{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T22:53:23Z","timestamp":1790031203461,"version":"4.0.1"},"reference-count":25,"publisher":"Rockefeller University Press","issue":"7","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1997,4,7]]},"abstract":"<jats:p>High frequencies of cytotoxic T lymphocyte precursors (CTLp) recognizing HIV-1 laboratory strain gene products have been detected in adults within weeks of primary infection. In contrast, HIV-1\u2013specific CTLp are uncommonly detected in infants younger than 6 mo. To address the hypothesis that the use of target cells expressing laboratory strain env gene products might limit the detection of HIV-1 env-specific CTLp in early infancy, recombinant vaccinia vectors (vv) expressing HIV-1 env genes from early isolates of four vertically infected infants were generated. The frequencies of CTLp recognizing target cells infected with vv-expressing env gene products from early isolates and HIV-1 IIIB were serially measured using limiting dilution followed by in vitro stimulation with mAb to CD3. In one infant, the detection of early isolate env-specific CTLp preceded the detection of IIIB-specific CTLp. CTLp recognizing HIV-1 IIIB and infant isolate env were detected by 6 mo of age in two infants. In a fourth infant, HIV-1 IIIB env and early isolate env-specific CTLp were simultaneously detected at 12 mo of age. These results provide evidence that young infants can generate HIV-1\u2013specific CTL responses and provide support for the concept of neonatal vaccination to prevent HIV-1 transmission. However, the early predominance of type-specific CTL detected in some young infants suggests that the use of vaccines based on laboratory strains of HIV-1 may not protect against vertical infection.<\/jats:p>","DOI":"10.1084\/jem.185.7.1153","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:49:30Z","timestamp":1027702170000},"page":"1153-1162","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":52,"title":["Early HIV-1 Envelope-specific Cytotoxic T Lymphocyte Responses in Vertically Infected Infants"],"prefix":"10.1084","volume":"185","author":[{"given":"Cheryl A.","family":"Pikora","sequence":"first","affiliation":[{"name":"From the *Department of Pediatrics, Program in Molecular Medicine, University of Massachusetts Medical Center, Worcester, Massachusetts 01605; and \u2021Therion Biologics Corporation, Cambridge, Massachusetts 02142"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John L.","family":"Sullivan","sequence":"additional","affiliation":[{"name":"From the *Department of Pediatrics, Program in Molecular Medicine, University of Massachusetts Medical Center, Worcester, Massachusetts 01605; and \u2021Therion Biologics Corporation, Cambridge, Massachusetts 02142"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dennis","family":"Panicali","sequence":"additional","affiliation":[{"name":"From the *Department of Pediatrics, Program in Molecular Medicine, University of Massachusetts Medical Center, Worcester, Massachusetts 01605; and \u2021Therion Biologics Corporation, Cambridge, Massachusetts 02142"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine","family":"Luzuriaga","sequence":"additional","affiliation":[{"name":"From the *Department of Pediatrics, Program in Molecular Medicine, University of Massachusetts Medical Center, Worcester, Massachusetts 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