{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T07:24:34Z","timestamp":1784013874226,"version":"3.55.0"},"reference-count":48,"publisher":"Rockefeller University Press","issue":"7","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1997,10,6]]},"abstract":"<jats:p>Jaw1 is an endoplasmic reticulum (ER) resident protein representative of a class of proteins post translationally inserted into membranes via a type II membrane anchor (cytosolic NH2 domain, lumenal COOH domain) in a translocon-independent manner. We found that Jaw1 can efficiently deliver a COOH-terminal antigenic peptide to class I molecules in transporter associated with antigen processing (TAP)-deficient cells or cells in which TAP is inactivated by the ICP47 protein. Peptide delivery mediated by Jaw1 to class I molecules was equal or better than that mediated by the adenovirus E3\/19K glycoprotein signal sequence, and was sufficient to enable cytofluorographic detection of newly recruited thermostabile class I molecules at the surface of TAP-deficient cells. Deletion of the transmembrane region retargeted Jaw1 from the ER to the cytosol, and severely, although incompletely, abrogated its TAP-independent peptide carrier activity. Use of different protease inhibitors revealed the involvement of a nonproteasomal protease in the TAP-independent activity of cytosolic Jaw1. These findings demonstrate two novel TAP-independent routes of antigen processing; one based on highly efficient peptide liberation from the COOH terminus of membrane proteins in the ER, the other on delivery of a cytosolic protein to the ER by an unknown route.<\/jats:p>","DOI":"10.1084\/jem.186.7.1087","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:49:30Z","timestamp":1027702170000},"page":"1087-1098","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":71,"title":["Two Novel Routes of Transporter Associated with Antigen Processing (TAP)-independent Major Histocompatibility Complex Class I Antigen Processing"],"prefix":"10.1084","volume":"186","author":[{"given":"Heidi Link","family":"Snyder","sequence":"first","affiliation":[{"name":"From the *Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892-0440; the \u2021Department of Medicine, University of Minnesota, Minneapolis, Minnesota 55455; the \u00a7Electron Microscopy Laboratory, University of Zurich, Zurich, Switzerland CH-8091; and the \u00b6Mount Sinai School of Medicine of the City University of New York, Department of Pharmacology, New York 10029"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor","family":"Ba\u010d\u00edk","sequence":"additional","affiliation":[{"name":"From the *Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892-0440; the \u2021Department of Medicine, University of Minnesota, Minneapolis, Minnesota 55455; the \u00a7Electron Microscopy Laboratory, University of Zurich, Zurich, Switzerland CH-8091; and the \u00b6Mount Sinai School of Medicine of the City University of New York, Department of Pharmacology, New York 10029"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jack R.","family":"Bennink","sequence":"additional","affiliation":[{"name":"From the *Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892-0440; the \u2021Department of Medicine, University of Minnesota, Minneapolis, Minnesota 55455; the \u00a7Electron Microscopy Laboratory, University of Zurich, Zurich, Switzerland CH-8091; and the \u00b6Mount Sinai School of Medicine of the City University of New York, Department of Pharmacology, New York 10029"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grainne","family":"Kearns","sequence":"additional","affiliation":[{"name":"From the *Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892-0440; the \u2021Department of Medicine, University of Minnesota, Minneapolis, Minnesota 55455; the \u00a7Electron Microscopy Laboratory, University of Zurich, Zurich, Switzerland CH-8091; and the \u00b6Mount Sinai School of Medicine of the City University of New York, Department of Pharmacology, New York 10029"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy W.","family":"Behrens","sequence":"additional","affiliation":[{"name":"From the *Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892-0440; the \u2021Department of Medicine, University of Minnesota, Minneapolis, Minnesota 55455; the \u00a7Electron Microscopy Laboratory, University of Zurich, Zurich, Switzerland CH-8091; and the \u00b6Mount Sinai School of Medicine of the City University of New York, Department of Pharmacology, New York 10029"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"B\u00e4chi","sequence":"additional","affiliation":[{"name":"From the *Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892-0440; the \u2021Department of Medicine, University of Minnesota, Minneapolis, Minnesota 55455; the \u00a7Electron Microscopy Laboratory, University of Zurich, Zurich, Switzerland CH-8091; and the \u00b6Mount Sinai School of Medicine of the City University of New York, Department of Pharmacology, New York 10029"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marian","family":"Orlowski","sequence":"additional","affiliation":[{"name":"From the *Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892-0440; the \u2021Department of Medicine, University of Minnesota, Minneapolis, Minnesota 55455; the \u00a7Electron Microscopy Laboratory, University of Zurich, Zurich, Switzerland CH-8091; and the \u00b6Mount Sinai School of Medicine of the City University of New York, Department of Pharmacology, New York 10029"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan W.","family":"Yewdell","sequence":"additional","affiliation":[{"name":"From the *Laboratory of Viral Diseases, National 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