{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T14:49:01Z","timestamp":1787496541602,"version":"build-2736575974"},"reference-count":44,"publisher":"Rockefeller University Press","issue":"1","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1998,7,1]]},"abstract":"<jats:p>Anti\u2013double-stranded DNA (dsDNA) antibodies are the serologic abnormality characteristically associated with systemic lupus erythematosus (SLE) and may play an important role in disease pathogenesis. Although the anti-dsDNA antibodies present in SLE are indicative of an antigen-driven response, the antigen has not been conclusively identified.<\/jats:p>\n                  <jats:p>By screening a phage peptide display library, we demonstrated previously that the decapeptide DWEYSVWLSN is specifically bound by the pathogenic murine IgG2b anti-dsDNA antibody R4A. To investigate the possibility that a protein antigen might trigger lupus-like autoimmunity, we immunized BALB\/c mice with DWEYSVWLSN in adjuvant. Mice developed significant titers of IgG anti-dsDNA antibodies 2\u20133 wk after the initial immunization. Immunized mice also developed antibodies against some other lupus autoantigens, and immunoglobulin deposition was present in renal glomeruli at 49 d. Although an immune response to peptide and dsDNA was evident in BALB\/c mice, there was little response in other inbred strains.<\/jats:p>\n                  <jats:p>This study demonstrates that lupus-like anti-dsDNA reactivity can be generated in nonautoimmune mice by immunization with a peptide antigen. Peptide-induced autoimmunity may prove useful in understanding the spreading of antigenic specificities targeted in SLE. However, most importantly, the demonstration that a peptide antigen can initiate a SLE-like immune response opens a new chapter on the potential antigenic stimuli that might trigger SLE.<\/jats:p>","DOI":"10.1084\/jem.188.1.29","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T12:49:30Z","timestamp":1027687770000},"page":"29-38","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":155,"title":["Immunization with a Peptide Surrogate for Double-stranded DNA (dsDNA) Induces Autoantibody Production and Renal Immunoglobulin Deposition"],"prefix":"10.1084","volume":"188","author":[{"given":"Chaim","family":"Putterman","sequence":"first","affiliation":[{"name":"From the *Division of Rheumatology, Department of Medicine, and the \u2021Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Betty","family":"Diamond","sequence":"additional","affiliation":[{"name":"From the *Division of Rheumatology, Department of Medicine, and the \u2021Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461"},{"name":"From the *Division of Rheumatology, Department of Medicine, and the \u2021Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1998,7,1]]},"reference":[{"key":"2023072508185180600_B1","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1146\/annurev.iy.10.040192.003503","article-title":"The role of somatic mutation in the pathogenic anti-DNA response","volume":"10","author":"Diamond","year":"1992","journal-title":"Annu Rev Immunol"},{"key":"2023072508185180600_B2","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1111\/j.1600-065X.1992.tb00835.x","article-title":"Selection of immunoglobulin variable regions in autoimmunity to DNA","volume":"128","author":"Marion","year":"1992","journal-title":"Immunol Rev"},{"key":"2023072508185180600_B3","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1146\/annurev.iy.12.040194.002415","article-title":"Genetic and structural evidence for antigen selection of anti-DNA antibodies","volume":"12","author":"Radic","year":"1994","journal-title":"Annu Rev Immunol"},{"key":"2023072508185180600_B4","doi-asserted-by":"crossref","first-page":"872","DOI":"10.4049\/jimmunol.132.2.872","article-title":"Responsiveness of autoimmune and normal mice to nucleic acid antigens","volume":"132","author":"Madaio","year":"1984","journal-title":"J Immunol"},{"key":"2023072508185180600_B5","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1002\/art.1780330202","article-title":"A role for immunogenic DNA in the pathogenesis of systemic lupus erythematosus","volume":"33","author":"Pisetsky","year":"1990","journal-title":"Arthritis Rheum"},{"key":"2023072508185180600_B6","doi-asserted-by":"crossref","first-page":"1398","DOI":"10.1172\/JCI117793","article-title":"Induction of cross-reactive anti-dsDNA antibodies in preautoimmune NZB\/NZW mice by immunization with bacterial DNA","volume":"95","author":"Gilkeson","year":"1995","journal-title":"J Clin Invest"},{"key":"2023072508185180600_B7","doi-asserted-by":"crossref","first-page":"842","DOI":"10.1172\/JCI115088","article-title":"Antibodies elicited by pneumococcal antigens bear an anti-DNA\u2013associated idiotype","volume":"87","author":"Grayzel","year":"1991","journal-title":"J Clin Invest"},{"key":"2023072508185180600_B8","first-page":"507","article-title":"The sera of patients with Klebsiella infections contain an anti-DNA idiotype (16\/6) Id and anti-polynucleotide activity","volume":"67","author":"El-Roiey","year":"1987","journal-title":"Clin Exp Immunol"},{"key":"2023072508185180600_B9","doi-asserted-by":"crossref","first-page":"2151","DOI":"10.4049\/jimmunol.119.6.2151","article-title":"Mechanisms of induction of anti-DNA antibodies by bacterial lipopolysaccharide in mice. 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