{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T14:50:18Z","timestamp":1791903018241,"version":"4.3.4"},"reference-count":47,"publisher":"Rockefeller University Press","issue":"9","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1999,11,1]]},"abstract":"<jats:p>TECK (thymus-expressed chemokine), a recently described CC chemokine expressed in thymus and small intestine, was found to mediate chemotaxis of human G protein\u2013coupled receptor GPR-9-6\/L1.2 transfectants. This activity was blocked by anti\u2013GPR-9-6 monoclonal antibody (mAb) 3C3. GPR-9-6 is expressed on a subset of memory \u03b14\u03b27high intestinal trafficking CD4 and CD8 lymphocytes. In addition, all intestinal lamina propria and intraepithelial lymphocytes express GPR-9-6. In contrast, GPR-9-6 is not displayed on cutaneous lymphocyte antigen\u2013positive (CLA+) memory CD4 and CD8 lymphocytes, which traffic to skin inflammatory sites, or on other systemic \u03b14\u03b27\u2212CLA\u2212 memory CD4\/CD8 lymphocytes. The majority of thymocytes also express GPR-9-6, but natural killer cells, monocytes, eosinophils, basophils, and neutrophils are GPR-9-6 negative. Transcripts of GPR-9-6 and TECK are present in both small intestine and thymus. Importantly, the expression profile of GPR-9-6 correlates with migration to TECK of blood T lymphocytes and thymocytes. As migration of these cells is blocked by anti\u2013GPR-9-6 mAb 3C3, we conclude that GPR-9-6 is the principal chemokine receptor for TECK. In agreement with the nomenclature rules for chemokine receptors, we propose the designation CCR-9 for GPR-9-6. The selective expression of TECK and GPR-9-6 in thymus and small intestine implies a dual role for GPR-9-6\/CCR-9, both in T cell development and the mucosal immune response.<\/jats:p>","DOI":"10.1084\/jem.190.9.1241","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:48:33Z","timestamp":1027702113000},"page":"1241-1256","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":392,"title":["Human G Protein\u2013Coupled Receptor Gpr-9-6\/Cc Chemokine Receptor 9 Is Selectively Expressed on Intestinal Homing T Lymphocytes, Mucosal Lymphocytes, and Thymocytes and Is Required for Thymus-Expressed Chemokine\u2013Mediated Chemotaxis"],"prefix":"10.1084","volume":"190","author":[{"given":"Brian A.","family":"Zabel","sequence":"first","affiliation":[{"name":"aLeukoSite, Inc., Cambridge, Massachusetts 02142"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William W.","family":"Agace","sequence":"additional","affiliation":[{"name":"bDivision of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James J.","family":"Campbell","sequence":"additional","affiliation":[{"name":"dLaboratory of Immunology and Vascular Biology, Department of Pathology, Stanford University Medical School, Stanford, California 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heidi M.","family":"Heath","sequence":"additional","affiliation":[{"name":"aLeukoSite, Inc., Cambridge, Massachusetts 02142"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Parent","sequence":"additional","affiliation":[{"name":"aLeukoSite, Inc., Cambridge, Massachusetts 02142"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arthur I.","family":"Roberts","sequence":"additional","affiliation":[{"name":"cDepartment of Medicine, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, New Brunswick, New Jersey 08903"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen C.","family":"Ebert","sequence":"additional","affiliation":[{"name":"cDepartment of Medicine, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, New Brunswick, New Jersey 08903"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nasim","family":"Kassam","sequence":"additional","affiliation":[{"name":"aLeukoSite, Inc., Cambridge, Massachusetts 02142"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shixin","family":"Qin","sequence":"additional","affiliation":[{"name":"aLeukoSite, Inc., Cambridge, Massachusetts 02142"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Zovko","sequence":"additional","affiliation":[{"name":"aLeukoSite, Inc., Cambridge, Massachusetts 02142"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory J.","family":"LaRosa","sequence":"additional","affiliation":[{"name":"aLeukoSite, Inc., Cambridge, Massachusetts 02142"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Li","family":"Yang","sequence":"additional","affiliation":[{"name":"aLeukoSite, Inc., Cambridge, Massachusetts 02142"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dulce","family":"Soler","sequence":"additional","affiliation":[{"name":"aLeukoSite, Inc., Cambridge, Massachusetts 02142"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugene C.","family":"Butcher","sequence":"additional","affiliation":[{"name":"dLaboratory of Immunology and Vascular Biology, Department of Pathology, Stanford University Medical School, Stanford, California 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul D.","family":"Ponath","sequence":"additional","affiliation":[{"name":"aLeukoSite, Inc., Cambridge, Massachusetts 02142"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina M.","family":"Parker","sequence":"additional","affiliation":[{"name":"bDivision of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David P.","family":"Andrew","sequence":"additional","affiliation":[{"name":"aLeukoSite, Inc., Cambridge, Massachusetts 02142"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[1999,11,1]]},"reference":[{"key":"2023072509004201900_R1","first-page":"229","article-title":"Chemokines, inflammation and the immune system","volume":"1","author":"Taub","year":"1994","journal-title":"Ther. Immunol"},{"key":"2023072509004201900_R2","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1084\/jem.184.3.799","article-title":"Chemokine receptors and T cell chemotaxis","volume":"184","author":"Mackay","year":"1996","journal-title":"J. Exp. Med"},{"key":"2023072509004201900_R3","doi-asserted-by":"crossref","first-page":"1931","DOI":"10.1172\/JCI118625","article-title":"T lymphocyte recruitment by interleukin-8 (IL-8)","volume":"97","author":"Taub","year":"1996","journal-title":"J. Clin. Invest"},{"key":"2023072509004201900_R4","doi-asserted-by":"crossref","first-page":"5769","DOI":"10.4049\/jimmunol.155.12.5769","article-title":"Controlled recruitment of monocytes and macrophages to specific organs through transgenic expression of monocyte chemoattractant protein-1","volume":"155","author":"Fuentes","year":"1995","journal-title":"J. Immunol"},{"key":"2023072509004201900_R5","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1084\/jem.178.1.367","article-title":"Rapid G protein\u2013regulated activation event involved in lymphocyte binding to high endothelial venules","volume":"178","author":"Bargatze","year":"1993","journal-title":"J. Exp. Med."},{"key":"2023072509004201900_R6","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1038\/365654a0","article-title":"Prevention of lung reperfusion injury in rabbits by a monoclonal antibody against interleukin-8","volume":"365","author":"Sekido","year":"1993","journal-title":"Nature"},{"key":"2023072509004201900_R7","doi-asserted-by":"crossref","first-page":"5003","DOI":"10.4049\/jimmunol.155.10.5003","article-title":"An important role for the chemokine macrophage inflammatory protein-1 alpha in the pathogenesis of the T cell-mediated autoimmune disease, experimental autoimmune encephalomyelitis","volume":"155","author":"Karpus","year":"1995","journal-title":"J. Immunol."},{"key":"2023072509004201900_R8","doi-asserted-by":"crossref","first-page":"7799","DOI":"10.1073\/pnas.92.17.7799","article-title":"A potent inhibitor of endothelial cell proliferation is generated by proteolytic cleavage of the chemokine platelet factor 4","volume":"92","author":"Gupta","year":"1995","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072509004201900_R9","doi-asserted-by":"crossref","first-page":"4333","DOI":"10.4049\/jimmunol.151.8.4333","article-title":"Macrophage inflammatory protein-1 alpha rapidly modulates its receptors and inhibits the anti-CD3 mAb-mediated proliferation of T lymphocytes","volume":"151","author":"Zhou","year":"1993","journal-title":"J. Immunol."},{"key":"2023072509004201900_R10","doi-asserted-by":"crossref","first-page":"2095","DOI":"10.4049\/jimmunol.156.6.2095","article-title":"Chemokines and T lymphocyte activation. I. Beta chemokines costimulate human T lymphocyte activation in vitro","volume":"156","author":"Taub","year":"1996","journal-title":"J. Immunol."},{"key":"2023072509004201900_R11","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1016\/S0092-8674(00)81313-6","article-title":"The \u03b2-chemokine receptors CCR3 and CCR5 facilitate infection by primary HIV-1 isolates","volume":"85","author":"Choe","year":"1996","journal-title":"Cell"},{"key":"2023072509004201900_R12","doi-asserted-by":"crossref","first-page":"872","DOI":"10.1126\/science.272.5263.872","article-title":"HIV-1 entry cofactorfunctional cDNA cloning of a seven-transmembrane, G protein-coupled receptor","volume":"272","author":"Feng","year":"1996","journal-title":"Science"},{"key":"2023072509004201900_R13","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1126\/science.272.5258.60","article-title":"Lymphocyte homing and homeostasis","volume":"272","author":"Butcher","year":"1996","journal-title":"Science."},{"key":"2023072509004201900_R14","first-page":"1461","article-title":"The cutaneous lymphocyte antigen is a skin lymphocyte homing receptor for the vascular lectin endothelial cell leukocyte adhesion molecule-1","volume":"174","author":"Berg","year":"1991","journal-title":"Nature"},{"key":"2023072509004201900_R15","doi-asserted-by":"crossref","first-page":"3282","DOI":"10.4049\/jimmunol.152.7.3282","article-title":"Role of \u03b14 integrins in lymphocyte homing to mucosal tissues in vivo","volume":"152","author":"Hamman","year":"1994","journal-title":"J. Immunol"},{"key":"2023072509004201900_R16","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/1074-7613(95)90162-0","article-title":"Distinct roles of L-selectin and integrins \u03b14\u03b27 and LFA-1 in lymphocyte homing to Peyer's patch-HEV in situthe multistep model confirmed and refined","volume":"3","author":"Bargatze","year":"1995","journal-title":"Immunity"},{"key":"2023072509004201900_R17","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1126\/science.279.5349.381","article-title":"Chemokines and the arrest of lymphocytes rolling under flow conditions","volume":"279","author":"Campbell","year":"1998","journal-title":"Science."},{"key":"2023072509004201900_R18","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1002\/jlb.65.1.6","article-title":"Chemokinessignal lamps for trafficking of T and B cells for development and effector function","volume":"65","author":"Chang","year":"1999","journal-title":"J. Leukoc. Biol"},{"key":"2023072509004201900_R19","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1002\/eji.1830260320","article-title":"Expression of monocyte chemoattractant protein-1 and IL-8 receptors on subsets of T cellscorrelation with transendothelial chemotactic potential","volume":"26","author":"Qin","year":"1996","journal-title":"Eur. J. Immunol"},{"key":"2023072509004201900_R20","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1172\/JCI1422","article-title":"The chemokine receptors CXCR3 and CCR5 mark subsets of T cells associated with certain inflammatory reactions","volume":"101","author":"Qin","year":"1998","journal-title":"J. Clin. 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The role of T cells, chemokines, and adhesion receptors","volume":"98","author":"Gonzalo","year":"1996","journal-title":"J. Clin. Invest."},{"key":"2023072509004201900_R24","doi-asserted-by":"crossref","first-page":"5663","DOI":"10.4049\/jimmunol.161.10.5663","article-title":"Regulation of T lymphocyte trafficking into lymph nodes during an immune response by the chemokines macrophage inflammatory protein (MIP)-1\u03b1 and MIP-1\u03b2","volume":"161","author":"Tedla","year":"1998","journal-title":"J. Immunol."},{"key":"2023072509004201900_R25","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1084\/jem.187.1.129","article-title":"Differential expression of chemokine receptors and chemotactic responsiveness of type 1 T helper cells (Th1s) and Th2s","volume":"187","author":"Bonecchi","year":"1998","journal-title":"J. Exp. Med"},{"key":"2023072509004201900_R26","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1084\/jem.187.6.875","article-title":"Flexible programs of chemokine receptor expression on human polarized T helper 1 and 2 lymphocytes","volume":"187","author":"Sallusto","year":"1998","journal-title":"J. Exp. Med."},{"key":"2023072509004201900_R27","doi-asserted-by":"crossref","first-page":"2005","DOI":"10.1126\/science.277.5334.2005","article-title":"Selective expression of the eotaxin receptor CCR3 by human T helper 2 cells","volume":"277","author":"Sallusto","year":"1997","journal-title":"Science."},{"key":"2023072509004201900_R28","doi-asserted-by":"crossref","first-page":"5027","DOI":"10.4049\/jimmunol.161.9.5027","article-title":"STCP-1 (MDC) CC chemokine acts specifically on chronically activated Th2 lymphocytes and is produced by monocytes on stimulation with Th2 cytokines IL-4 and IL-13","volume":"161","author":"Andrew","year":"1998","journal-title":"J. Immunol."},{"key":"2023072509004201900_R29","doi-asserted-by":"crossref","first-page":"547","DOI":"10.4049\/jimmunol.161.2.547","article-title":"The chemokine receptor CCR8 is preferentially expressed in Th2 but not Th1 cells","volume":"161","author":"Zingoni","year":"1998","journal-title":"J. Immunol."},{"key":"2023072509004201900_R30","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1038\/315672a0","article-title":"Interferon gamma transcriptionally regulates an early response gene containing homology to platelet proteins","volume":"315","author":"Luster","year":"1985","journal-title":"Nature"},{"key":"2023072509004201900_R31","first-page":"148","article-title":"Examination of the low proliferative capacity of human jejunal intra-epithelial lymphocytes","volume":"65","author":"Ebert","year":"1986","journal-title":"Clin. Exp. Immunol."},{"key":"2023072509004201900_R32","doi-asserted-by":"crossref","first-page":"3128","DOI":"10.1021\/bi00226a021","article-title":"Chemical synthesis, purification, and characterization of two inflammatory proteins, neutrophil activating peptide 1 (interleukin-8) and neutrophil activating peptide","volume":"30","author":"Clark-Lewis","year":"1991","journal-title":"Biochemistry"},{"key":"2023072509004201900_R33","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1084\/jem.183.3.901","article-title":"Reversibility of T helper 1 and 2 populations is lost after long term stimulation","volume":"183","author":"Murphy","year":"1997","journal-title":"J. Exp. Med."},{"key":"2023072509004201900_R34","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1083\/jcb.141.4.1053","article-title":"6-C-kine (SLC), a lymphocytes adhesion-triggering chemokine expressed by high endothelium, is an agonist for the MIP-3\u03b2 receptor CCR7","volume":"141","author":"Campbell","year":"1998","journal-title":"J. Cell Biol"},{"key":"2023072509004201900_R35","volume-title":"Molecular CloningA Laboratory Manual","author":"Sambrook","year":"1989","edition":"2nd ed"},{"key":"2023072509004201900_R36","doi-asserted-by":"crossref","first-page":"2437","DOI":"10.1084\/jem.183.6.2437","article-title":"Molecular cloning and characterization of a human eotaxin receptor expressed selectively on eosinophils","volume":"183","author":"Ponath","year":"1996","journal-title":"J. Exp. Med"},{"key":"2023072509004201900_R37","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1038\/304030a0","article-title":"A cell surface molecule involved in organ specific homing of lymphocytes","volume":"304","author":"Gallitin","year":"1983","journal-title":"Nature"},{"key":"2023072509004201900_R38","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1002\/eji.1830260427","article-title":"Distribution of \u03b14\u03b27 and \u03b1EB7 integrins on thymocytes, intestinal epithelial lymphocytes and peripheral lymphocytes","volume":"26","author":"Andrew","year":"1996","journal-title":"Eur. J. Immunol"},{"key":"2023072509004201900_R39","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1016\/S0092-8674(00)81032-6","article-title":"Susceptibility to infection and altered hematopoiesis in mice deficient in both P and E selectins","volume":"84","author":"Frenette","year":"1996","journal-title":"Cell."},{"key":"2023072509004201900_R40","doi-asserted-by":"crossref","first-page":"963","DOI":"10.4049\/jimmunol.161.2.963","article-title":"CD4 T cells migrate into inflamed skin only if they express ligands for E- and P-selectin","volume":"161","author":"Tietz","year":"1998","journal-title":"J. Immunol."},{"key":"2023072509004201900_R41","doi-asserted-by":"crossref","first-page":"3247","DOI":"10.4049\/jimmunol.145.10.3247","article-title":"Differential expression of homing associated adhesion molecules by T cell subsets in man","volume":"145","author":"Picker","year":"1990","journal-title":"J. Immunol."},{"key":"2023072509004201900_R42","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/S1074-7613(00)80531-2","article-title":"TECKa novel CC chemokine specifically expressed by thymic dendritic cells and potentially involved in T cell development","volume":"7","author":"Vicari","year":"1997","journal-title":"Immunity"},{"key":"2023072509004201900_R43","doi-asserted-by":"crossref","first-page":"6273","DOI":"10.4049\/jimmunol.161.11.6273","article-title":"Identification of a truncated form of the CC chemokine CKB-8 demonstrating greatly enhanced biological activity","volume":"161","author":"Macphee","year":"1998","journal-title":"J. Immunol"},{"key":"2023072509004201900_R44","doi-asserted-by":"crossref","first-page":"776","DOI":"10.1038\/23495","article-title":"The chemokine receptor CCR4 in vascular recognition by cutaneous but not intestinal memory T cells","volume":"400","author":"Campbell","year":"1999","journal-title":"Nature."},{"key":"2023072509004201900_R45","doi-asserted-by":"crossref","first-page":"2591","DOI":"10.1002\/eji.1830211041","article-title":"Expression and regulation of \u03b27(\u03b2p) integrins on mouse lymphocytesrelevance to the mucosal immune system","volume":"21","author":"Kilshaw","year":"1991","journal-title":"Eur. J. Immunol"},{"key":"2023072509004201900_R46","first-page":"3192","article-title":"Activation of blood T lymphocytes down-regulates CXCR4 expression and interferes with propagation of X4 HIV strains","volume":"28","author":"Bermejo","year":"1998","journal-title":"J. Immunol."},{"key":"2023072509004201900_R47","doi-asserted-by":"crossref","first-page":"5671","DOI":"10.4049\/jimmunol.162.10.5671","article-title":"Identification of the orphan chemokine receptor GPR-9-6 as CCR9, the receptor for the chemokine TECK","volume":"162","author":"Zaballos","year":"1999","journal-title":"J. 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