{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T15:48:42Z","timestamp":1770738522900,"version":"3.49.0"},"reference-count":48,"publisher":"Rockefeller University Press","issue":"10","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1998,5,18]]},"abstract":"<jats:p>Protective immunity to infection by many intracellular pathogens requires recognition by cytotoxic T lymphocytes (CTLs) of antigens presented on major histocompatibility complex (MHC) class I molecules. To be presented for recognition by pathogen-specific CTLs, these antigens must gain access to the host cell class I processing pathway. In the case of intracellular bacterial pathogens, the majority of bacterial proteins are retained within the bacterial membrane and therefore remain inaccessible to the host cell for antigen processing. We have isolated a CTL clone from a C57BL\/6 mouse infected with the intracellular gram-positive bacterium Listeria monocytogenes (LM) and have identified the source of the antigen. Using a genomic expression library, we determined that the clone recognizes an antigenic N-formyl peptide presented by the nonpolymorphic murine MHC class Ib molecule, H2-M3. Several lengths of this peptide were able to sensitize cells for lysis by this CTL clone. The source of this antigenic peptide is a 23\u2013amino acid polypeptide encoded at the start of a polycistronic region. Analysis of mRNA secondary structure of this region suggests that this polypeptide may be a leader peptide encoded by a transcriptional attenuator.<\/jats:p>","DOI":"10.1084\/jem.187.10.1711","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:49:30Z","timestamp":1027702170000},"page":"1711-1719","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":48,"title":["H2-M3 Restricted Presentation of a <i>Listeria<\/i>-derived Leader Peptide"],"prefix":"10.1084","volume":"187","author":[{"given":"Michael F.","family":"Princiotta","sequence":"first","affiliation":[{"name":"From the *National Jewish Medical and Research Center and \u2021Department of Immunology, University of Colorado Health Sciences Center, Denver, Colorado 80206; and the \u2021Howard Hughes Medical Institute and Department of Immunology, University of Washington, Seattle, Washington 98195"},{"name":"From the *National Jewish Medical and Research Center and \u2021Department of Immunology, University of Colorado Health Sciences Center, Denver, Colorado 80206; and the \u2021Howard Hughes Medical Institute and Department of Immunology, University of Washington, Seattle, Washington 98195"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laurel L.","family":"Lenz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael J.","family":"Bevan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Uwe D.","family":"Staerz","sequence":"additional","affiliation":[{"name":"From the *National Jewish Medical and Research Center and \u2021Department of Immunology, University of Colorado Health Sciences Center, Denver, Colorado 80206; and the \u2021Howard Hughes Medical Institute and Department of Immunology, University of Washington, Seattle, Washington 98195"},{"name":"From the *National Jewish Medical and Research Center and \u2021Department of Immunology, University of Colorado Health Sciences Center, Denver, Colorado 80206; and the \u2021Howard Hughes Medical Institute and Department of Immunology, University of Washington, Seattle, Washington 98195"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1998,5,18]]},"reference":[{"key":"2023072507450529200_B1","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1111\/j.1600-065X.1997.tb00988.x","article-title":"Studies in listeriosis show the strong symbiosis between the innate cellular system and the T-cell response","volume":"158","author":"Unanue","year":"1997","journal-title":"Immunol Rev"},{"key":"2023072507450529200_B2","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.1084\/jem.135.5.1104","article-title":"Requirement of thymus (T) lymphocytes for resistance to listeriosis","volume":"135","author":"Lane","year":"1972","journal-title":"J Exp Med"},{"key":"2023072507450529200_B3","doi-asserted-by":"crossref","first-page":"2688","DOI":"10.4049\/jimmunol.137.8.2688","article-title":"Antigen-specific Lyt-2+ cytolytic T lymphocytes from mice infected with the intracellular bacterium Listeria monocytogenes.","volume":"137","author":"De Libero","year":"1986","journal-title":"J Immunol"},{"key":"2023072507450529200_B4","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1016\/0952-7915(93)90028-Q","article-title":"Cellular immunity to intracellular bacteria","volume":"5","author":"Pamer","year":"1993","journal-title":"Curr Opin Immunol"},{"key":"2023072507450529200_B5","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/0952-7915(94)90031-0","article-title":"Antigen presentation by non-classical class I molecules","volume":"6","author":"Stroynowski","year":"1994","journal-title":"Curr Opin Immunol"},{"key":"2023072507450529200_B6","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1126\/science.1496381","article-title":"Specialized role for a murine class I-b MHC molecule in prokaryotic host defenses","volume":"257","author":"Kurlander","year":"1992","journal-title":"Science"},{"key":"2023072507450529200_B7","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1016\/S0952-7915(97)80095-7","article-title":"The role of CD1 molecules in immune responses to infection","volume":"9","author":"Maher","year":"1997","journal-title":"Curr Opin Immunol"},{"key":"2023072507450529200_B8","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1038\/372691a0","article-title":"Recognition of a lipid antigen by CD1-restricted alpha beta+ T cells","volume":"372","author":"Beckman","year":"1994","journal-title":"Nature"},{"key":"2023072507450529200_B9","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1126\/science.7542404","article-title":"CD1-restricted T cell recognition of microbial lipoglycan antigens","volume":"269","author":"Sieling","year":"1995","journal-title":"Science"},{"key":"2023072507450529200_B10","doi-asserted-by":"crossref","first-page":"201","DOI":"10.4049\/jimmunol.151.1.201","article-title":"Differential amino-terminal anchors for peptide binding to H-2M3a or H-2Kb and H-2Db","volume":"151","author":"Shawar","year":"1993","journal-title":"J Immunol"},{"key":"2023072507450529200_B11","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1084\/jem.171.3.897","article-title":"Specialized functions of MHC class I molecules. 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