{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T06:20:00Z","timestamp":1792736400004,"version":"4.5.0"},"reference-count":42,"publisher":"Rockefeller University Press","issue":"4","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1999,2,15]]},"abstract":"<jats:p>T lymphocyte recognition of infected cells is mediated by T cell receptors (TCRs) interacting with their ligands, self\u2013major histocompatibility complex (MHC) molecules complexed with pathogen-derived peptides. Serial TCR interactions with potentially small numbers of MHC\/ peptide complexes on infected cells transmit signals that result in T lymphocyte expansion and activation of effector functions. The impact of TCR affinity for MHC\/peptide complexes on the rate or extent of in vivo T cell expansion is not known. Here we show that in vivo expansion of complex T cell populations after bacterial infection is accompanied by an increase in their overall affinity for antigen. T cell populations that have undergone additional rounds of in vivo expansion express a narrower range of TCRs, have increased sensitivity for antigen in cytotoxic T lymphocyte assays, and bind MHC\/peptide complexes with greater affinity. The selective expansion of higher affinity T cells provides an in vivo mechanism for optimizing the early detection of infected cells.<\/jats:p>","DOI":"10.1084\/jem.189.4.701","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:49:30Z","timestamp":1027702170000},"page":"701-710","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":316,"title":["T Cell Affinity Maturation by Selective Expansion during Infection"],"prefix":"10.1084","volume":"189","author":[{"given":"Dirk H.","family":"Busch","sequence":"first","affiliation":[{"name":"From the Sections of Infectious Diseases and Immunobiology, \u2009Yale University School of Medicine, New Haven, Connecticut 06511"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric G.","family":"Pamer","sequence":"additional","affiliation":[{"name":"From the Sections of Infectious Diseases and Immunobiology, \u2009Yale University School of 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