{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T17:32:31Z","timestamp":1761154351234},"reference-count":51,"publisher":"Rockefeller University Press","issue":"1","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2000,1,3]]},"abstract":"<jats:p>The MC148 CC chemokine from the human poxvirus molluscum contagiosum (MCV) was probed in parallel with viral macrophage inflammatory protein (vMIP)-II encoded by human herpesvirus 8 (HHV8) in 16 classified human chemokine receptors. In competition binding using radiolabeled endogenous chemokines as well as radiolabeled MC148, MC148 bound with high affinity only to CCR8. In calcium mobilization assays, MC148 had no effect on its own on any of the chemokine receptors, but in a dose-dependent manner blocked the stimulatory effect of the endogenous I-309 chemokine on CCR8 without affecting chemokine-induced signaling of any other receptor. In contrast, vMIP-II acted as an antagonist on 10 of the 16 chemokine receptors, covering all four classes: XCR, CCR, CXCR, and CX3CR. In chemotaxis assays, MC148 specifically blocked the I-309\u2013induced response but, for example, not stromal cell\u2013derived factor 1\u03b1, monocyte chemoattractant protein 1, or interleukin 8\u2013induced chemotaxis. We thus concluded that the two viruses choose two different ways to block the chemokine system: HHV8 encodes the broad-spectrum chemokine antagonist vMIP-II, whereas MCV encodes a highly selective CCR8 antagonist, MC148, conceivably to interfere with monocyte invasion and dendritic cell function. Because of its pharmacological selectivity, the MC148 protein could be a useful tool in the delineation of the role played by CCR8 and its endogenous ligand, I-309.<\/jats:p>","DOI":"10.1084\/jem.191.1.171","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:48:33Z","timestamp":1027702113000},"page":"171-180","update-policy":"http:\/\/dx.doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":125,"title":["A Highly Selective Cc Chemokine Receptor (Ccr)8 Antagonist Encoded by the Poxvirus Molluscum Contagiosum"],"prefix":"10.1084","volume":"191","author":[{"given":"Hans R.","family":"L\u00fcttichau","sequence":"first","affiliation":[{"name":"aFrom the Laboratory for Molecular Pharmacology, Panum Institute, DK-2200 Copenhagen, Denmark"},{"name":"bDepartment of Infectious Diseases, Rigshospitalet, DK-2100 Copenhagen, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johnny","family":"Stine","sequence":"additional","affiliation":[{"name":"dICOS Corporation, Seattle, Washington 98021"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas P.","family":"Boesen","sequence":"additional","affiliation":[{"name":"aFrom the Laboratory for Molecular Pharmacology, Panum Institute, DK-2200 Copenhagen, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anders H.","family":"Johnsen","sequence":"additional","affiliation":[{"name":"cDepartment of Clinical Biochemistry, Rigshospitalet, DK-2100 Copenhagen, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Chantry","sequence":"additional","affiliation":[{"name":"dICOS Corporation, Seattle, Washington 98021"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Gerstoft","sequence":"additional","affiliation":[{"name":"bDepartment of Infectious Diseases, Rigshospitalet, DK-2100 Copenhagen, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thue W.","family":"Schwartz","sequence":"additional","affiliation":[{"name":"aFrom the Laboratory for Molecular Pharmacology, Panum Institute, DK-2200 Copenhagen, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[2000,1,3]]},"reference":[{"key":"2023072509075390100_R1","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1111\/j.1600-0560.1990.tb00085.x","article-title":"Immunocompetent cells and epithelial cell modifications in molluscum contagiosum","volume":"17","author":"Viac","year":"1990","journal-title":"J. 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