{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T14:29:26Z","timestamp":1792765766877,"version":"4.5.0"},"reference-count":34,"publisher":"Rockefeller University Press","issue":"8","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1997,10,20]]},"abstract":"<jats:p>To determine the role of CD11\/CD18 complexes in neutrophil emigration, inflammation was induced in the skin, lungs, or peritoneum of mutant mice deficient in CD18 (CD18\u2212\/\u2212 mutants). Peripheral blood of CD18\u2212\/\u2212 mutants contained 11-fold more neutrophils than did blood of wild-type (WT) mice. During irritant dermatitis induced by topical application of croton oil, the number of emigrated neutrophils in histological sections of dermis was 98% less in CD18\u2212\/\u2212 mutants than in WT mice. During Streptococcus pneumoniae pneumonia, neutrophil emigration in CD18\u2212\/\u2212 mutants was not reduced. These data are consistent with expectations based on studies using blocking antibodies to inhibit CD11\/CD18 complexes, and on observations of humans lacking CD11\/CD18 complexes. The number of emigrated neutrophils in lung sections during Escherichia coli pneumonia, or in peritoneal lavage fluid after 4 h of S. pneumoniae peritonitis, was not reduced in CD18\u2212\/\u2212 mutants, but rather was greater than the WT values (240 \u00b1 30 and 220 \u00b1 30% WT, respectively). Also, there was no inhibition of neutrophil emigration during sterile peritonitis induced by intraperitoneal injection of thioglycollate (90 \u00b1 20% WT). These data contrast with expectations. Whereas CD11\/CD18 complexes are essential to the dermal emigration of neutrophils during acute dermatitis, CD18\u2212\/\u2212 mutant mice demonstrate surprising alternative pathways for neutrophil emigration during pneumonia or peritonitis.<\/jats:p>","DOI":"10.1084\/jem.186.8.1357","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:49:30Z","timestamp":1027702170000},"page":"1357-1364","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":209,"title":["Neutrophil Emigration in the Skin, Lungs, and Peritoneum: Different Requirements for CD11\/CD18 Revealed by CD18-deficient Mice"],"prefix":"10.1084","volume":"186","author":[{"given":"Joseph P.","family":"Mizgerd","sequence":"first","affiliation":[{"name":"From the *Physiology Program, Harvard School of Public Health, Boston, Massachusetts 02115; and \u2021Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Kubo","sequence":"additional","affiliation":[{"name":"From the *Physiology Program, Harvard School of Public Health, Boston, Massachusetts 02115; and \u2021Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory J.","family":"Kutkoski","sequence":"additional","affiliation":[{"name":"From the *Physiology Program, Harvard School of Public Health, Boston, Massachusetts 02115; and \u2021Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina D.","family":"Bhagwan","sequence":"additional","affiliation":[{"name":"From the *Physiology Program, Harvard School of Public Health, Boston, Massachusetts 02115; and \u2021Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin","family":"Scharffetter-Kochanek","sequence":"additional","affiliation":[{"name":"From the *Physiology Program, Harvard School of Public Health, Boston, Massachusetts 02115; and \u2021Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arthur L.","family":"Beaudet","sequence":"additional","affiliation":[{"name":"From the *Physiology Program, Harvard School of Public Health, Boston, Massachusetts 02115; and \u2021Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire M.","family":"Doerschuk","sequence":"additional","affiliation":[{"name":"From the *Physiology Program, Harvard School of Public Health, Boston, Massachusetts 02115; and \u2021Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1997,10,20]]},"reference":[{"key":"2023072507355119900_B1","doi-asserted-by":"crossref","first-page":"2068","DOI":"10.1182\/blood.V84.7.2068.2068","article-title":"Leukocyte-endothelial adhesion molecules","volume":"84","author":"Carlos","year":"1994","journal-title":"Blood"},{"key":"2023072507355119900_B2","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1182\/blood.V69.1.338.338","article-title":"A monoclonal antibody to the membrane glycoprotein complex CD18 inhibits polymorphonuclear leukocyte accumulation and plasma leakage in vivo","volume":"69","author":"Arfors","year":"1987","journal-title":"Blood"},{"key":"2023072507355119900_B3","doi-asserted-by":"crossref","first-page":"2327","DOI":"10.4049\/jimmunol.144.6.2327","article-title":"CD18-dependent and -independent mechanisms of neutrophil adherence in the pulmonary and systemic microvasculature of rabbits","volume":"114","author":"Doerschuk","year":"1990","journal-title":"J Immunol"},{"key":"2023072507355119900_B4","doi-asserted-by":"crossref","first-page":"932","DOI":"10.1016\/S0022-3476(82)80013-9","article-title":"Severe recurrent bacterial infections associated with defective adherence and chemotaxis in two patients with neutrophils deficient in a cell-associated glycoprotein","volume":"101","author":"Bowen","year":"1982","journal-title":"J Pediatr"},{"key":"2023072507355119900_B5","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1093\/infdis\/152.4.668","article-title":"The severe and moderate phenotypes of heritable Mac-1, LFA-1 deficiency: their quantitative definition and relation to leukocyte dysfunction and clinical features","volume":"152","author":"Anderson","year":"1985","journal-title":"J Infect Dis"},{"key":"2023072507355119900_B6","doi-asserted-by":"crossref","first-page":"1382","DOI":"10.1182\/blood.V65.6.1382.bloodjournal6561382","article-title":"Abnormal adherence-related functions of neutrophils, monocytes, and Epstein-Barr virus-transformed B cells in a patient with C3bi receptor deficiency","volume":"65","author":"Buescher","year":"1985","journal-title":"Blood"},{"key":"2023072507355119900_B7","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1182\/blood.V65.3.696.696","article-title":"Glycoprotein-180 deficiency: genetics and abnormal neutrophil activation","volume":"65","author":"Weisman","year":"1985","journal-title":"Blood"},{"key":"2023072507355119900_B8","doi-asserted-by":"crossref","first-page":"119","DOI":"10.3109\/15513819209023288","article-title":"Leukocyte adhesion deficiency: clinical and postmortem observations","volume":"12","author":"Hawkins","year":"1992","journal-title":"Pediatr Pathol"},{"key":"2023072507355119900_B9","doi-asserted-by":"crossref","first-page":"2407","DOI":"10.4049\/jimmunol.150.6.2407","article-title":"Role of \u03b21, \u03b22 integrins, and ICAM-1 in lung injury after deposition of IgG and IgA immune complexes","volume":"150","author":"Mulligan","year":"1993","journal-title":"J Immunol"},{"key":"2023072507355119900_B10","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1165\/ajrcmb.10.4.7510985","article-title":"Disparate roles of the \u03b22-integrin CD18 in the local accumulation of neutrophils in pulmonary and cutaneous inflammation in the rabbit","volume":"10","author":"Hellewell","year":"1994","journal-title":"Am J Respir Cell Mol Biol"},{"key":"2023072507355119900_B11","first-page":"1297","article-title":"Role of CD11\/CD18 in neutrophil emigration during acute and recurrent Pseudomonas aeruginosa-induced pneumonia in rabbits","volume":"148","author":"Kumasaka","year":"1996","journal-title":"Am J Pathol"},{"key":"2023072507355119900_B12","doi-asserted-by":"crossref","first-page":"5016","DOI":"10.4049\/jimmunol.157.11.5016","article-title":"The roles of CD11\/CD18 and ICAM-1 in acute Pseudomonas aeruginosa-induced pneumonia in mice","volume":"157","author":"Qin","year":"1996","journal-title":"J Immunol"},{"key":"2023072507355119900_B13","doi-asserted-by":"crossref","first-page":"1571","DOI":"10.4049\/jimmunol.151.3.1571","article-title":"Gene targeting yields a CD18-mutant mouse for study of inflammation","volume":"151","author":"Wilson","year":"1993","journal-title":"J Immunol"},{"key":"2023072507355119900_B14","doi-asserted-by":"crossref","first-page":"8529","DOI":"10.1073\/pnas.90.18.8529","article-title":"Inflammatory and immune responses are impaired in mice deficient in intercellular adhesion molecule 1","volume":"90","author":"Sligh","year":"1993","journal-title":"Proc Nat Acad Sci USA"},{"key":"2023072507355119900_B15","doi-asserted-by":"crossref","first-page":"1782","DOI":"10.1172\/JCI117856","article-title":"P-selectin\/ICAM-1 double mutant mice: acute emigration of neutrophils into the peritoneum is completely absent but is normal into pulmonary alveoli","volume":"95","author":"Bullard","year":"1995","journal-title":"J Clin Invest"},{"key":"2023072507355119900_B16","doi-asserted-by":"crossref","first-page":"2329","DOI":"10.1084\/jem.183.5.2329","article-title":"Infections and deficiency of leukocyte rolling and recruitment in E-\/P-selectin mutant mice","volume":"183","author":"Bullard","year":"1996","journal-title":"J Exp Med"},{"key":"2023072507355119900_B17","first-page":"809","article-title":"Generation and characterization of a CD18 complete knock-out mouse\u2014new insights in the function of the CD18 molecule?","volume":"106","author":"Scharffetter-Kochanek","year":"1996","journal-title":"J Investig Dermatol"},{"key":"2023072507355119900_B18"},{"key":"2023072507355119900_B19","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1084\/jem.184.2.639","article-title":"Selectins and neutrophil traffic: margination and Streptococcus pneumoniae-induced emigration in murine lungs","volume":"184","author":"Mizgerd","year":"1996","journal-title":"J Exp Med"},{"key":"2023072507355119900_B20","doi-asserted-by":"crossref","first-page":"2702","DOI":"10.1128\/iai.62.7.2702-2706.1994","article-title":"Listeria monocytogenes, but not Salmonella typhimurium, elicits a CD18-independent mechanism of neutrophil extravasation into the murine peritoneal cavity","volume":"62","author":"Conlan","year":"1994","journal-title":"Infect Immunol"},{"key":"2023072507355119900_B21","doi-asserted-by":"crossref","first-page":"1415","DOI":"10.1084\/jem.183.4.1415","article-title":"LFA-1\u2013deficient mice show normal CTL responses to virus, but fail to reject immunogenic tumor","volume":"183","author":"Schmits","year":"1996","journal-title":"J Exp Med"},{"key":"2023072507355119900_B22","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1016\/S1074-7613(00)80278-2","article-title":"A novel role for the \u03b22 integrin CD11b\/CD18 in neutrophil apoptosis: a homeostatic mechanism in inflammation","volume":"5","author":"Coxon","year":"1996","journal-title":"Immunity"},{"key":"2023072507355119900_B23","doi-asserted-by":"crossref","first-page":"1340","DOI":"10.1172\/JCI119293","article-title":"LFA-1 is sufficient in mediating neutrophil emigration in Mac-1 deficient mice","volume":"99","author":"Lu","year":"1997","journal-title":"J Clin Invest"},{"key":"2023072507355119900_B24","doi-asserted-by":"crossref","first-page":"1168","DOI":"10.1172\/JCI116686","article-title":"CD18-independent neutrophil and mononuclear leukocyte emigration into the peritoneum of rabbits","volume":"92","author":"Winn","year":"1993","journal-title":"J Clin Invest"},{"key":"2023072507355119900_B25","first-page":"259","article-title":"Streptococcus pneumoniae-stimulated macrophages induce neutrophils to emigrate by a CD18-independent mechanism of adherence","volume":"31","author":"Mileski","year":"1990","journal-title":"Circ Shock"},{"key":"2023072507355119900_B26","doi-asserted-by":"crossref","first-page":"2175","DOI":"10.1084\/jem.183.5.2175","article-title":"Rat blood neutrophils express very late antigen 4 and it mediates migration to arthritic joint and dermal inflammation","volume":"183","author":"Issekutz","year":"1996","journal-title":"J Exp Med"},{"key":"2023072507355119900_B27","doi-asserted-by":"crossref","first-page":"1100","DOI":"10.1038\/ki.1994.372","article-title":"Mechanisms of polymorphonuclear leukocyte mediated peritoneal mesothelial cell injury","volume":"46","author":"Andreoli","year":"1994","journal-title":"Kidney Int"},{"key":"2023072507355119900_B28","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1681\/ASN.V72208","article-title":"Adherence of neutrophils to human peritoneal mesothelial cells- role of intercellular adhesion molecule-1","volume":"7","author":"Liberek","year":"1996","journal-title":"J Am Soc Nephrol"},{"key":"2023072507355119900_B29","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1093\/infdis\/164.5.901","article-title":"Granulocyte colony-stimulating factor enhances pulmonary host defenses in normal and ethanol-treated rats","volume":"164","author":"Nelson","year":"1991","journal-title":"J Infect Dis"},{"key":"2023072507355119900_B30","doi-asserted-by":"crossref","first-page":"922","DOI":"10.1093\/infdis\/168.4.922","article-title":"Granulocyte colony-stimulating factor protects control rats but not ethanol-fed rats from fatal pneumococcal pneumonia","volume":"168","author":"Lister","year":"1993","journal-title":"J Infect Dis"},{"key":"2023072507355119900_B31","doi-asserted-by":"crossref","first-page":"2066","DOI":"10.1152\/jappl.1996.80.6.2066","article-title":"Controlled trials of rG-CSF and CD11b-directed MAb during hyperoxia and E. colipneumonia in rats","volume":"80","author":"Freeman","year":"1996","journal-title":"J Appl Physiol"},{"key":"2023072507355119900_B32","first-page":"475","article-title":"Effect of recombinant human granulocyte colony-stimulating factor (rhG-CSF) on murine resistance against Listeria monocytogenes.","volume":"75","author":"Serushago","year":"1992","journal-title":"Immunology"},{"key":"2023072507355119900_B33","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1097\/00024382-199507000-00011","article-title":"Granulocyte colony-stimulating factor improves myelopoiesis and host defense in fulminant intra-abdominal sepsis in rats","volume":"4","author":"Lundblad","year":"1995","journal-title":"Shock"},{"key":"2023072507355119900_B34","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1006\/jsre.1996.0128","article-title":"Effects of granulocyte colony stimulating factor in a nonneutropenic rodent model of Escherichia coliperitonitis","volume":"61","author":"Dunne","year":"1996","journal-title":"J Surg Res"}],"container-title":["The Journal of Experimental Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/186\/8\/1357\/1685573\/97-0984.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/186\/8\/1357\/1685573\/97-0984.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T08:18:45Z","timestamp":1690273125000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/186\/8\/1357\/7320\/Neutrophil-Emigration-in-the-Skin-Lungs-and"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,10,20]]},"references-count":34,"journal-issue":{"issue":"8","published-print":{"date-parts":[[1997,10,20]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.186.8.1357","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,10,20]]}}}