{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T03:22:26Z","timestamp":1791256946886,"version":"4.1.0"},"reference-count":39,"publisher":"Rockefeller University Press","issue":"3","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1998,8,3]]},"abstract":"<jats:p>Bullous pemphigoid (BP) is an autoimmune subepidermal blistering disease characterized by deposition of autoantibodies at the basement membrane zone. In an experimental BP model in mice, the subepidermal blistering is mediated by antibodies directed against the hemidesmosomal protein BP180 (collagen XVII, BPAG2), and depends on complement activation and neutrophil infiltration. Gelatinase B is present in BP blister fluid and can cleave BP180. In this study we investigated the role of gelatinase B in the immunopathogenesis of experimental BP using mice containing targeted disruption of the gelatinase B (MMP-9, 92 kD gelatinase) gene. Gelatinase B\u2013deficient mice were resistant to the blistering effect of intracutaneous anti-mBP180 antibodies, although these mice showed deposition of autoantibodies at the basement membrane zone and neutrophil recruitment to the skin comparable to that observed in the control mice. Interleukin 8 given intradermally concomitantly with pathogenic anti-mBP180 elicited a significant neutrophil recruitment into the skin in gelatinase B\u2013deficient mice, but blistering did not occur. However, gelatinase B\u2013deficient mice reconstituted with neutrophils from normal mice developed blistering in response to anti-mBP180 antibodies. These results implicate neutrophil-derived gelatinase B in the pathogenesis of experimental BP and might lead to novel therapeutic strategies for BP.<\/jats:p>","DOI":"10.1084\/jem.188.3.475","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:49:30Z","timestamp":1027702170000},"page":"475-482","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":203,"title":["Gelatinase B\u2013deficient Mice Are Resistant to Experimental Bullous Pemphigoid"],"prefix":"10.1084","volume":"188","author":[{"given":"Zhi","family":"Liu","sequence":"first","affiliation":[{"name":"From the *Department of Dermatology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, and the Veterans Affairs Medical Center, Milwaukee, Wisconsin 53295; the \u2021Department of Medicine, Washington University School of Medicine at Barnes-Jewish Hospital, St. Louis, Missouri 63110; and the \u00a7Department of Anatomy, University of California, San Francisco, California 94143"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Michael Shipley","sequence":"additional","affiliation":[{"name":"From the *Department of Dermatology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, and the Veterans Affairs Medical Center, Milwaukee, Wisconsin 53295; the \u2021Department of Medicine, Washington University School of Medicine at Barnes-Jewish Hospital, St. Louis, Missouri 63110; and the \u00a7Department of Anatomy, University of California, San Francisco, California 94143"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thiennu H.","family":"Vu","sequence":"additional","affiliation":[{"name":"From the *Department of Dermatology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, and the Veterans Affairs Medical Center, Milwaukee, Wisconsin 53295; the \u2021Department of Medicine, Washington University School of Medicine at Barnes-Jewish Hospital, St. Louis, Missouri 63110; and the \u00a7Department of Anatomy, University of California, San Francisco, California 94143"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoye","family":"Zhou","sequence":"additional","affiliation":[{"name":"From the *Department of Dermatology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, and the Veterans Affairs Medical Center, Milwaukee, Wisconsin 53295; the \u2021Department of Medicine, Washington University School of Medicine at Barnes-Jewish Hospital, St. Louis, Missouri 63110; and the \u00a7Department of Anatomy, University of California, San Francisco, California 94143"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis A.","family":"Diaz","sequence":"additional","affiliation":[{"name":"From the *Department of Dermatology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, and the Veterans Affairs Medical Center, Milwaukee, Wisconsin 53295; the \u2021Department of Medicine, Washington University School of Medicine at Barnes-Jewish Hospital, St. Louis, Missouri 63110; and the \u00a7Department of Anatomy, University of California, San Francisco, California 94143"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zena","family":"Werb","sequence":"additional","affiliation":[{"name":"From the *Department of Dermatology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, and the Veterans Affairs Medical Center, Milwaukee, Wisconsin 53295; the \u2021Department of Medicine, Washington University School of Medicine at Barnes-Jewish Hospital, St. Louis, Missouri 63110; and the \u00a7Department of Anatomy, University of California, San Francisco, California 94143"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert M.","family":"Senior","sequence":"additional","affiliation":[{"name":"From the *Department of Dermatology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, and the Veterans Affairs Medical Center, Milwaukee, Wisconsin 53295; the \u2021Department of Medicine, Washington University School of Medicine at Barnes-Jewish Hospital, St. Louis, Missouri 63110; and the \u00a7Department of Anatomy, University of California, San Francisco, California 94143"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1998,8,3]]},"reference":[{"key":"2023072508160659100_B1"},{"key":"2023072508160659100_B2","doi-asserted-by":"crossref","first-page":"12555","DOI":"10.1016\/S0021-9258(18)98934-9","article-title":"Comparison of molecularly cloned bullous pemphigoid antigen to desmoplakin I confirms that they define a new family of cell adhesion junction plaque proteins","volume":"266","author":"Tanaka","year":"1991","journal-title":"J Biol Chem"},{"key":"2023072508160659100_B3","doi-asserted-by":"crossref","first-page":"17784","DOI":"10.1016\/S0021-9258(18)55195-4","article-title":"Human bullous pemphigoid antigen (BPAG1). 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