{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T17:36:27Z","timestamp":1790357787823,"version":"4.1.0"},"reference-count":35,"publisher":"Rockefeller University Press","issue":"11","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1998,6,1]]},"abstract":"<jats:p>In a murine model of respiratory syncytial virus disease, prior sensitization to the attachment glycoprotein (G) leads to pulmonary eosinophilia and enhanced illness. Three different approaches were taken to dissect the region of G responsible for enhanced disease and protection against challenge. First, mutant viruses, containing frameshifts that altered the COOH terminus of the G protein, were used to challenge mice sensitized by scarification with recombinant vaccinia virus (rVV) expressing wild-type G. Second, cDNA expressing these mutated G proteins were expressed by rVV and used to vaccinate mice before challenge with wild-type respiratory syncytial virus (RSV). These studies identified residues 193\u2013205 to be responsible for G-induced weight loss and lung eosinophilia and showed that this region was not was not necessary for induction of protective immunity. Third, mice were sensitized using an rVV that expressed only amino acids 124\u2013203 of the G protein. Upon RSV challenge, mice sensitized with this rVV developed enhanced weight loss and eosinophilia. This is the first time that a region within RSV (amino acids 193\u2013203) has been shown to be responsible for induction of lung eosinophilia and disease enhancement. Moreover, we now show that it is possible to induce protective immunity with an altered G protein without inducing a pathological response.<\/jats:p>","DOI":"10.1084\/jem.187.11.1921","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:49:30Z","timestamp":1027702170000},"page":"1921-1926","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":115,"title":["Eliminating a Region of Respiratory Syncytial Virus Attachment Protein Allows Induction of Protective Immunity without Vaccine-enhanced Lung Eosinophilia"],"prefix":"10.1084","volume":"187","author":[{"given":"Tim E.","family":"Sparer","sequence":"first","affiliation":[{"name":"From the *Imperial College School of Medicine, National Heart and Lung Institute at St. Mary's, London, W2 1PG United Kingdom; and the \u2021Centro Nacional de Biolog\u00eca Celular y Retrovirus, Instituto de Salud \u2018Carlos III', Majadahonda, 28220, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"Matthews","sequence":"additional","affiliation":[{"name":"From the *Imperial College School of Medicine, National Heart and Lung Institute at St. Mary's, London, W2 1PG United Kingdom; and the \u2021Centro Nacional de Biolog\u00eca Celular y Retrovirus, Instituto de Salud \u2018Carlos III', Majadahonda, 28220, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tracy","family":"Hussell","sequence":"additional","affiliation":[{"name":"From the *Imperial College School of Medicine, National Heart and Lung Institute at St. Mary's, London, W2 1PG United Kingdom; and the \u2021Centro Nacional de Biolog\u00eca Celular y Retrovirus, Instituto de Salud \u2018Carlos III', Majadahonda, 28220, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron J.","family":"Rae","sequence":"additional","affiliation":[{"name":"From the *Imperial College School of Medicine, National Heart and Lung Institute at St. Mary's, London, W2 1PG United Kingdom; and the \u2021Centro Nacional de Biolog\u00eca Celular y Retrovirus, Instituto de Salud \u2018Carlos III', Majadahonda, 28220, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blanca","family":"Garcia-Barreno","sequence":"additional","affiliation":[{"name":"From the *Imperial College School of Medicine, National Heart and Lung Institute at St. Mary's, London, W2 1PG United Kingdom; and the \u2021Centro Nacional de Biolog\u00eca Celular y Retrovirus, Instituto de Salud \u2018Carlos III', Majadahonda, 28220, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose A.","family":"Melero","sequence":"additional","affiliation":[{"name":"From the *Imperial College School of Medicine, National Heart and Lung Institute at St. Mary's, London, W2 1PG United Kingdom; and the \u2021Centro Nacional de Biolog\u00eca Celular y Retrovirus, Instituto de Salud \u2018Carlos III', Majadahonda, 28220, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter J.M.","family":"Openshaw","sequence":"additional","affiliation":[{"name":"From the *Imperial College School of Medicine, National Heart and Lung Institute at St. Mary's, London, W2 1PG United Kingdom; and the \u2021Centro Nacional de Biolog\u00eca Celular y Retrovirus, Instituto de Salud \u2018Carlos III', Majadahonda, 28220, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1998,6,1]]},"reference":[{"key":"2023072507420420700_B1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/infdis\/171.1.1","article-title":"Protective and disease-enhancing immune responses to respiratory syncytial virus","volume":"171","author":"Anderson","year":"1995","journal-title":"J Infect Dis"},{"key":"2023072507420420700_B2","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1007\/BF00196165","article-title":"Immunopathological mechanisms in respiratory syncytial virus disease","volume":"17","author":"Openshaw","year":"1995","journal-title":"Semin Immunopathol"},{"key":"2023072507420420700_B3","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1542\/peds.95.4.500","article-title":"Asthma and immunoglobulin E antibodies after respiratory syncytial virus bronchiolitis: a prospective cohort study with matched controls","volume":"95","author":"Sigurs","year":"1995","journal-title":"Pediatrics"},{"key":"2023072507420420700_B4","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1093\/oxfordjournals.aje.a120955","article-title":"Respiratory syncytial virus disease in infants despite prior administration of antigenic inactivated vaccine","volume":"89","author":"Kim","year":"1969","journal-title":"Am J Epidemiol"},{"key":"2023072507420420700_B5","first-page":"436","article-title":"Respiratory virus immunization. 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