{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T07:01:24Z","timestamp":1792738884462,"version":"4.5.0"},"reference-count":35,"publisher":"Rockefeller University Press","issue":"4","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1999,2,15]]},"abstract":"<jats:p>N-formylpeptides derive from bacterial and mitochondrial proteins, and bind to specific receptors on mammalian phagocytes. Since binding induces chemotaxis and activation of phagocytes in vitro, it has been postulated that N-formylpeptide receptor signaling in vivo may be important in antimicrobial host defense, although direct proof has been lacking. Here we test this hypothesis in mice lacking the high affinity N-formylpeptide receptor (FPR), created by targeted gene disruption. FPR\u2212\/\u2212 mice developed normally, but had increased susceptibility to challenge with Listeria monocytogenes, as measured by increased mortality compared with wild-type littermates. FPR\u2212\/\u2212 mice also had increased bacterial load in spleen and liver 2 d after infection, which is before development of a specific cellular immune response, suggesting a defect in innate immunity. Consistent with this, neutrophil chemotaxis in vitro and neutrophil mobilization into peripheral blood in vivo in response to the prototype N-formylpeptide fMLF (formyl-methionyl-leucyl-phenylalanine) were both absent in FPR\u2212\/\u2212 mice. These results indicate that FPR functions in antibacterial host defense in vivo.<\/jats:p>","DOI":"10.1084\/jem.189.4.657","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:49:30Z","timestamp":1027702170000},"page":"657-662","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":214,"title":["Impaired Antibacterial Host Defense in Mice Lacking the <i>N<\/i>-formylpeptide Receptor"],"prefix":"10.1084","volume":"189","author":[{"given":"Ji-Liang","family":"Gao","sequence":"first","affiliation":[{"name":"From the *Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, and the \u2021Laboratory of Mammalian Genes and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric J.","family":"Lee","sequence":"additional","affiliation":[{"name":"From the *Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, and the \u2021Laboratory of Mammalian Genes and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip M.","family":"Murphy","sequence":"additional","affiliation":[{"name":"From the *Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, and the \u2021Laboratory of Mammalian Genes and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1999,2,15]]},"reference":[{"key":"2023072508305178700_B1"},{"key":"2023072508305178700_B2","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1073\/pnas.72.3.1059","article-title":"N-formylmethionyl peptides as chemoattractants for leukocytes","volume":"72","author":"Schiffmann","year":"1975","journal-title":"Proc Natl Acad Sci USA"},{"key":"2023072508305178700_B3","doi-asserted-by":"crossref","first-page":"1831","DOI":"10.4049\/jimmunol.114.6.1831","article-title":"The isolation and partial characterization of neutrophil chemotactic factors from Escherichia coli.","volume":"114","author":"Schiffmann","year":"1975","journal-title":"J Immunol"},{"key":"2023072508305178700_B4","doi-asserted-by":"crossref","first-page":"5430","DOI":"10.1016\/S0021-9258(18)91029-X","article-title":"Purification and identification of formyl-methionyl-leucyl-phenylalanine as the major peptide neutrophil chemotactic factor produced by Escherichia coli.","volume":"259","author":"Marasco","year":"1984","journal-title":"J Biol Chem"},{"key":"2023072508305178700_B5","doi-asserted-by":"crossref","first-page":"1103","DOI":"10.1016\/0006-291X(90)91143-G","article-title":"Synthesis and use of a novel N-formyl peptide derivative to isolate a human N-formyl peptide receptor cDNA","volume":"168","author":"Boulay","year":"1990","journal-title":"Biochem Biophys Res Commun"},{"key":"2023072508305178700_B6","doi-asserted-by":"crossref","first-page":"12560","DOI":"10.1016\/S0021-9258(18)98935-0","article-title":"Functional expression of the human formyl peptide receptor in Xenopusoocytes requires a complementary human factor","volume":"266","author":"Murphy","year":"1991","journal-title":"J Biol Chem"},{"key":"2023072508305178700_B7","doi-asserted-by":"crossref","first-page":"7637","DOI":"10.1016\/S0021-9258(18)42563-X","article-title":"A structural homologue of the N-formyl peptide receptor. 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