{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T05:22:39Z","timestamp":1791264159791,"version":"4.1.0"},"reference-count":90,"publisher":"Rockefeller University Press","issue":"8","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1999,4,19]]},"abstract":"<jats:p>Signal transducer and activator of transcription (STAT)5 is constitutively activated in BCR\/ ABL-expressing cells, but the mechanisms and functional consequences of such activation are unknown. We show here that BCR\/ABL induces phosphorylation and activation of STAT5 by a mechanism that requires the BCR\/ABL Src homology (SH)2 domain and the proline-rich binding site of the SH3 domain. Upon expression in 32Dcl3 growth factor\u2013dependent myeloid precursor cells, STAT5 activation\u2013deficient BCR\/ABL SH3+SH2 domain mutants functioned as tyrosine kinase and activated Ras, but failed to protect from apoptosis induced by withdrawal of interleukin 3 and\/or serum and did not induce leukemia in severe combined immunodeficiency mice. In complementation assays, expression of a dominant-active STAT5B mutant (STAT5B-DAM), but not wild-type STAT5B (STAT5B-WT), in 32Dcl3 cells transfected with STAT5 activation\u2013deficient BCR\/ABL SH3+SH2 mutants restored protection from apoptosis, stimulated growth factor\u2013independent cell cycle progression, and rescued the leukemogenic potential in mice. Moreover, expression of a dominant-negative STAT5B mutant (STAT5B-DNM) in 32Dcl3 cells transfected with wild-type BCR\/ABL inhibited apoptosis resistance, growth factor\u2013independent proliferation, and the leukemogenic potential of these cells. In retrovirally infected mouse bone marrow cells, expression of STAT5B-DNM inhibited BCR\/ABL-dependent transformation. Moreover, STAT5B-DAM, but not STAT5B-WT, markedly enhanced the ability of STAT5 activation\u2013defective BCR\/ABL SH3+SH2 mutants to induce growth factor\u2013independent colony formation of primary mouse bone marrow progenitor cells. However, STAT5B-DAM did not rescue the growth factor\u2013independent colony formation of kinase-deficient K1172R BCR\/ABL or the triple mutant Y177F+R522L+ Y793F BCR\/ABL, both of which also fail to activate STAT5. Together, these data demonstrate that STAT5 activation by BCR\/ABL is dependent on signaling from more than one domain and document the important role of STAT5-regulated pathways in BCR\/ABL leukemogenesis.<\/jats:p>","DOI":"10.1084\/jem.189.8.1229","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T12:48:33Z","timestamp":1027687713000},"page":"1229-1242","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":234,"title":["Signal Transducer and Activator of \u2009Transcription (STAT)5 Activation by BCR\/ABL Is Dependent on Intact Src Homology (SH)3 and SH2 Domains of BCR\/ABL and Is Required for Leukemogenesis"],"prefix":"10.1084","volume":"189","author":[{"given":"Malgorzata","family":"Nieborowska-Skorska","sequence":"first","affiliation":[{"name":"From the *Department of Microbiology and Immunology, Kimmel Cancer Center, \u2009Thomas Jefferson University, Philadelphia, Pennsylvania 19107; the \u2021Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104; and the \u00a7Department of Hematopoietic Factors, Institute of Medical Science, University of \u2009Tokyo, Tokyo 108, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariusz A.","family":"Wasik","sequence":"additional","affiliation":[{"name":"From the *Department of Microbiology and Immunology, Kimmel Cancer Center, \u2009Thomas Jefferson University, Philadelphia, Pennsylvania 19107; the \u2021Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104; and the \u00a7Department of Hematopoietic Factors, Institute of Medical Science, University of \u2009Tokyo, Tokyo 108, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Artur","family":"Slupianek","sequence":"additional","affiliation":[{"name":"From the *Department of Microbiology and Immunology, Kimmel Cancer Center, \u2009Thomas Jefferson University, Philadelphia, Pennsylvania 19107; the \u2021Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104; and the \u00a7Department of Hematopoietic Factors, Institute of Medical Science, University of \u2009Tokyo, Tokyo 108, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Salomoni","sequence":"additional","affiliation":[{"name":"From the *Department of Microbiology and Immunology, Kimmel Cancer Center, \u2009Thomas Jefferson University, Philadelphia, Pennsylvania 19107; the \u2021Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104; and the \u00a7Department of Hematopoietic Factors, Institute of Medical Science, University of \u2009Tokyo, Tokyo 108, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshio","family":"Kitamura","sequence":"additional","affiliation":[{"name":"From the *Department of Microbiology and Immunology, Kimmel Cancer Center, \u2009Thomas Jefferson University, Philadelphia, Pennsylvania 19107; the \u2021Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104; and the \u00a7Department of Hematopoietic Factors, Institute of Medical Science, University of \u2009Tokyo, Tokyo 108, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Calabretta","sequence":"additional","affiliation":[{"name":"From the *Department of Microbiology and Immunology, Kimmel Cancer Center, \u2009Thomas Jefferson University, Philadelphia, Pennsylvania 19107; the \u2021Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104; and the \u00a7Department of Hematopoietic Factors, Institute of Medical Science, University of \u2009Tokyo, Tokyo 108, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomasz","family":"Skorski","sequence":"additional","affiliation":[{"name":"From the *Department of Microbiology and Immunology, Kimmel Cancer Center, \u2009Thomas Jefferson University, Philadelphia, Pennsylvania 19107; the \u2021Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104; and the \u00a7Department of Hematopoietic Factors, Institute of Medical Science, University of \u2009Tokyo, Tokyo 108, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1999,4,19]]},"reference":[{"key":"2023072508431474100_B1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.7326\/0003-4819-113-1-3","article-title":"Molecular genetics of advances in chronic myelogenous leukemia","volume":"113","author":"Epner","year":"1990","journal-title":"Ann Intern Med"},{"key":"2023072508431474100_B2","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/0092-8674(86)90450-2","article-title":"Alternative splicing of RNAs transcribed from the human abl gene and from the BCR\/ABL fused gene","volume":"47","author":"Shtivelman","year":"1986","journal-title":"Cell"},{"key":"2023072508431474100_B3","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1126\/science.3422516","article-title":"Expression of a distinctive bcr\/abl oncogene in Ph1-positive acute lymphoblastic leukemia (ALL)","volume":"238","author":"Clark","year":"1988","journal-title":"Science"},{"key":"2023072508431474100_B4","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1126\/science.2408149","article-title":"Tyrosine kinase activity and transformation potency of bcr-abl oncogene products","volume":"247","author":"Lugo","year":"1990","journal-title":"Science"},{"key":"2023072508431474100_B5","doi-asserted-by":"crossref","first-page":"2038","DOI":"10.1182\/blood.V83.8.2038.2038","article-title":"Inhibition of apoptosis by BCR\/ABL in chronic myelogenous leukemia","volume":"83","author":"Bedi","year":"1994","journal-title":"Blood"},{"key":"2023072508431474100_B6","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.1182\/blood.V83.5.1179.1179","article-title":"BCR\/ABL maintains resistance of chronic myelogenous leukemia cells to apoptotic cell death","volume":"83","author":"McGahon","year":"1994","journal-title":"Blood"},{"key":"2023072508431474100_B7","doi-asserted-by":"crossref","first-page":"1575","DOI":"10.1182\/blood.V83.6.1575.1575","article-title":"Expression of BCR\/ABL abrogates factor-dependent growth of human hematopoietic M07E cells by an autocrine mechanism","volume":"83","author":"Sirard","year":"1994","journal-title":"Blood"},{"key":"2023072508431474100_B8","doi-asserted-by":"crossref","first-page":"1232","DOI":"10.1172\/JCI115985","article-title":"Mechanisms underlying abnormal trafficking of malignant progenitors in chronic myelogenous leukemia","volume":"90","author":"Verfaille","year":"1992","journal-title":"J Clin Invest"},{"key":"2023072508431474100_B9","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1182\/blood.V91.2.406","article-title":"The SH3 domain contributes to BCR\/ABL-dependent leukemogenesis in vivo: role in adhesion, invasion and homing","volume":"91","author":"Skorski","year":"1998","journal-title":"Blood"},{"key":"2023072508431474100_B10","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1126\/science.2406902","article-title":"Induction of chronic myelogenous leukemia in mice by the p210bcr\/ablgene of the Philadelphia chromosome","volume":"247","author":"Daley","year":"1990","journal-title":"Science"},{"key":"2023072508431474100_B11","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1038\/344251a0","article-title":"Acute leukemia in bcr\/abl transgenic mice","volume":"344","author":"Heisterkamp","year":"1990","journal-title":"Nature"},{"key":"2023072508431474100_B12","doi-asserted-by":"crossref","first-page":"6649","DOI":"10.1073\/pnas.87.17.6649","article-title":"Induction of a chronic myelogenous leukemia-like syndrome in mice with v-abl and BCR\/ABL","volume":"87","author":"Kelleher","year":"1990","journal-title":"Proc Natl Acad Sci USA"},{"key":"2023072508431474100_B13","first-page":"F201","article-title":"Signal transduction by wild-type and leukemic Abl proteins","volume":"1333","author":"Raitano","year":"1997","journal-title":"Biochim Biophys Acta"},{"key":"2023072508431474100_B14","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/S1359-6101(96)00047-0","article-title":"Activation of hematopoietic growth factor signal transduction pathways by the human oncogene BCR\/ABL","volume":"8","author":"Sattler","year":"1997","journal-title":"Cytokine Growth Factor Rev"},{"key":"2023072508431474100_B15","doi-asserted-by":"crossref","first-page":"1855","DOI":"10.1084\/jem.179.6.1855","article-title":"Negative regulation of p120GAP GTPase promoting activity by p210bcr\/abl: implication for RAS-dependent Philadelphia chromosome positive cell growth","volume":"179","author":"Skorski","year":"1994","journal-title":"J Exp Med"},{"key":"2023072508431474100_B16","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1084\/jem.181.1.307","article-title":"Genetic requirement for Ras in the transformation of fibroblasts and hematopoietic cells by the BCR\/ABL oncogene","volume":"181","author":"Sawyers","year":"1995","journal-title":"J Exp Med"},{"key":"2023072508431474100_B17","doi-asserted-by":"crossref","first-page":"11858","DOI":"10.1073\/pnas.95.20.11858","article-title":"BCR\/ABL-mediated leukemogenesis requires the activity of the small GTP-binding protein Rac","volume":"95","author":"Skorski","year":"1998","journal-title":"Proc Natl Acad Sci USA"},{"key":"2023072508431474100_B18","first-page":"2275","article-title":"c-RAF-1 serine\/threonine kinase is required in BCR\/ABL-dependent and normal hematopoiesis","volume":"55","author":"Skorski","year":"1995","journal-title":"Cancer Res"},{"key":"2023072508431474100_B19","first-page":"1107","article-title":"Activation of phosphatidylinositol 3-kinase in cells expressing abl oncogene variants","volume":"11","author":"Varticovski","year":"1991","journal-title":"Mol Cell Biol"},{"key":"2023072508431474100_B20","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1182\/blood.V86.2.726.bloodjournal862726","article-title":"Phosphatidylinositol-3 kinase activity is regulated by BCR\/ABL and is required for the growth of Philadelphia chromosome-positive cells","volume":"86","author":"Skorski","year":"1995","journal-title":"Blood"},{"key":"2023072508431474100_B21","doi-asserted-by":"crossref","first-page":"6151","DOI":"10.1093\/emboj\/16.20.6151","article-title":"Transformation of hematopoietic cells by BCR\/ABL requires the activation of a PI-3k\/ Akt-dependent pathway","volume":"16","author":"Skorski","year":"1997","journal-title":"EMBO (Eur Mol Biol Organ) J"},{"key":"2023072508431474100_B22","doi-asserted-by":"crossref","first-page":"5287","DOI":"10.1073\/pnas.92.12.5287","article-title":"Tumorigenic activity of the BCR\/ABL oncogenes is mediated by BCL-2","volume":"92","author":"Sanchez-Garcia","year":"1995","journal-title":"Proc Natl Acad Sci USA"},{"key":"2023072508431474100_B23","doi-asserted-by":"crossref","first-page":"968","DOI":"10.1101\/gad.12.7.968","article-title":"A requirement for NF-\u03baB activation in Bcr-Abl-mediated transformation","volume":"12","author":"Reuther","year":"1998","journal-title":"Genes Dev"},{"key":"2023072508431474100_B24","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1084\/jem.183.3.811","article-title":"Tyrosine phosphorylation and DNA binding activity of signal transducers and activators of transcription (STAT) proteins in hematopoietic cell lines transformed by BCR\/ABL","volume":"183","author":"Carlesso","year":"1996","journal-title":"J Exp Med"},{"key":"2023072508431474100_B25","first-page":"1724","article-title":"BCR\/ABL leads to the constitutive activation of STAT proteins, and shares an epitope with tyrosine phosphorylated STAT5","volume":"10","author":"Frank","year":"1996","journal-title":"Leukemia"},{"key":"2023072508431474100_B26","doi-asserted-by":"crossref","first-page":"31704","DOI":"10.1074\/jbc.271.49.31704","article-title":"p210 and p190bcr\/ablinduce the tyrosine phosphorylation and DNA binding activity of multiple specific STAT family members","volume":"271","author":"Ilaria","year":"1996","journal-title":"J Biol Chem"},{"key":"2023072508431474100_B27","first-page":"247","article-title":"Constitutive activation of STAT5 by the BCR\/ ABL oncogene in chronic myelogenous leukemia","volume":"13","author":"Shuai","year":"1996","journal-title":"Oncogene"},{"key":"2023072508431474100_B28","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/S0092-8674(00)81277-5","article-title":"STATs: signal transducers and activation of transcription","volume":"84","author":"Ihle","year":"1996","journal-title":"Cell"},{"key":"2023072508431474100_B29","doi-asserted-by":"crossref","first-page":"1630","DOI":"10.1126\/science.277.5332.1630","article-title":"STATs and gene regulation","volume":"277","author":"Darnell","year":"1997","journal-title":"Science"},{"key":"2023072508431474100_B30","first-page":"2266","article-title":"STAT5A is mandatory for adult gland development and lactogenesis","volume":"9","author":"Liu","year":"1997","journal-title":"Genes Dev"},{"key":"2023072508431474100_B31","doi-asserted-by":"crossref","first-page":"7239","DOI":"10.1073\/pnas.94.14.7239","article-title":"Requirement of STAT5B for sexual dimorphism of body growth rates and liver gene expression","volume":"94","author":"Udy","year":"1997","journal-title":"Proc Natl Acad Sci USA"},{"key":"2023072508431474100_B32","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1016\/S0092-8674(00)81444-0","article-title":"Stat5a and Stat5b proteins have essential and nonessential, or redundant, roles in cytokine responses","volume":"93","author":"Teglund","year":"1998","journal-title":"Cell"},{"key":"2023072508431474100_B33","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/1074-7613(95)90141-8","article-title":"The role of shared receptor motifs and common STAT proteins in the generation of cytokine pleiotropy and redundancy by IL-2, IL-4, IL-7, IL-13, and IL-15","volume":"2","author":"Lin","year":"1995","journal-title":"Immunity"},{"key":"2023072508431474100_B34","doi-asserted-by":"crossref","first-page":"3502","DOI":"10.4049\/jimmunol.160.7.3502","article-title":"Regulation of cell growth by IL-2: role of STAT5 in protection from apoptosis but not in cell cycle progression","volume":"160","author":"Zamorano","year":"1998","journal-title":"J Immunol"},{"key":"2023072508431474100_B35","doi-asserted-by":"crossref","first-page":"1402","DOI":"10.1002\/j.1460-2075.1995.tb07126.x","article-title":"Interleukin-3 signals through multiple isoforms of STAT5","volume":"14","author":"Azam","year":"1995","journal-title":"EMBO (Eur Mol Biol Organ) J"},{"key":"2023072508431474100_B36","doi-asserted-by":"crossref","first-page":"1166","DOI":"10.1002\/j.1460-2075.1995.tb07100.x","article-title":"Interleukin-3, granulocyte-macrophage colony stimulating factor and interleukin-5 signals through two STAT5 homologs","volume":"14","author":"Mui","year":"1995","journal-title":"EMBO (Eur Mol Biol Organ) J"},{"key":"2023072508431474100_B37","doi-asserted-by":"crossref","first-page":"2527","DOI":"10.1002\/j.1460-2075.1995.tb07250.x","article-title":"Interleukin-2 and erythropoietin activate STAT5\/MGF via distinct pathways","volume":"14","author":"Wakao","year":"1995","journal-title":"EMBO (Eur Mol Biol Organ) J"},{"key":"2023072508431474100_B38","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1111\/j.1432-1033.1997.0411a.x","article-title":"Identification of STAT5b as a substrate of the insulin receptor","volume":"250","author":"Sawka-Verhelle","year":"1997","journal-title":"Eur J Biochem"},{"key":"2023072508431474100_B39","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1126\/science.8140422","article-title":"STAT3: a STAT family member activated by tyrosine phosphorylation in response to epidermal growth factor and interleukin-6","volume":"264","author":"Zhong","year":"1994","journal-title":"Science"},{"key":"2023072508431474100_B40","doi-asserted-by":"crossref","first-page":"13262","DOI":"10.1074\/jbc.270.22.13262","article-title":"Intermittent plasma growth hormone triggers tyrosine phosphorylation and nuclear translocation of a liver expressed, STAT5-related DNA binding protein","volume":"270","author":"Waxman","year":"1995","journal-title":"J Biol Chem"},{"key":"2023072508431474100_B41","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1038\/34657","article-title":"Induction of epithelial tubules by growth factor HGF depends on the STAT pathway","volume":"391","author":"Boccaccio","year":"1998","journal-title":"Nature"},{"key":"2023072508431474100_B42","doi-asserted-by":"crossref","first-page":"1875","DOI":"10.1126\/science.7569929","article-title":"Jak-STAT signalling induced by the v-abl oncogene","volume":"269","author":"Danial","year":"1995","journal-title":"Science"},{"key":"2023072508431474100_B43","doi-asserted-by":"crossref","first-page":"3295","DOI":"10.1128\/MCB.17.6.3295","article-title":"Abrogation of interleukin-3 dependence of myeloid cells by v-src oncogene requires SH2 and SH3 domains which specify activation of STAT","volume":"17","author":"Chaturvedi","year":"1997","journal-title":"Mol Cell Biol"},{"key":"2023072508431474100_B44","doi-asserted-by":"crossref","first-page":"5206","DOI":"10.4049\/jimmunol.159.11.5206","article-title":"Constitutive activation of the Janus kinase-STAT pathway in T-lymphoma overexpressing the Lck protein tyrosine kinase","volume":"159","author":"Yu","year":"1997","journal-title":"J Immunol"},{"key":"2023072508431474100_B45","doi-asserted-by":"crossref","first-page":"4341","DOI":"10.1182\/blood.V90.11.4341","article-title":"The Bmx tyrosine kinase induces activation of the STAT signalling pathway, which is specifically inhibited by protein kinase","volume":"90","author":"Saharinen","year":"1997","journal-title":"Blood"},{"key":"2023072508431474100_B46","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/0092-8674(95)90311-9","article-title":"Maximal activation of transcription of STAT1 and STAT3 requires both tyrosine and serine phosphorylation","volume":"82","author":"Wen","year":"1995","journal-title":"Cell"},{"key":"2023072508431474100_B47","doi-asserted-by":"crossref","first-page":"1990","DOI":"10.1126\/science.7701321","article-title":"Requirement of serine phosphorylation for formation of STAT-promoter complexes","volume":"267","author":"Zhang","year":"1995","journal-title":"Science"},{"key":"2023072508431474100_B48","doi-asserted-by":"crossref","first-page":"2425","DOI":"10.1002\/j.1460-2075.1996.tb00600.x","article-title":"Suppression of interleukin-3-induced gene expression by a C-terminal truncated STAT5: role of STAT5 in proliferation","volume":"15","author":"Mui","year":"1996","journal-title":"EMBO (Eur Mol Biol Organ) J"},{"key":"2023072508431474100_B49","doi-asserted-by":"crossref","first-page":"6141","DOI":"10.1128\/MCB.16.11.6141","article-title":"Naturally occurring dominant negative variants of STAT5","volume":"16","author":"Wang","year":"1996","journal-title":"Mol Cell Biol"},{"key":"2023072508431474100_B50","doi-asserted-by":"crossref","first-page":"1901","DOI":"10.1182\/blood.V91.6.1901","article-title":"Carboxyl-truncated STAT5b is generated by a nucleus-associated serine protease in early hematopoietic progenitors","volume":"91","author":"Meyer","year":"1998","journal-title":"Blood"},{"key":"2023072508431474100_B51","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1002\/stem.140605","article-title":"Function and signal transduction mediated by the interleukin-3 receptor system in hematopoiesis","volume":"14","author":"Hara","year":"1996","journal-title":"Stem Cells"},{"key":"2023072508431474100_B52","doi-asserted-by":"crossref","first-page":"1768","DOI":"10.1182\/blood.V90.5.1768","article-title":"STAT5A-deficient mice demonstrate a defect in granulocyte-macrophage colony-stimulating factor-induced proliferation and gene expression","volume":"90","author":"Feldman","year":"1997","journal-title":"Blood"},{"key":"2023072508431474100_B53","doi-asserted-by":"crossref","first-page":"1692","DOI":"10.1182\/blood.V87.5.1692.1692","article-title":"STAT- related transcription factors are constitutively activated in peripheral blood cells from acute leukemia patients","volume":"87","author":"Gouilleux-Gouart","year":"1996","journal-title":"Blood"},{"key":"2023072508431474100_B54","doi-asserted-by":"crossref","first-page":"9148","DOI":"10.1073\/pnas.93.17.9148","article-title":"Activation of Jak\/STAT proteins involved in signal transduction pathway mediated by receptor for interleukin-2 in malignant T lymphocytes derived from cutaneous anaplastic large T-cell lymphoma and Sezary syndrome","volume":"93","author":"Zhang","year":"1996","journal-title":"Proc Natl Acad Sci USA"},{"key":"2023072508431474100_B55","doi-asserted-by":"crossref","first-page":"4720","DOI":"10.4049\/jimmunol.159.10.4720","article-title":"Constitutive activation of JAKs and STATs in BCR\/ABL-expressing cell lines and peripheral blood cells derived from leukemic patients","volume":"159","author":"Chai","year":"1997","journal-title":"J Immunol"},{"key":"2023072508431474100_B56","doi-asserted-by":"crossref","first-page":"5531","DOI":"10.1128\/MCB.15.10.5531","article-title":"Structural and signalling requirements for BCR\/ABL-mediated transformation and inhibition of apoptosis","volume":"15","author":"Cortez","year":"1995","journal-title":"Mol Cell Biol"},{"key":"2023072508431474100_B57","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/0092-8674(91)90148-R","article-title":"BCR sequences essential for transformation by the BCR\/ABL oncogene bind to the ABL SH2 regulatory domain in a non-phosphotyrosine-dependent manner","volume":"66","author":"Pendergast","year":"1991","journal-title":"Cell"},{"key":"2023072508431474100_B58","doi-asserted-by":"crossref","first-page":"3871","DOI":"10.1128\/MCB.18.7.3871","article-title":"Identification and characterization of a constitutively active STAT5 mutant that promotes cell proliferation","volume":"18","author":"Onishi","year":"1998","journal-title":"Mol Cell Biol"},{"key":"2023072508431474100_B59","doi-asserted-by":"crossref","first-page":"2931","DOI":"10.1073\/pnas.80.10.2931","article-title":"Demonstration of permanent factor-dependent multipotential (erythroid\/neutrophil\/eosinophil) hematopoietic progenitor cell lines","volume":"80","author":"Greenberger","year":"1983","journal-title":"Proc Natl Acad Sci USA"},{"key":"2023072508431474100_B60","doi-asserted-by":"crossref","first-page":"13137","DOI":"10.1073\/pnas.93.23.13137","article-title":"Blastic transformation of p53-deficient bone marrow cells by p210bcr\/abltyrosine kinase","volume":"93","author":"Skorski","year":"1996","journal-title":"Proc Natl Acad Sci USA"},{"key":"2023072508431474100_B61","doi-asserted-by":"crossref","first-page":"8392","DOI":"10.1073\/pnas.90.18.8392","article-title":"Production of high-titer helper-free retroviruses by transient transfection","volume":"90","author":"Pear","year":"1993","journal-title":"Proc Natl Acad Sci USA"},{"key":"2023072508431474100_B62","first-page":"204","article-title":"Activation of the c-abl oncogene by viral transduction or chromosomal translocation generates altered c-abl proteins with similar in vitro kinase properties","volume":"5","author":"Davis","year":"1985","journal-title":"Mol Cell Biol"},{"key":"2023072508431474100_B63","doi-asserted-by":"crossref","first-page":"1645","DOI":"10.1084\/jem.182.6.1645","article-title":"Leukemia treatment in severe combined immunodeficient mice by antisense oligodeoxynucleotides targeting cooperating oncogenes","volume":"182","author":"Skorski","year":"1995","journal-title":"J Exp Med"},{"key":"2023072508431474100_B64","first-page":"1977","article-title":"Introduction of a loss-of-function point mutation from the SH3 region of the Caenorhabditis eleganssem-5 gene activates the transforming ability of c-abl in vivo and abolishes binding of proline-rich ligands in vitro","volume":"10","author":"Van Etten","year":"1995","journal-title":"Oncogene"},{"key":"2023072508431474100_B65","first-page":"609","article-title":"Point mutations in the ABL SH2 domain coordinately impair phosphotyrosine binding in vitro and transforming activity in vivo","volume":"12","author":"Mayer","year":"1992","journal-title":"Mol Cell Biol"},{"key":"2023072508431474100_B66","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1016\/S0969-2126(96)00116-5","article-title":"Intramolecular interactions of the regulatory domains of the BCR\/ABL kinase reveal a novel control mechanism","volume":"4","author":"Ham","year":"1996","journal-title":"Structure"},{"key":"2023072508431474100_B67","doi-asserted-by":"crossref","first-page":"1318","DOI":"10.1128\/MCB.15.3.1318","article-title":"A human protein selected for interference with Ras function interacts directly with Ras and competes with Raf 1","volume":"15","author":"Han","year":"1995","journal-title":"Mol Cell Biol"},{"key":"2023072508431474100_B68","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1016\/S1074-7613(00)80452-5","article-title":"Regulation of the oncogenic activity of BCR-ABL by a tightly bound substrate protein RIN1","volume":"6","author":"Afar","year":"1997","journal-title":"Immunity"},{"key":"2023072508431474100_B69","doi-asserted-by":"crossref","first-page":"6795","DOI":"10.1128\/MCB.18.11.6795","article-title":"Direct interaction of Jak 1 and v-Abl is required for v-Abl- induced activation of STATs and proliferation","volume":"18","author":"Danial","year":"1998","journal-title":"Mol Cell Biol"},{"key":"2023072508431474100_B70","doi-asserted-by":"crossref","first-page":"1383","DOI":"10.1038\/sj.onc.1201664","article-title":"Bcl-2-independent Bcr-Abl-mediated resistance to apoptosis: protection is correlated with up regulation of Bcl-XL","volume":"16","author":"Amarante-Mendes","year":"1998","journal-title":"Oncogene"},{"key":"2023072508431474100_B71","first-page":"2589","article-title":"The BCR\/ABL tyrosine kinase inhibits apoptosis by activating a RAS-dependent signaling pathway","volume":"13","author":"Cortez","year":"1996","journal-title":"Oncogene"},{"key":"2023072508431474100_B72","doi-asserted-by":"crossref","first-page":"1995","DOI":"10.1084\/jem.187.12.1995","article-title":"Expression of constitutively active Raf-1 in the mitochondria restores anti-apoptotic and leukemogenic potential of a transformation-deficient BCR\/ABL mutant","volume":"187","author":"Salomoni","year":"1998","journal-title":"J Exp Med"},{"key":"2023072508431474100_B73","doi-asserted-by":"crossref","first-page":"33936","DOI":"10.1074\/jbc.273.51.33936","article-title":"A single amino acid in the DNA binding regions of STAT5A and STAT5B confers distinct DNA binding specificities","volume":"273","author":"Boucheron","year":"1998","journal-title":"J Biol Chem"},{"key":"2023072508431474100_B74","doi-asserted-by":"crossref","first-page":"1979","DOI":"10.4049\/jimmunol.151.4.1979","article-title":"Characterization of A1, a novel hematopoietic-specific early-response gene with sequence similarity to bcl-2","volume":"151","author":"Lin","year":"1993","journal-title":"J Immunol"},{"key":"2023072508431474100_B75","doi-asserted-by":"crossref","first-page":"8983","DOI":"10.1074\/jbc.272.14.8983","article-title":"The Src homology 2 (SH2) domain of SH2-containing inositol phosphatase (SHIP) is essential for tyrosine phosphorylation of SHIP, its association with Shc, and its induction of apoptosis","volume":"272","author":"Liu","year":"1997","journal-title":"J Biol Chem"},{"key":"2023072508431474100_B76","doi-asserted-by":"crossref","first-page":"2379","DOI":"10.1038\/sj.onc.1201422","article-title":"The phosphatidylinositol polyphosphate 5-phosphatase SHIP and the protein tyrosine phosphatase SHP-2 form a complex in hematopoietic cells which can be regulated by BCR\/ABL and growth factors","volume":"15","author":"Sattler","year":"1997","journal-title":"Oncogene"},{"key":"2023072508431474100_B77","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1006\/bbrc.1998.9445","article-title":"Association of CrkL with STAT5 in hematopoietic cells stimulated by granulocyte-macrophage colony-stimulating factor or erythroprotein","volume":"252","author":"Ota","year":"1998","journal-title":"Biochem Biophys Res Commun"},{"key":"2023072508431474100_B78","doi-asserted-by":"crossref","first-page":"3148","DOI":"10.1182\/blood.V89.9.3148","article-title":"CIS, a cytokine inducible SH2 protein is a target of the JAK-STAT5 pathway and modulates STAT5 activation","volume":"89","author":"Matsumoto","year":"1997","journal-title":"Blood"},{"key":"2023072508431474100_B79","doi-asserted-by":"crossref","first-page":"2333","DOI":"10.1038\/sj.onc.1201400","article-title":"The BCR-ABL tyrosine kinase activates mitogenic signalling pathways and stimulates G1-to-S phase transition in hematopoietic cells","volume":"15","author":"Cortez","year":"1997","journal-title":"Oncogene"},{"key":"2023072508431474100_B80","doi-asserted-by":"crossref","first-page":"5887","DOI":"10.1002\/j.1460-2075.1994.tb06934.x","article-title":"A temperature sensitive p210 BCR-ABL mutant defines the primary consequences of BCR-ABL tyrosine kinase expression in growth factor dependent cells","volume":"13","author":"Kabarowski","year":"1994","journal-title":"EMBO (Eur Mol Biol Organ) J"},{"key":"2023072508431474100_B81","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1016\/0092-8674(95)90277-5","article-title":"Alternative signals to RAS for hematopoietic transformation by the BCR-ABL oncogene","volume":"82","author":"Goga","year":"1995","journal-title":"Cell"},{"key":"2023072508431474100_B82","doi-asserted-by":"crossref","first-page":"1575","DOI":"10.1182\/blood.V83.6.1575.1575","article-title":"Expression of bcr-abl abrogate factor-dependent growth of human hematopoietic MO7E cells by an autocrine mechanisms","volume":"83","author":"Sirard","year":"1994","journal-title":"Blood"},{"key":"2023072508431474100_B83","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1182\/blood.V87.1.238.238","article-title":"The BCR-ABL oncogene requires both kinase activity and src-homology 2 domain to induce cytokine secretion","volume":"87","author":"Anderson","year":"1996","journal-title":"Blood"},{"key":"2023072508431474100_B84","doi-asserted-by":"crossref","first-page":"3927","DOI":"10.1182\/blood.V91.10.3927.3927_3927_3934","article-title":"Secondary mutation maintains the transformed state in BaF3 cells with inducible BCR\/ABL expression","volume":"91","author":"Klucher","year":"1998","journal-title":"Blood"},{"key":"2023072508431474100_B85","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1182\/blood.V87.3.983.bloodjournal873983","article-title":"A1, a Bcl-2 family member, prolongs cell survival and permits myeloid differentiation","volume":"87","author":"Lin","year":"1996","journal-title":"Blood"},{"key":"2023072508431474100_B86","first-page":"3879","article-title":"Bfl-1, a bcl-2 homologue, suppresses p53 induced apoptosis and exhibits potent cooperative-transforming activity","volume":"56","author":"D'Sa-Eipper","year":"1996","journal-title":"Cancer Res"},{"key":"2023072508431474100_B87","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1016\/0092-8674(89)90589-8","article-title":"Predisposition to lymphomagenesis in pim-1 transgenic mice: cooperation with c-myc and N-myc in murine leukemia virus- induced tumors","volume":"56","author":"van Lohuizen","year":"1989","journal-title":"Cell"},{"key":"2023072508431474100_B88","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/S1097-2765(00)80141-0","article-title":"Pim-1 kinase and p100 cooperate to enhance c-Myb activity","volume":"2","author":"Leverson","year":"1998","journal-title":"Mol Cell"},{"key":"2023072508431474100_B89","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1016\/0092-8674(92)90241-4","article-title":"Dominant negative MYC blocks transformation by ABL oncogenes","volume":"70","author":"Sawyers","year":"1992","journal-title":"Cell"},{"key":"2023072508431474100_B90","doi-asserted-by":"crossref","first-page":"2351","DOI":"10.1073\/pnas.88.6.2351","article-title":"Normal and leukemic hematopoietic cells manifest differential sensitivity to inhibitory effects of c-myb antisense oligodeoxynucleotides: an in vitro study relevant to bone marrow purging","volume":"88","author":"Calabretta","year":"1991","journal-title":"Proc Natl Acad Sci USA"}],"container-title":["The Journal of Experimental Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/189\/8\/1229\/1694079\/98-1836.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jem\/article-pdf\/189\/8\/1229\/1694079\/98-1836.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T05:54:39Z","timestamp":1690264479000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jem\/article\/189\/8\/1229\/25634\/Signal-Transducer-and-Activator-of-Transcription"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,4,19]]},"references-count":90,"journal-issue":{"issue":"8","published-print":{"date-parts":[[1999,4,19]]}},"URL":"https:\/\/doi.org\/10.1084\/jem.189.8.1229","relation":{},"ISSN":["0022-1007","1540-9538"],"issn-type":[{"value":"0022-1007","type":"print"},{"value":"1540-9538","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,4,19]]}}}