{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T06:03:43Z","timestamp":1791180223572,"version":"4.1.0"},"reference-count":39,"publisher":"Rockefeller University Press","issue":"4","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1998,2,16]]},"abstract":"<jats:p>Anti\u2013neutrophil cytoplasmic antibodies (ANCAs) targeting proteinase 3 (PR3) have a high specifity for Wegener's granulomatosis (WG), and their role in activating leukocytes is well appreciated. In this study, we investigated the influence of PR3-ANCA and murine monoclonal antibodies on human umbilical vascular endothelial cells (HUVECs). Priming of HUVECs with tumor necrosis factor \u03b1 induced endothelial upregulation of PR3 message and surface expression of this antigen, as measured by Cyto-ELISA, with a maximum occurrence after 2 h. Primed cells responded to low concentrations of both antibodies (25 ng\u20132.5 \u03bcg\/ml), but not to control immunoglobulins, with pronounced, dose-dependent phosphoinositide hydrolysis, as assessed by accumulation of inositol phosphates. The signaling response peaked after 20 min, in parallel with the appearance of marked prostacyclin and platelet-activating factor synthesis. The F(ab)2 fragment of ANCA was equally potent as ANCA itself. Disrupture of the endothelial F-actin content by botulinum C2 toxin to avoid antigen\u2013antibody internalization did not affect the response. In addition to the metabolic events, anti-PR3 challenge, in the absence of plasma components, provoked delayed, dose-dependent increase in transendothelial protein leakage. We conclude that anti-PR3 antibodies are potent inductors of the preformed phosphoinositide hydrolysis\u2013related signal tranduction pathway in human endothelial cells. Associated metabolic events and the loss of endothelial barrier properties suggest that anti-PR3\u2013induced activation of endothelial cells may contribute to the pathogenetic sequelae of autoimmune vasculitis characterizing WG.<\/jats:p>","DOI":"10.1084\/jem.187.4.497","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:49:30Z","timestamp":1027702170000},"page":"497-503","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":55,"title":["Wegener's Granulomatosis: Anti\u2013proteinase 3 Antibodies Are Potent Inductors of Human Endothelial Cell Signaling and Leakage Response"],"prefix":"10.1084","volume":"187","author":[{"given":"Ulf","family":"Sibelius","sequence":"first","affiliation":[{"name":"From the *Department of Internal Medicine, Justus-Liebig-University, D-35392 Giessen, Germany; the \u2021Institute of Physiology, Justus-Liebig-University, D-35392 Giessen, Germany; the \u00a7Department of Rheumatology of the Medical University of L\u00fcbeck, 23538 L\u00fcbeck, Germany; and the \u2016First Medical Department, University of Mainz, 55131 Mainz, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katja","family":"Hattar","sequence":"additional","affiliation":[{"name":"From the *Department of Internal Medicine, Justus-Liebig-University, D-35392 Giessen, Germany; the \u2021Institute of Physiology, Justus-Liebig-University, D-35392 Giessen, Germany; the \u00a7Department of Rheumatology of the Medical University of L\u00fcbeck, 23538 L\u00fcbeck, Germany; and the \u2016First Medical Department, University of Mainz, 55131 Mainz, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelika","family":"Schenkel","sequence":"additional","affiliation":[{"name":"From the *Department of Internal Medicine, Justus-Liebig-University, D-35392 Giessen, Germany; the \u2021Institute of Physiology, Justus-Liebig-University, D-35392 Giessen, Germany; the \u00a7Department of Rheumatology of the Medical University of L\u00fcbeck, 23538 L\u00fcbeck, Germany; and the \u2016First Medical Department, University of Mainz, 55131 Mainz, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Noll","sequence":"additional","affiliation":[{"name":"From the *Department of Internal Medicine, Justus-Liebig-University, D-35392 Giessen, Germany; the \u2021Institute of Physiology, Justus-Liebig-University, D-35392 Giessen, Germany; the \u00a7Department of Rheumatology of the Medical University of L\u00fcbeck, 23538 L\u00fcbeck, Germany; and the \u2016First Medical Department, University of Mainz, 55131 Mainz, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Csernok","sequence":"additional","affiliation":[{"name":"From the *Department of Internal Medicine, Justus-Liebig-University, D-35392 Giessen, Germany; the \u2021Institute of Physiology, Justus-Liebig-University, D-35392 Giessen, Germany; the \u00a7Department of Rheumatology of the Medical University of L\u00fcbeck, 23538 L\u00fcbeck, Germany; and the \u2016First Medical Department, University of Mainz, 55131 Mainz, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang Ludwig","family":"Gross","sequence":"additional","affiliation":[{"name":"From the *Department of Internal Medicine, Justus-Liebig-University, D-35392 Giessen, Germany; the \u2021Institute of Physiology, Justus-Liebig-University, D-35392 Giessen, Germany; the \u00a7Department of Rheumatology of the Medical University of L\u00fcbeck, 23538 L\u00fcbeck, Germany; and the \u2016First Medical Department, University of Mainz, 55131 Mainz, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Werner-Johannes","family":"Mayet","sequence":"additional","affiliation":[{"name":"From the *Department of Internal Medicine, Justus-Liebig-University, D-35392 Giessen, Germany; the \u2021Institute of Physiology, Justus-Liebig-University, D-35392 Giessen, Germany; the \u00a7Department of Rheumatology of the Medical University of L\u00fcbeck, 23538 L\u00fcbeck, Germany; and the \u2016First Medical Department, University of Mainz, 55131 Mainz, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans-Michael","family":"Piper","sequence":"additional","affiliation":[{"name":"From the *Department of Internal Medicine, Justus-Liebig-University, D-35392 Giessen, Germany; the \u2021Institute of Physiology, Justus-Liebig-University, D-35392 Giessen, Germany; the \u00a7Department of Rheumatology of the Medical University of L\u00fcbeck, 23538 L\u00fcbeck, Germany; and the \u2016First Medical Department, University of Mainz, 55131 Mainz, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Werner","family":"Seeger","sequence":"additional","affiliation":[{"name":"From the *Department of Internal Medicine, Justus-Liebig-University, D-35392 Giessen, Germany; the \u2021Institute of Physiology, Justus-Liebig-University, D-35392 Giessen, Germany; the \u00a7Department of Rheumatology of the Medical University of L\u00fcbeck, 23538 L\u00fcbeck, Germany; and the \u2016First Medical Department, University of Mainz, 55131 Mainz, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Friedrich","family":"Grimminger","sequence":"additional","affiliation":[{"name":"From the *Department of Internal Medicine, Justus-Liebig-University, D-35392 Giessen, Germany; the \u2021Institute of Physiology, Justus-Liebig-University, D-35392 Giessen, Germany; the \u00a7Department of Rheumatology of the Medical University of L\u00fcbeck, 23538 L\u00fcbeck, Germany; and the \u2016First Medical Department, University of Mainz, 55131 Mainz, 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