{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:22:20Z","timestamp":1791354140025,"version":"4.3.1"},"reference-count":37,"publisher":"Rockefeller University Press","issue":"2","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1999,7,19]]},"abstract":"<jats:p>We demonstrated previously that a single injection of recombinant human macrophage colony-stimulating factor (rhM-CSF) is sufficient for osteoclast recruitment and survival in osteopetrotic (op\/op) mice with a deficiency in osteoclasts resulting from a mutation in M-CSF gene. In this study, we show that a single injection of recombinant human vascular endothelial growth factor (rhVEGF) can similarly induce osteoclast recruitment in op\/op mice. Osteoclasts predominantly expressed VEGF receptor 1 (VEGFR-1), and activity of recombinant human placenta growth factor 1 on osteoclast recruitment was comparable to that of rhVEGF, showing that the VEGF signal is mediated through VEGFR-1. The rhM-CSF\u2013induced osteoclasts died after injections of VEGFR-1\/Fc chimeric protein, and its effect was abrogated by concomitant injections of rhM-CSF. Osteoclasts supported by rhM-CSF or endogenous VEGF showed no significant difference in the bone-resorbing activity. op\/op mice undergo an age-related resolution of osteopetrosis accompanied by an increase in osteoclast number. Most of the osteoclasts disappeared after injections of anti-VEGF antibody, demonstrating that endogenously produced VEGF is responsible for the appearance of osteoclasts in the mutant mice. In addition, rhVEGF replaced rhM-CSF in the support of in vitro osteoclast differentiation. These results demonstrate that M-CSF and VEGF have overlapping functions in the support of osteoclastic bone resorption.<\/jats:p>","DOI":"10.1084\/jem.190.2.293","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:48:33Z","timestamp":1027702113000},"page":"293-298","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":356,"title":["Vascular Endothelial Growth Factor Can Substitute for Macrophage Colony-Stimulating Factor in the Support of Osteoclastic Bone Resorption"],"prefix":"10.1084","volume":"190","author":[{"given":"Shumpei","family":"Niida","sequence":"first","affiliation":[{"name":"aFrom the Department of Anatomy, Hiroshima University School of Dentistry, Hiroshima 734-8553, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masato","family":"Kaku","sequence":"additional","affiliation":[{"name":"bFrom the Department of Orthodontics, Hiroshima University School of Dentistry, Hiroshima 734-8553, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hitoshi","family":"Amano","sequence":"additional","affiliation":[{"name":"cDepartment of Pharmacology, School of Dentistry, Showa University, Tokyo 142-8555, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hisahiro","family":"Yoshida","sequence":"additional","affiliation":[{"name":"dDepartment of Molecular Genetics, Faculty of Medicine, Kyoto University, Kyoto 606-8507, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Kataoka","sequence":"additional","affiliation":[{"name":"dDepartment of Molecular Genetics, Faculty of Medicine, Kyoto University, Kyoto 606-8507, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satomi","family":"Nishikawa","sequence":"additional","affiliation":[{"name":"dDepartment of Molecular Genetics, Faculty of Medicine, Kyoto University, Kyoto 606-8507, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuo","family":"Tanne","sequence":"additional","affiliation":[{"name":"bFrom the Department of Orthodontics, Hiroshima University School of Dentistry, Hiroshima 734-8553, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norihiko","family":"Maeda","sequence":"additional","affiliation":[{"name":"aFrom the Department of Anatomy, Hiroshima University School of Dentistry, Hiroshima 734-8553, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shin-Ichi","family":"Nishikawa","sequence":"additional","affiliation":[{"name":"dDepartment of Molecular Genetics, Faculty of Medicine, Kyoto University, Kyoto 606-8507, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroaki","family":"Kodama","sequence":"additional","affiliation":[{"name":"eResearch Center Kyoto, Bayer Yakuhin, Ltd., Kyoto 619-0216, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1999,7,19]]},"reference":[{"key":"2023072508454023000_R1","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1093\/oxfordjournals.jhered.a108657","article-title":"Osteopetrosis, a new recessive skeletal mutation on chromosome 12 of the mouse","volume":"67","author":"Marks","year":"1976","journal-title":"J. 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