{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T11:38:53Z","timestamp":1784547533314,"version":"3.55.0"},"reference-count":28,"publisher":"Rockefeller University Press","issue":"10","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1998,11,16]]},"abstract":"<jats:p>We analyzed the immune responses evoked by a series of overlapping peptides to better understand the molecular basis for respiratory syncytial virus (RSV) G protein\u2013induced eosinophilia in BALB\/c mice. In vitro stimulation of spleen cells from natural G protein\u2013primed mice showed dominant proliferative and cytokine (interferon [IFN]-\u03b3 and interleukin [IL]-5) responses to a peptide encompassing amino acids 184\u2013198. Mice vaccinated with peptide 184\u2013 198 conjugated to keyhole limpet hemocyanin showed significant pulmonary eosinophilia (39.5%) after challenge with live RSV. In contrast, mice immunized with a peptide (208\u2013222) conjugate associated with induction of IFN-\u03b3 secreting spleen cells did not exhibit pulmonary eosinophilia after challenge. The in vivo depletion of CD4+ cells abrogated pulmonary eosinophilia in mice vaccinated with the peptide 184\u2013198 conjugate, whereas the depletion of CD8+ cells had a negligible effect. Therefore, we have identified an association between peptide 184\u2013 198 of natural G protein and the CD4+ T cell\u2013mediated induction of pulmonary eosinophilia after live RSV challenge. Out of 43 human donors, 6 provided peripheral blood mononuclear cells that showed reactivity to G protein from RSV A2, 3 of which responded to peptide 184\u2013 198. The results have important implications for the development of a vaccine against RSV.<\/jats:p>","DOI":"10.1084\/jem.188.10.1967","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:49:30Z","timestamp":1027702170000},"page":"1967-1972","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":114,"title":["Atypical Pulmonary Eosinophilia Is Mediated by a Specific Amino Acid Sequence of the Attachment (G) Protein of Respiratory Syncytial Virus"],"prefix":"10.1084","volume":"188","author":[{"given":"Paul W.","family":"Tebbey","sequence":"first","affiliation":[{"name":"From the Department of Immunology Research, Wyeth-Lederle Vaccines and Pediatrics, West Henrietta, New York 14586-9728"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Hagen","sequence":"additional","affiliation":[{"name":"From the Department of Immunology Research, Wyeth-Lederle Vaccines and 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