{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T10:11:40Z","timestamp":1770718300113,"version":"3.49.0"},"reference-count":28,"publisher":"Rockefeller University Press","issue":"8","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1997,10,20]]},"abstract":"<jats:p>Pott's disease (spinal tuberculosis), a condition characterized by massive resorption of the spinal vertebrae, is one of the most striking pathologies resulting from local infection with Mycobacterium tuberculosis (Mt; Boachie-Adjei, O., and R.G. Squillante. 1996. Orthop. Clin. North Am. 27:95\u2013103). The pathogenesis of Pott's disease is not established. Here we report for the first time that a protein, identified by a monoclonal antibody to be the Mt heat shock protein (Baird, P.N., L.M. Hall, and A.R.M. Coates. 1989. J. Gen. Microbiol. 135:931\u2013939) chaperonin (cpn) 10, is responsible for the osteolytic activity of this bacterium. Recombinant Mt cpn10 is a potent stimulator of bone resorption in bone explant cultures and induces osteoclast recruitment, while inhibiting the proliferation of an osteoblast bone\u2013forming cell line. Furthermore, we have found that synthetic peptides corresponding to sequences within the flexible loop and sequence 65\u201370 of Mt cpn10 may comprise a single conformational unit which encompasses its potent bone-resorbing activity. Our findings suggest that Mt cpn10 may be a valuable pharmacological target for the clinical therapy of vertebral tuberculosis and possibly other bone diseases.<\/jats:p>","DOI":"10.1084\/jem.186.8.1241","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:49:30Z","timestamp":1027702170000},"page":"1241-1246","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":71,"title":["<i>Mycobacterium tuberculosis <\/i>Chaperonin 10 Stimulates Bone Resorption: A Potential Contributory Factor in Pott's Disease"],"prefix":"10.1084","volume":"186","author":[{"given":"Sajeda","family":"Meghji","sequence":"first","affiliation":[{"name":"From the *Maxillofacial Surgery Research Unit, Eastman Dental Institute, University College London, London WC1X 8LD, United Kingdom; \u2021Italfarmaco SpA, Centro Richerche, 20092 Cinisello B (MI), Italy; \u00a7Molecular Immunology Division, National Institute for Medical Research, London NW7 1AA, United Kingdom; \u2016Howard Hughes Medical Institute, The University of Texas, Southwestern Medical Center, Dallas, Texas 75235; and \u00b6Division of Molecular Microbiology, St. George's Hospital Medical School, London SW17 0RE, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter A.","family":"White","sequence":"additional","affiliation":[{"name":"From the *Maxillofacial Surgery Research Unit, Eastman Dental Institute, University College London, London WC1X 8LD, United Kingdom; \u2021Italfarmaco SpA, Centro Richerche, 20092 Cinisello B (MI), Italy; \u00a7Molecular Immunology Division, National Institute for Medical Research, London NW7 1AA, United Kingdom; \u2016Howard Hughes Medical Institute, The University of Texas, Southwestern Medical Center, Dallas, Texas 75235; and \u00b6Division of Molecular Microbiology, St. George's Hospital Medical School, London SW17 0RE, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sean P.","family":"Nair","sequence":"additional","affiliation":[{"name":"From the *Maxillofacial Surgery Research Unit, Eastman Dental Institute, University College London, London WC1X 8LD, United Kingdom; \u2021Italfarmaco SpA, Centro Richerche, 20092 Cinisello B (MI), Italy; \u00a7Molecular Immunology Division, National Institute for Medical Research, London NW7 1AA, United Kingdom; \u2016Howard Hughes Medical Institute, The University of Texas, Southwestern Medical Center, Dallas, Texas 75235; and \u00b6Division of Molecular Microbiology, St. George's Hospital Medical School, London SW17 0RE, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krisanavane","family":"Reddi","sequence":"additional","affiliation":[{"name":"From the *Maxillofacial Surgery Research Unit, Eastman Dental Institute, University College London, London WC1X 8LD, United Kingdom; \u2021Italfarmaco SpA, Centro Richerche, 20092 Cinisello B (MI), Italy; \u00a7Molecular Immunology Division, National Institute for Medical Research, London NW7 1AA, United Kingdom; \u2016Howard Hughes Medical Institute, The University of Texas, Southwestern Medical Center, Dallas, Texas 75235; and \u00b6Division of Molecular Microbiology, St. George's Hospital Medical School, London SW17 0RE, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyle","family":"Heron","sequence":"additional","affiliation":[{"name":"From the *Maxillofacial Surgery Research Unit, Eastman Dental Institute, University College London, London WC1X 8LD, United Kingdom; \u2021Italfarmaco SpA, Centro Richerche, 20092 Cinisello B (MI), Italy; \u00a7Molecular Immunology Division, National Institute for Medical Research, London NW7 1AA, United Kingdom; \u2016Howard Hughes Medical Institute, The University of Texas, Southwestern Medical Center, Dallas, Texas 75235; and \u00b6Division of Molecular Microbiology, St. George's Hospital Medical School, London SW17 0RE, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian","family":"Henderson","sequence":"additional","affiliation":[{"name":"From the *Maxillofacial Surgery Research Unit, Eastman Dental Institute, University College London, London WC1X 8LD, United Kingdom; \u2021Italfarmaco SpA, Centro Richerche, 20092 Cinisello B (MI), Italy; \u00a7Molecular Immunology Division, National Institute for Medical Research, London NW7 1AA, United Kingdom; \u2016Howard Hughes Medical Institute, The University of Texas, Southwestern Medical Center, Dallas, Texas 75235; and \u00b6Division of Molecular Microbiology, St. George's Hospital Medical School, London SW17 0RE, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Zaliani","sequence":"additional","affiliation":[{"name":"From the *Maxillofacial Surgery Research Unit, Eastman Dental Institute, University College London, London WC1X 8LD, United Kingdom; \u2021Italfarmaco SpA, Centro Richerche, 20092 Cinisello B (MI), Italy; \u00a7Molecular Immunology Division, National Institute for Medical Research, London NW7 1AA, United Kingdom; \u2016Howard Hughes Medical Institute, The University of Texas, Southwestern Medical Center, Dallas, Texas 75235; and \u00b6Division of Molecular Microbiology, St. George's Hospital Medical School, London SW17 0RE, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gianluca","family":"Fossati","sequence":"additional","affiliation":[{"name":"From the *Maxillofacial Surgery Research Unit, Eastman Dental Institute, University College London, London WC1X 8LD, United Kingdom; \u2021Italfarmaco SpA, Centro Richerche, 20092 Cinisello B (MI), Italy; \u00a7Molecular Immunology Division, National Institute for Medical Research, London NW7 1AA, United Kingdom; \u2016Howard Hughes Medical Institute, The University of Texas, Southwestern Medical Center, Dallas, Texas 75235; and \u00b6Division of Molecular Microbiology, St. George's Hospital Medical School, London SW17 0RE, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paolo","family":"Mascagni","sequence":"additional","affiliation":[{"name":"From the *Maxillofacial Surgery Research Unit, Eastman Dental Institute, University College London, London WC1X 8LD, United Kingdom; \u2021Italfarmaco SpA, Centro Richerche, 20092 Cinisello B (MI), Italy; \u00a7Molecular Immunology Division, National Institute for Medical Research, London NW7 1AA, United Kingdom; \u2016Howard Hughes Medical Institute, The University of Texas, Southwestern Medical Center, Dallas, Texas 75235; and \u00b6Division of Molecular Microbiology, St. George's Hospital Medical School, London SW17 0RE, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John F.","family":"Hunt","sequence":"additional","affiliation":[{"name":"From the *Maxillofacial Surgery Research Unit, Eastman Dental Institute, University College London, London WC1X 8LD, United Kingdom; \u2021Italfarmaco SpA, Centro Richerche, 20092 Cinisello B (MI), Italy; \u00a7Molecular Immunology Division, National Institute for Medical Research, London NW7 1AA, United Kingdom; \u2016Howard Hughes Medical Institute, The University of Texas, Southwestern Medical Center, Dallas, Texas 75235; and \u00b6Division of Molecular Microbiology, St. George's Hospital Medical School, London SW17 0RE, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael M.","family":"Roberts","sequence":"additional","affiliation":[{"name":"From the *Maxillofacial Surgery Research Unit, Eastman Dental Institute, University College London, London WC1X 8LD, United Kingdom; \u2021Italfarmaco SpA, Centro Richerche, 20092 Cinisello B (MI), Italy; \u00a7Molecular Immunology Division, National Institute for Medical Research, London NW7 1AA, United Kingdom; \u2016Howard Hughes Medical Institute, The University of Texas, Southwestern Medical Center, Dallas, Texas 75235; and \u00b6Division of Molecular Microbiology, St. George's Hospital Medical School, London SW17 0RE, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony R.M.","family":"Coates","sequence":"additional","affiliation":[{"name":"From the *Maxillofacial Surgery Research Unit, Eastman Dental Institute, University College London, London WC1X 8LD, United Kingdom; \u2021Italfarmaco SpA, Centro Richerche, 20092 Cinisello B (MI), Italy; \u00a7Molecular Immunology Division, National Institute for Medical Research, London NW7 1AA, United Kingdom; \u2016Howard Hughes Medical Institute, The University of Texas, Southwestern Medical Center, Dallas, Texas 75235; and \u00b6Division of Molecular Microbiology, St. George's Hospital Medical School, London SW17 0RE, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1997,10,20]]},"reference":[{"key":"2023072507450624500_B1","first-page":"6","article-title":"Tuberculosis in developing countries: burden, intervention and cost","volume":"65","author":"Murray","year":"1990","journal-title":"Bull Int Union Tuberc Lung Dis"},{"key":"2023072507450624500_B2","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/S0030-5898(20)32054-X","article-title":"Tuberculosis of the spine","volume":"27","author":"Boachie-Adjei","year":"1996","journal-title":"Orthop Clin North Am"},{"key":"2023072507450624500_B3","doi-asserted-by":"crossref","first-page":"2371","DOI":"10.1128\/iai.64.7.2371-2380.1996","article-title":"Bacterially induced bone destruction: mechanisms and misconceptions","volume":"64","author":"Nair","year":"1996","journal-title":"Infect 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