{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T20:20:41Z","timestamp":1771532441549,"version":"3.50.1"},"reference-count":37,"publisher":"Rockefeller University Press","issue":"11","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2000,6,5]]},"abstract":"<jats:p>Whereas CD40\u2013CD40 ligand interactions are important for various dendritic cell (DC) functions in vitro, their in vivo relevance is unknown. We analyzed the DC status of CD40 ligand \u2212\/\u2212 mice using a contact hypersensitivity (CHS) model system that enables multiple functions of DCs to be assessed in vivo. Immunohistochemistry of skin sections revealed no differences in terms of numbers and morphology of dendritic epidermal Langerhans cells (LCs) in unsensitized CD40 ligand \u2212\/\u2212 mice as compared with wild-type C57BL\/6 mice. However, after contact sensitization of CD40 ligand \u2212\/\u2212 mice, LCs failed to migrate out of the skin and substantially fewer DCs accumulated in draining lymph nodes (DLNs). Furthermore, very few antigen-bearing DCs could be detected in the paracortical region of lymph nodes draining sensitized skin. This defect in DC migration after hapten sensitization was associated with defective CHS responses and decreased cutaneous tumor necrosis factor (TNF)-\u03b1 production and was corrected by injecting recombinant TNF-\u03b1 or an agonistic anti-CD40 monoclonal antibody. Thus, CD40\u2013CD40 ligand interactions in vivo regulate the migration of antigen-bearing DCs from the skin to DLNs via TNF-\u03b1 production and play a vital role in the initiation of acquired T cell\u2013mediated immunity.<\/jats:p>","DOI":"10.1084\/jem.191.11.2011","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T16:48:33Z","timestamp":1027702113000},"page":"2011-2020","update-policy":"https:\/\/doi.org\/10.1084\/jem.crossmarkpolicy","source":"Crossref","is-referenced-by-count":99,"title":["Cd40\u2013Cd40 Ligand Interactions in Vivo Regulate Migration of Antigen-Bearing Dendritic Cells from the Skin to Draining Lymph Nodes"],"prefix":"10.1084","volume":"191","author":[{"given":"Angus M.","family":"Moodycliffe","sequence":"first","affiliation":[{"name":"aDepartment of Immunology-178, M.D. Anderson Cancer Center, Houston, Texas 77030"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vijay","family":"Shreedhar","sequence":"additional","affiliation":[{"name":"cGI Research Laboratory, Harvard Medical School, Boston, Massachusetts 02115"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen E.","family":"Ullrich","sequence":"additional","affiliation":[{"name":"aDepartment of Immunology-178, M.D. Anderson Cancer Center, Houston, Texas 77030"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey","family":"Walterscheid","sequence":"additional","affiliation":[{"name":"aDepartment of Immunology-178, M.D. Anderson Cancer Center, Houston, Texas 77030"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Corazon","family":"Bucana","sequence":"additional","affiliation":[{"name":"bDepartment of Cancer Biology-173, M.D. Anderson Cancer Center, Houston, Texas 77030"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margaret L.","family":"Kripke","sequence":"additional","affiliation":[{"name":"aDepartment of Immunology-178, M.D. Anderson Cancer Center, Houston, Texas 77030"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leopoldo","family":"Flores-Romo","sequence":"additional","affiliation":[{"name":"aDepartment of Immunology-178, M.D. Anderson Cancer Center, Houston, Texas 77030"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[1999,6,6]]},"reference":[{"key":"2023072509014660000_R1","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1038\/32588","article-title":"Dendritic cells and the control of immunity","volume":"392","author":"Banchereau","year":"1998","journal-title":"Nature"},{"key":"2023072509014660000_R2","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1146\/annurev.iy.09.040191.001415","article-title":"The dendritic cell system and its role in immunogenicity","volume":"9","author":"Steinman","year":"1991","journal-title":"Annu. 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