and Medicine, Uniformed Services University of the Health Sciences, F. Edward H\u00e9bert School of Medicine, Bethesda, Maryland 20814"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Pierre","family":"Kinet","sequence":"additional","affiliation":[{"name":"From the *Departments of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215; \u2021Division of Immunology, Children's Hospital and Harvard Medical School, Boston, Massachusetts 02215; and the \u00a7Departments of Pediatrics and Medicine, Uniformed Services University of the Health Sciences, F. Edward H\u00e9bert School of Medicine, Bethesda, Maryland 20814"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen J.","family":"Galli","sequence":"additional","affiliation":[{"name":"From the *Departments of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215; \u2021Division of Immunology, Children's Hospital and Harvard Medical School, Boston, Massachusetts 02215; and the \u00a7Departments of Pediatrics and Medicine, Uniformed Services University of the Health Sciences, F. 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