{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T15:54:51Z","timestamp":1791215691767,"version":"4.1.0"},"reference-count":43,"publisher":"Rockefeller University Press","issue":"5","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1997,8,29]]},"abstract":"<jats:p>Induction and maintenance of cytotoxic T lymphocyte (CTL) activity specific for a primary endogenous tumor was investigated in vivo. The simian virus 40 T antigen (Tag) expressed under the control of the rat insulin promoter (RIP) induced pancreatic \u03b2-cell tumors producing insulin, causing progressive hypoglycemia. As an endogenous tumor antigen, the lymphocytic choriomeningitis virus (LCMV) glycoprotein (GP) was introduced also under the control of the RIP. No significant spontaneous CTL activation against GP was observed. However, LCMV infection induced an antitumor CTL response which efficiently reduced the tumor mass, resulting in temporarily normalized blood glucose levels and prolonged survival of double transgenic RIP(GP \u00d7 Tag2) mice (137 \u00b1 18 d) as opposed to control RIP-Tag2 mice (88 \u00b1 8 d). Surprisingly, the tumor-specific CTL response was not sustained despite the facts that the tumor cells continued to express MHC class I and LCMV-GP\u2013specific CTLs were present and not tolerized. Subsequent adoptive transfer of virus activated spleen cells into RIP(GP \u00d7 Tag2) mice further prolonged survival (168 \u00b1 11 d), demonstrating continued expression of the LCMV-GP tumor antigen and MHC class I. The data show that the tumor did not spontaneously induce or maintain an activated CTL response, revealing a profound lack of immunogenicity in vivo. Therefore, repetitive immunizations are necessary for prolonged antitumor immunotherapy. In addition, the data suggest that the risk for induction of chronic autoimmune diseases is limited, which may encourage immunotherapy against antigens selectively but not exclusively expressed by the tumor.<\/jats:p>","DOI":"10.1084\/jem.186.5.645","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:49:30Z","timestamp":1027702170000},"page":"645-653","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":203,"title":["Self Antigens Expressed by Solid Tumors Do Not Efficiently Stimulate Naive or Activated T Cells: Implications for Immunotherapy"],"prefix":"10.1084","volume":"186","author":[{"given":"Daniel E.","family":"Speiser","sequence":"first","affiliation":[{"name":"From the *Ontario Cancer Institute, Department of Medical Biophysics and Department of Immunology, University of Toronto, Ontario M5G 2M9, Canada; \u2021Department of Biochemistry and Biophysics, University of California, San Francisco, California 94143-0534; and \u00a7Institute for Experimental Immunology, University Hospital of Z\u00fcrich, CH-8091 Z\u00fcrich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renata","family":"Miranda","sequence":"additional","affiliation":[{"name":"From the *Ontario Cancer Institute, Department of Medical Biophysics and Department of Immunology, University of Toronto, Ontario M5G 2M9, Canada; \u2021Department of Biochemistry and Biophysics, University of California, San Francisco, California 94143-0534; and \u00a7Institute for Experimental Immunology, University Hospital of Z\u00fcrich, CH-8091 Z\u00fcrich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arsen","family":"Zakarian","sequence":"additional","affiliation":[{"name":"From the *Ontario Cancer Institute, Department of Medical Biophysics and Department of Immunology, University of Toronto, Ontario M5G 2M9, Canada; \u2021Department of Biochemistry and 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Canada; \u2021Department of Biochemistry and Biophysics, University of California, San Francisco, California 94143-0534; and \u00a7Institute for Experimental Immunology, University Hospital of Z\u00fcrich, CH-8091 Z\u00fcrich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernhard","family":"Odermatt","sequence":"additional","affiliation":[{"name":"From the *Ontario Cancer Institute, Department of Medical Biophysics and Department of Immunology, University of Toronto, Ontario M5G 2M9, Canada; \u2021Department of Biochemistry and Biophysics, University of California, San Francisco, California 94143-0534; and \u00a7Institute for Experimental Immunology, University Hospital of Z\u00fcrich, CH-8091 Z\u00fcrich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas","family":"Hanahan","sequence":"additional","affiliation":[{"name":"From the *Ontario Cancer Institute, Department of Medical Biophysics and Department of Immunology, University of Toronto, Ontario M5G 2M9, Canada; \u2021Department of Biochemistry and Biophysics, University of California, San Francisco, California 94143-0534; and \u00a7Institute for Experimental Immunology, University Hospital of Z\u00fcrich, CH-8091 Z\u00fcrich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rolf M.","family":"Zinkernagel","sequence":"additional","affiliation":[{"name":"From the *Ontario Cancer Institute, Department of Medical Biophysics and Department of Immunology, University of Toronto, Ontario M5G 2M9, Canada; \u2021Department of Biochemistry and Biophysics, University of California, San Francisco, California 94143-0534; and \u00a7Institute for Experimental Immunology, University Hospital of Z\u00fcrich, CH-8091 Z\u00fcrich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamela S.","family":"Ohashi","sequence":"additional","affiliation":[{"name":"From the *Ontario Cancer Institute, Department of 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