{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:54:46Z","timestamp":1791420886376,"version":"4.3.3"},"reference-count":32,"publisher":"Rockefeller University Press","issue":"2","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1999,1,18]]},"abstract":"<jats:p>Human minor histocompatibility antigens (mHags) play an important role in the induction of cytotoxic T lymphocyte (CTL) reactivity against leukemia after human histocompatibility leukocyte antigen (HLA)-identical allogeneic bone marrow transplantation (BMT). As most mHags are not leukemia specific but are also expressed by normal tissues, antileukemia reactivity is often associated with life-threatening graft-versus-host disease (GVHD). Here, we describe a novel mHag, HB-1, that elicits donor-derived CTL reactivity in a B cell acute lymphoblastic leukemia (B-ALL) patient treated by HLA-matched BMT. We identified the gene encoding the antigenic peptide recognized by HB-1\u2013specific CTLs. Interestingly, expression of the HB-1 gene was only observed in B-ALL cells and Epstein-Barr virus\u2013transformed B cells. The HB-1 gene\u2013encoded peptide EEKRGSLHVW is recognized by the CTL in association with HLA-B44. Further analysis reveals that a polymorphism in the HB-1 gene generates a single amino acid exchange from His to Tyr at position 8 within this peptide. This amino acid substitution is critical for recognition by HB-1\u2013specific CTLs. The restricted expression of the polymorphic HB-1 Ag by B-ALL cells and the ability to generate HB-1\u2013specific CTLs in vitro using peptide-loaded dendritic cells offer novel opportunities to specifically target the immune system against B-ALL without the risk of evoking GVHD.<\/jats:p>","DOI":"10.1084\/jem.189.2.301","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:49:30Z","timestamp":1027702170000},"page":"301-308","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":189,"title":["A Human Minor Histocompatibility Antigen Specific for B Cell Acute Lymphoblastic Leukemia"],"prefix":"10.1084","volume":"189","author":[{"given":"Harry","family":"Dolstra","sequence":"first","affiliation":[{"name":"From the *Department of Hematology and the \u2021Department of \u2009Tumor Immunology, University Hospital Nijmegen, 6500 HB Nijmegen, The Netherlands; and the \u00a7Cellular Genetics Unit and the \u2016Ludwig Institute for Cancer Research, Universit\u00e9 Catholique de Louvain, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanny","family":"Fredrix","sequence":"additional","affiliation":[{"name":"From the *Department of Hematology and the \u2021Department of \u2009Tumor Immunology, University Hospital Nijmegen, 6500 HB Nijmegen, The Netherlands; and the \u00a7Cellular Genetics Unit and the \u2016Ludwig Institute for Cancer Research, Universit\u00e9 Catholique de Louvain, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frans","family":"Maas","sequence":"additional","affiliation":[{"name":"From the *Department of Hematology and the \u2021Department of \u2009Tumor Immunology, University Hospital Nijmegen, 6500 HB Nijmegen, The Netherlands; and the \u00a7Cellular Genetics Unit and the \u2016Ludwig Institute for Cancer Research, Universit\u00e9 Catholique de Louvain, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre G.","family":"Coulie","sequence":"additional","affiliation":[{"name":"From the *Department of Hematology and the \u2021Department of \u2009Tumor Immunology, University Hospital Nijmegen, 6500 HB Nijmegen, The Netherlands; and the \u00a7Cellular Genetics Unit and the \u2016Ludwig Institute for Cancer Research, Universit\u00e9 Catholique de Louvain, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francis","family":"Brasseur","sequence":"additional","affiliation":[{"name":"From the *Department of Hematology and the \u2021Department of \u2009Tumor Immunology, University Hospital Nijmegen, 6500 HB Nijmegen, The Netherlands; and the \u00a7Cellular Genetics Unit and the \u2016Ludwig Institute for Cancer Research, Universit\u00e9 Catholique de Louvain, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ewald","family":"Mensink","sequence":"additional","affiliation":[{"name":"From the *Department of Hematology and the \u2021Department of \u2009Tumor Immunology, University Hospital Nijmegen, 6500 HB Nijmegen, The Netherlands; and the \u00a7Cellular Genetics Unit and the \u2016Ludwig Institute for Cancer Research, Universit\u00e9 Catholique de Louvain, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gosse J.","family":"Adema","sequence":"additional","affiliation":[{"name":"From the *Department of Hematology and the \u2021Department of \u2009Tumor Immunology, University Hospital Nijmegen, 6500 HB Nijmegen, The Netherlands; and the \u00a7Cellular Genetics Unit and the \u2016Ludwig Institute for Cancer Research, Universit\u00e9 Catholique de Louvain, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theo M.","family":"de Witte","sequence":"additional","affiliation":[{"name":"From the *Department of Hematology and the \u2021Department of \u2009Tumor Immunology, University Hospital Nijmegen, 6500 HB Nijmegen, The Netherlands; and the \u00a7Cellular Genetics Unit and the \u2016Ludwig Institute for Cancer Research, Universit\u00e9 Catholique de Louvain, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carl G.","family":"Figdor","sequence":"additional","affiliation":[{"name":"From the *Department of Hematology and the \u2021Department of \u2009Tumor Immunology, University Hospital Nijmegen, 6500 HB Nijmegen, The Netherlands; and the \u00a7Cellular Genetics Unit and the \u2016Ludwig Institute for Cancer Research, Universit\u00e9 Catholique de Louvain, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elly","family":"van de Wiel-van Kemenade","sequence":"additional","affiliation":[{"name":"From the *Department of Hematology and the \u2021Department of \u2009Tumor Immunology, University Hospital Nijmegen, 6500 HB Nijmegen, The Netherlands; and the \u00a7Cellular Genetics Unit and the \u2016Ludwig Institute for Cancer Research, Universit\u00e9 Catholique de Louvain, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1999,1,18]]},"reference":[{"key":"2023072508320225900_B1","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1182\/blood.V75.3.555.555","article-title":"Graft-versus-leukemia reactions after bone marrow transplantation","volume":"75","author":"Horowitz","year":"1990","journal-title":"Blood"},{"key":"2023072508320225900_B2","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/S0952-7915(96)80108-7","article-title":"Human minor histocompatibility antigens","volume":"8","author":"Goulmy","year":"1996","journal-title":"Curr Opin Immunol"},{"key":"2023072508320225900_B3","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/S0167-5699(97)01185-7","article-title":"Immunodominant minor histocompatibility antigens: the major ones","volume":"19","author":"Perrault","year":"1998","journal-title":"Immunol Today"},{"key":"2023072508320225900_B4","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1038\/nm0897-833","article-title":"Taming the sinister side of BMT: Dr. Jekyll and Mr. Hyde","volume":"3","author":"Pardoll","year":"1997","journal-title":"Nature Med"},{"key":"2023072508320225900_B5","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1038\/343275a0","article-title":"Identification of classical minor histocompatibility antigen as cell-derived peptide","volume":"343","author":"Wallny","year":"1990","journal-title":"Nature"},{"key":"2023072508320225900_B6","doi-asserted-by":"crossref","first-page":"1588","DOI":"10.1126\/science.7667640","article-title":"Human H-Y: a male-specific histocompatibility antigen derived from the SMCY protein","volume":"269","author":"Wang","year":"1995","journal-title":"Science"},{"key":"2023072508320225900_B7","doi-asserted-by":"crossref","first-page":"1054","DOI":"10.1126\/science.279.5353.1054","article-title":"The minor histocompatibility antigen HA-1: a diallelic gene with a single amino acid polymorphism","volume":"279","author":"Den Haan, J.M.M., L.M. 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